Metabolomics and correlation network analysis of follicular fluid reveals associations between l ‐tryptophan, l ‐tyrosine and polycystic ovary syndrome

2020 ◽  
Author(s):  
Entai Hou ◽  
Yue Zhao ◽  
Jing Hang ◽  
Jie Qiao
Keyword(s):  
Network Analysis ◽  
Polycystic Ovary Syndrome ◽  
Follicular Fluid ◽  
Polycystic Ovary ◽  
Correlation Network ◽  
Ovary Syndrome

scholarly journals Correlation between steroid levels in follicular fluid and hormone synthesis related substances in its exosomes and embryo quality in patients with polycystic ovary syndrome

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Li Yu ◽  
Miao Liu ◽  
Zhenxin Wang ◽  
Te Liu ◽  
Suying Liu ◽  
...  
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Follicular Fluid ◽  
Polycystic Ovary ◽  
Mrna Levels ◽  
Hormonal Changes ◽  
Male Factor ◽  
Expression Change ◽  
Ovary Syndrome ◽  
Steroid Synthesis ◽  
Pcos Group

Abstract Background Polycystic ovary syndrome (PCOS) is an endocrine and metabolic disorder with various manifestations and complex etiology. Follicular fluid (FF) serves as the complex microenvironment for follicular development. However, the correlation between the concentration of steroid in FF and the pathogenesis of PCOS is still unclear. Methods Twenty steroid levels in FF from ten patients with PCOS and ten women with male-factor infertility undergoing in vitro fertilization were tested by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in order to explore their possibly correlation with PCOS. Meanwhile, the mRNA levels of core enzymes in steroid synthesis pathway from exosomes of FF were also detected by qPCR. Results The estriol (p < 0.01), estradiol (p < 0.05) and prenenolone (p < 0.01) levels in FF of PCOS group were significantly increased, compared to the normal group, and the progesterone levels (p < 0.05) were decreased in PCOS group. Increased mRNA levels of CYP11A, CYP19A and HSD17B2 of exosomes were accompanied by the hormonal changes in FF. Correlation analysis showed that mRNA levels of CYP11A and HSD17B2 were negatively correlated with percent of top-quality embryos and rate of embryos develop to blastocyst. Conclusion Our results suggest that increased levels of estrogen and pregnenolone in follicular fluid may affect follicle development in PCOS patients, and the mechanism is partially related to HSD17B1, CYP19A1 and CYP11A1 expression change in FF exosomes.

Follicular fluid total antioxidant capacity and ischemia modified albumin levels in polycystic ovary syndrome

2014 ◽  
Vol 102 (3) ◽  
pp. e278
Author(s):  
I.N.B. Duzguner ◽  
Y. Tasci ◽  
G.S. Caglar ◽  
B. Dilbaz ◽  
S. Demirtas ◽  
...  
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Antioxidant Capacity ◽  
Follicular Fluid ◽  
Total Antioxidant Capacity ◽  
Polycystic Ovary ◽  
Ovary Syndrome ◽  
Ischemia Modified Albumin ◽  
Total Antioxidant

Polycystic ovary syndrome: brain-derived neurotrophic factor (BDNF) plasma and follicular fluid levels

2012 ◽  
Vol 28 (4) ◽  
pp. 241-244 ◽  
Author(s):  
N. Russo ◽  
M. Russo ◽  
D. Daino ◽  
F. Bucci ◽  
N. Pluchino ◽  
...  
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Follicular Fluid ◽  
Neurotrophic Factor ◽  
Polycystic Ovary ◽  
Brain Derived Neurotrophic Factor ◽  
Ovary Syndrome ◽  
Fluid Levels

Follicular fluid norepinephrine and dopamine concentrations are higher in polycystic ovary syndrome

2016 ◽  
Vol 32 (6) ◽  
pp. 460-463 ◽  
Author(s):  
Natı Musalı ◽  
Batuhan Özmen ◽  
Yavuz Emre Şükür ◽  
Berrin İmge Ergüder ◽  
Cem Somer Atabekoğlu ◽  
...  
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Follicular Fluid ◽  
Polycystic Ovary ◽  
Ovary Syndrome

scholarly journals LncRNA TMPO-AS1 suppresses the maturation of miR-335-5p to participate in polycystic ovary syndrome

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Fang Hou ◽  
Jie Li ◽  
Jie Peng ◽  
Zhenghua Teng ◽  
Jun Feng ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cell Proliferation ◽  
Polycystic Ovary Syndrome ◽  
Follicular Fluid ◽  
Polycystic Ovary ◽  
Subcellular Location ◽  
Ovary Syndrome ◽  
Brdu Assay ◽  
Transfection Assay

Abstract Background TMPO-AS1 is a recently characterized oncogenic lncRNA in ovarian cancer. Its role in other ovary diseases is unknown. This study explored its role in polycystic ovary syndrome (PCOS). Methods Follicular fluid was extracted from both PCOS patients and controls. The levels of TMPO-AS1 and mature and premature miR-335-5p were analyzed by RT-qPCR. The role of TMPO-AS1 in regulating miR-355-5p maturation in granulosa-like tumor (KGN) cells was analyzed by overexpression experiments. The interaction between TMPO-AS1 and premature miR-335-5p was analyzed by RNA pull-down assay. The subcellular location of TMPO-AS1 in KGN cells was analyzed by nuclear fractionation assay. The role of TMPO-AS1 and miR-335-5p in KGN cell proliferation was analyzed by BrdU assay. Results TMPO-AS1 was increased in PCOS, while mature miR-355-5p was decreased in PCOS. TMPO-AS1 overexpression decreased mature miR-355-5p level but increased premature miR-355-5p. TMPO-AS1 was localized in both nucleus and cytoplasm. TMPO-AS1 directly interacted with premature miR-355-5p in KGN cells. TMPO-AS1 increased KGN cell proliferation while miR-355-5p decreased cell proliferation. The co-transfection assay showed that TMPO-AS1 reduced the suppressive effects of miR-355-5p on cell proliferation. Conclusions TMPO-AS1 might suppress miR-335-5p maturation to participate in PCOS.

scholarly journals S100‐A9 protein in exosomes derived from follicular fluid promotes inflammation via activation of NF‐κB pathway in polycystic ovary syndrome

2020 ◽  
Vol 24 (1) ◽  
pp. 114-125 ◽  
Author(s):  
Han Li ◽  
Xin Huang ◽  
Xinwen Chang ◽  
Julei Yao ◽  
Qizhi He ◽  
...  
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Follicular Fluid ◽  
Polycystic Ovary ◽  
Ovary Syndrome

High copper levels in follicular fluid affect follicle development in polycystic ovary syndrome patients: Population-based and in vitro studies

2019 ◽  
Vol 365 ◽  
pp. 101-111 ◽  
Author(s):  
Yan Sun ◽  
Wenxiang Wang ◽  
Yiwei Guo ◽  
Beihong Zheng ◽  
Hong Li ◽  
...  
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Follicular Fluid ◽  
Polycystic Ovary ◽  
Population Based ◽  
In Vitro Studies ◽  
Follicle Development ◽  
Ovary Syndrome ◽  
High Copper

scholarly journals Protein modification as oxidative stress marker in follicular fluid from women with polycystic ovary syndrome: the effect of inositol and metformin

2014 ◽  
Vol 31 (10) ◽  
pp. 1269-1276 ◽  
Author(s):  
P. Piomboni ◽  
R. Focarelli ◽  
A. Capaldo ◽  
A. Stendardi ◽  
V. Cappelli ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Polycystic Ovary Syndrome ◽  
Follicular Fluid ◽  
Protein Modification ◽  
Polycystic Ovary ◽  
Oxidative Stress Marker ◽  
Ovary Syndrome ◽  
Stress Marker

scholarly journals Leukaemia inhibitory factor in serum and follicular fluid of women with polycystic ovary syndrome and its correlation with IVF outcome

2018 ◽  
Vol 36 (4) ◽  
pp. 483-489 ◽  
Author(s):  
Zewu Li ◽  
Yueting Zhu ◽  
Hongchang Li ◽  
Wenjie Jiang ◽  
Hongbin Liu ◽  
...  
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Follicular Fluid ◽  
Polycystic Ovary ◽  
Inhibitory Factor ◽  
Leukaemia Inhibitory Factor ◽  
Ivf Outcome ◽  
Ovary Syndrome
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