scholarly journals A protocol to transfer a fed‐batch platform process into semi‐perfusion mode: The benefit of automated small‐scale bioreactors compared to shake flasks as scale‐down model

2018 ◽  
Vol 35 (2) ◽  
pp. e2757 ◽  
Author(s):  
Sabrina Janoschek ◽  
Markus Schulze ◽  
Gerben Zijlstra ◽  
Gerhard Greller ◽  
Jens Matuszczyk
Keyword(s):  
Small Scale ◽  
Fed Batch ◽  
Platform Process ◽  
Scale Down ◽  
Shake Flasks ◽  
Perfusion Mode

The impact of pH inhomogeneities on CHO cell physiology and fed-batch process performance - two-compartment scale-down modelling and intracellular pH excursion

2017 ◽  
Vol 12 (7) ◽  
pp. 1600633 ◽  
Author(s):  
Matthias Brunner ◽  
Philipp Braun ◽  
Philipp Doppler ◽  
Christoph Posch ◽  
Dirk Behrens ◽  
...  
Keyword(s):  
Intracellular Ph ◽  
Batch Process ◽  
Cell Physiology ◽  
Process Performance ◽  
Fed Batch ◽  
Cho Cell ◽  
Scale Down ◽  
The Impact

Equalizing growth in high-throughput small scale cultivations via precultures operated in fed-batch mode

2009 ◽  
Vol 103 (6) ◽  
pp. 1095-1102 ◽  
Author(s):  
Robert Huber ◽  
Marco Scheidle ◽  
Barbara Dittrich ◽  
Doris Klee ◽  
Jochen Büchs
Keyword(s):  
High Throughput ◽  
Small Scale ◽  
Fed Batch ◽  
Batch Mode

scholarly journals Description of a Decentralized Small Scale Digester for Treating Organic Wastes

Environments ◽  
2020 ◽  
Vol 7 (10) ◽  
pp. 78
Author(s):  
Rubén González ◽  
Daniel Blanco ◽  
Judith González-Arias ◽  
José García-Cascallana ◽  
Xiomar Gómez
Keyword(s):  
Organic Loading Rate ◽  
Small Scale ◽  
Complex Nature ◽  
Fed Batch ◽  
Batch Mode ◽  
Commercial Unit ◽  
Start Up ◽  
Volatile Solids ◽  
Vegetable Wastes ◽  
Feeding Substrate

This manuscript deals with the detailed design of a small digestion prototype intended as a commercial unit fully operational to cover the demand for decentralized treatment of wastes. These plants are highly affected by the complex nature of wastes giving rise to different operating problems that should be considered in detail. This paper describes the design and start-up strategy of a small-scale digestion plant with a volume of 8 m3 designed to operate with a hydrolysis pretreatment unit. The plant was designed to treat fruit and vegetable wastes as substrates derived from a local processing food factory. The performance of the plant during fed-batch operation was reported. The strategy of inoculating the reactor only to a third of its original volume and subsequently increasing the volume of the reactor by using the fed-batch mode was inadequate. The acid pH of the feeding substrate resulted in the application of a low organic loading rate with a volumetric variation of just 19.7 L/d. The performance of the plant was evaluated at non-steady state conditions and resulted in excessive destruction of volatile solids due to the low nitrogen content of the feeding substrate. The prototype reported a specific methane production of 232 L/kg volatile solids despite the low feeding rate supplemented.

scholarly journals Automated Conditional Screening of Multiple Escherichia coli Strains in Parallel Adaptive Fed-Batch Cultivations

2020 ◽  
Vol 7 (4) ◽  
pp. 145
Author(s):  
Sebastian Hans ◽  
Benjamin Haby ◽  
Niels Krausch ◽  
Tilman Barz ◽  
Peter Neubauer ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Developmental Stages ◽  
Added Value ◽  
Major Influence ◽  
Cell Factory ◽  
Sufficient Information ◽  
Limited Information ◽  
Fed Batch ◽  
Scale Down ◽  
Oscillating Glucose

In bioprocess development, the host and the genetic construct for a new biomanufacturing process are selected in the early developmental stages. This decision, made at the screening scale with very limited information about the performance in larger reactors, has a major influence on the efficiency of the final process. To overcome this, scale-down approaches during screenings that show the real cell factory performance at industrial-like conditions are essential. We present a fully automated robotic facility with 24 parallel mini-bioreactors that is operated by a model-based adaptive input design framework for the characterization of clone libraries under scale-down conditions. The cultivation operation strategies are computed and continuously refined based on a macro-kinetic growth model that is continuously re-fitted to the available experimental data. The added value of the approach is demonstrated with 24 parallel fed-batch cultivations in a mini-bioreactor system with eight different Escherichia coli strains in triplicate. The 24 fed-batch cultivations were run under the desired conditions, generating sufficient information to define the fastest-growing strain in an environment with oscillating glucose concentrations similar to industrial-scale bioreactors.

Fed-Batch Cultivation in Baffled Shake Flasks

2011 ◽  
Vol 31 (14) ◽  
pp. 52-54 ◽  
Author(s):  
Johannes Hemmerich,
Keyword(s):  
Batch Cultivation ◽  
Fed Batch ◽  
Shake Flasks ◽  
Fed Batch Cultivation

Enhanced production of the nonribosomal peptide antibiotic valinomycin in Escherichia coli through small-scale high cell density fed-batch cultivation

2013 ◽  
Vol 98 (2) ◽  
pp. 591-601 ◽  
Author(s):  
Jian Li ◽  
Jennifer Jaitzig ◽  
Friederike Hillig ◽  
Roderich Süssmuth ◽  
Peter Neubauer
Keyword(s):  
Escherichia Coli ◽  
Cell Density ◽  
High Cell Density ◽  
Batch Cultivation ◽  
Small Scale ◽  
Peptide Antibiotic ◽  
High Cell ◽  
Fed Batch ◽  
Enhanced Production ◽  
Fed Batch Cultivation

scholarly journals Modelling concentration gradients in fed-batch cultivations of E. coli  - towards the flexible design of scale-down experiments

2018 ◽  
Vol 94 (2) ◽  
pp. 516-526 ◽  
Author(s):  
Emmanuel Anane ◽  
Annina Sawatzki ◽  
Peter Neubauer ◽  
Mariano Nicolas Cruz-Bournazou
Keyword(s):  
Flexible Design ◽  
Fed Batch ◽  
Concentration Gradients ◽  
E Coli ◽  
Scale Down

Parallel Bubble Columns with Fed-Batch Technique for Microbial Process Development on a Small Scale

1999 ◽  
Vol 22 (12) ◽  
pp. 1051-1058 ◽  
Author(s):  
J. Altenbach-Rehm ◽  
Ch. Nell ◽  
M. Arnold ◽  
D. Weuster-Botz
Keyword(s):  
Process Development ◽  
Bubble Columns ◽  
Small Scale ◽  
Fed Batch ◽  
Microbial Process ◽  
Batch Technique

scholarly journals OPTIMIZATION OF RIFAMYCIN B USING NOCARDIA MEDITERRANEA (ATCC 13865) BY FED BATCH MODE IN SHAKE FLASKS

2017 ◽  
Vol 7 (4) ◽  
Author(s):  
Suneel Kumar Niranjan ◽  
Chandrul Kaushal ◽  
S K Jain
Keyword(s):  
Fed Batch ◽  
Batch Mode ◽  
Shake Flasks ◽  
Rifamycin B
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