Analysis of Tradeoffs between Purification Factor and Yield for High Performance Countercurrent Membrane Purification for Protein Separations

2021 ◽  
Author(s):  
Christopher J. Yehl ◽  
Andrew L. Zydney
Keyword(s):  
High Performance ◽  
Protein Separations ◽  
Purification Factor

Comparison of high-performance ion-exchaneg chromatography and gel electrophoresis in protein separations

1985 ◽  
Vol 318 ◽  
pp. 358-361 ◽  
Author(s):  
Yoshio Kato ◽  
Koji Nakamura ◽  
Yosuke Yamazaki ◽  
Tsutomu Hashimoto
Keyword(s):  
Gel Electrophoresis ◽  
High Performance ◽  
Protein Separations

High-performance protein separations with novel strong ion exchangers

1994 ◽  
Vol 679 (1) ◽  
pp. 11-22 ◽  
Author(s):  
Judit Horvath ◽  
Egisto Boschetti ◽  
Luc Guerrier ◽  
Nelson Cooke
Keyword(s):  
High Performance ◽  
Protein Separations ◽  
Ion Exchangers

Making Sharper Peaks for Reverse-Phase Liquid Chromatography of Proteins

2020 ◽  
Vol 13 (1) ◽  
pp. 363-380 ◽  
Author(s):  
Charles R. Bupp ◽  
Mary J. Wirth
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Genome Project ◽  
Protein Separations ◽  
Basic Principles ◽  
Peak Broadening ◽  
Phase Liquid ◽  
The Many ◽  
The Human Genome Project ◽  
Dramatic Growth

Protein separations have gained increasing interest over the past two decades owing to the dramatic growth of proteins as therapeutics and the completion of the Human Genome Project. About every decade, the field of protein high-performance liquid chromatography (HPLC) seems to mature, having reached what appears to be a theoretical limit. But then scientists well versed in the basic principles of HPLC invented a way around the limit, generating another decade of exciting progress. There is still the need for higher resolution and better compatibility with mass spectrometry because it is an essential tool for identification of proteins and their modifications. To make advances, the fundamental principles need to be understood. This review covers recent advances and current needs in the context of the principles that underlie the many contributions to peak broadening.

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