Making Sharper Peaks for Reverse-Phase Liquid Chromatography of Proteins

2020 ◽  
Vol 13 (1) ◽  
pp. 363-380 ◽  
Author(s):  
Charles R. Bupp ◽  
Mary J. Wirth
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Genome Project ◽  
Protein Separations ◽  
Basic Principles ◽  
Peak Broadening ◽  
Phase Liquid ◽  
The Many ◽  
The Human Genome Project ◽  
Dramatic Growth

Protein separations have gained increasing interest over the past two decades owing to the dramatic growth of proteins as therapeutics and the completion of the Human Genome Project. About every decade, the field of protein high-performance liquid chromatography (HPLC) seems to mature, having reached what appears to be a theoretical limit. But then scientists well versed in the basic principles of HPLC invented a way around the limit, generating another decade of exciting progress. There is still the need for higher resolution and better compatibility with mass spectrometry because it is an essential tool for identification of proteins and their modifications. To make advances, the fundamental principles need to be understood. This review covers recent advances and current needs in the context of the principles that underlie the many contributions to peak broadening.

Glossary of terms used in medicinal chemistry. Part II (IUPAC Recommendations 2013)

2013 ◽  
Vol 85 (8) ◽  
pp. 1725-1758 ◽  
Author(s):  
Derek R. Buckle ◽  
Paul W. Erhardt ◽  
C. Robin Ganellin ◽  
Toshi Kobayashi ◽  
Thomas J. Perun ◽  
...  
Keyword(s):  
Molecular Biology ◽  
Human Genome ◽  
Human Genome Project ◽  
Medicinal Chemistry ◽  
Genome Project ◽  
Scientific Disciplines ◽  
New Concepts ◽  
Essential Components ◽  
The Many ◽  
The Human Genome Project

The evolution that has taken place in medicinal chemistry practice as a result of major advances in genomics and molecular biology arising from the Human Genome Project has carried with it an extensive additional working vocabulary that has become both integrated and essential terminology for the medicinal chemist. Some of this augmented terminology has been adopted from the many related and interlocked scientific disciplines with which the modern medicinal chemist must be conversant, but many other terms have been introduced to define new concepts and ideas as they have arisen. In this supplementary Glossary, we have attempted to collate and define many of the additional terms that are now considered to be essential components of the medicinal chemist’s expanded repertoire.

The Human Genome Project and public policy

1999 ◽  
Vol 8 (3) ◽  
pp. 161-168 ◽  
Author(s):  
Glen A. Evans
Keyword(s):  
Human Genome ◽  
Human Genome Project ◽  
Social Issues ◽  
Genome Project ◽  
Biological Information ◽  
Biological Research ◽  
Genetic Privacy ◽  
Emerging Issues ◽  
The Many ◽  
The Human Genome Project

The Human Genome Project is a massive government and privately funded undertaking to sequence the entire human genome and discover all 80,000 human genes in less than 15 years. As the project nears completion in the first decade of the 21st century, the ramifications of public availability of this vast amount of biological information are likely to pervade society. The legal, ethical and social issues raised by the genome project and associated biological research are expected to have a profound and long lasting impact on daily life. How society deals with the many emerging issues involving genetic privacy, designer babies, and the transformation of medical care among others will be a major focus of public and governmental discussion in the next decade.

scholarly journals Correlation between retention and C-18 silica gel surface coverage in reversed-phase liquid chromatography

2000 ◽  
Vol 65 (11) ◽  
pp. 811-818
Author(s):  
Marijana Acanski ◽  
Slobodan Petrovic ◽  
Vjera Pejanovic ◽  
Julijana Petrovic
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Carbon Content ◽  
Silica Gel ◽  
High Performance ◽  
Surface Coverage ◽  
Reversed Phase ◽  
Reversed Phase Liquid Chromatography ◽  
Retention Behaviour ◽  
Phase Liquid

The effect of C-18 silica gel surface coverage on the retention behaviour of some estrone derivatives in reversed phase high performance liquid chromatography has been studied. Two commercial columns with different C-18 coverage, Spherisorb ODS-1(8 % carbon content) and Li Chrosorb RP-18(22% carbon content), using methanol-water as the eluent, were used.

Mining Microarray Data

2011 ◽  
pp. 810-814
Author(s):  
Nanxiang Ge ◽  
Li Liu
Keyword(s):  
Human Genome ◽  
Sequence Data ◽  
Scientific Discovery ◽  
New Technology ◽  
Genome Project ◽  
Technology Platforms ◽  
Major Application ◽  
Data Explosion ◽  
The Many ◽  
The Human Genome Project

During the last 10 years and in particularly within the last few years, there has been a data explosion associated with the completion of the human genome project (HGP) (IHGMC and Venter et al., 2001) in 2001 and the many sophisticated genomics technologies. The human genome (and genome from other species) now provides an enormous amount of data waiting to be transformed into useful information and scientific knowledge. The availability of genome sequence data also sparks the development of many new technology platforms. Among the available different technology platforms, microarray is one of the technologies that is becoming more and more mature and has been widely used as a tool for scientific discovery. The major application of microarray is for simultaneously measuring the expression level of thousands of genes in the cell. It has been widely used in drug discovery and starts to impact the drug development process.

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