Objective:
Pioglitazone (PG) is used to control high blood sugar in patients with type 2 diabetes
mellitus. PG acts as a peroxisome proliferator-activated receptor γ agonist. In addition to the
insulin-sensitizing effect, PG possesses anti-inflammatory effect. In this study, the protective
effect of PG was evaluated against DNA damage induced by ionizing radiation in human healthy
lymphocytes.
Methods:
The microtubes containing human whole blood were treated with PG at various
concentrations (1-50 μM) for three hours. Then, the blood samples were irradiated with X-ray.
Lymphocytes were cultured for determining the frequency of micronuclei as a genotoxicity
biomarker in binucleated lymphocytes.
Results:
The mean percentage of micronuclei was significantly increased in human lymphocytes
when were exposed to IR, while it was decreased in lymphocytes pre-treated with PG. The
maximum reduction in the frequency of micronuclei in irradiated lymphocytes was observed at 5
μM of PG treatment (48% decrease).
Conclusion:
The anti-inflammatory property is suggested the mechanism action of PG for
protection human lymphocytes against genotoxicity induced by ionizing radiation.
Anti-tumor effects of a nonsteroidal anti-inflammatory drug zaltoprofen on chondrosarcoma via activating peroxisome proliferator-activated receptor gamma and suppressing matrix metalloproteinase-2 expression
