Changes in adenosine deaminase activity in ageing cultured human cells and the role of zinc

2003 ◽  
Vol 21 (3) ◽  
pp. 275-282 ◽  
Author(s):  
H. K. Ghneim ◽  
S. S. Al-Saleh ◽  
F. J. Al-Shammary ◽  
Z. S. Kordee
Keyword(s):  
Adenosine Deaminase ◽  
Human Cells ◽  
Adenosine Deaminase Activity ◽  
Cultured Human Cells

Cytotoxic, genotoxic and cell-cycle disruptive effects of thio-dimethylarsinate in cultured human cells and the role of glutathione

2008 ◽  
Vol 228 (1) ◽  
pp. 59-67 ◽  
Author(s):  
Takafumi Ochi ◽  
Kayoko Kita ◽  
Toshihide Suzuki ◽  
Alice Rumpler ◽  
Walter Goessler ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Human Cells ◽  
Cultured Human Cells

Study of serum adenosine deaminase activity and c-reactive protein in patients of rheumatoid arthritis

2020 ◽  
Vol 11 (4) ◽  
pp. 7990-7993
Author(s):  
Sangeetha R ◽  
Ramesh Raju K A P ◽  
Hemapriya S ◽  
Suganthi V ◽  
Panneerselvam P
Keyword(s):  
Rheumatoid Arthritis ◽  
Adenosine Deaminase ◽  
Treatment Plan ◽  
Colorimetric Method ◽  
Disease Score ◽  
C Reactive Protein ◽  
Positive Correlation ◽  
Reactive Protein ◽  
Adenosine Deaminase Activity

Rheumatoid arthritis (RA) is a chronic disease that causes inflammatory synovitis. The treatment plan of RA includes reducing inflammation and improving the quality of life. Hence, understanding the role of Adenosine deaminase (ADA) and C-reactive protein helps for a better plan of treatment. The present study was undertaken to determine the serum ADA activity and CRP in RA patients and correlate with the severity of the progression of the disease. 25 patients diagnosed with RA as per 2010 ACR/EULAR criteria and 25 age and sex matched healthy controls were included in the study after informed consent. Blood samples were collected from all the subjects after an overnight fast, serum separated was analyzed immediately for Adenosine deaminase(ADA) activity measured using colorimetric method of Guisti and Galanti. Disease score, C-reactive protein, RA factor, ADA and ESR were significantly higher in cases when compared with controls. Significant positive correlation was present between the disease score and C-reactive protein, RA factor among cases. A positive correlation was observed between the disease score and ADA, but it was not statistically significant among cases.

scholarly journals The value of adenosine deaminase (ADA) determination in the diagnosis of tuberculous ascites

1995 ◽  
Vol 37 (5) ◽  
pp. 449-453 ◽  
Author(s):  
Cesar Q. Brant ◽  
Mario R. Silva Jr. ◽  
Erica P. Macedo ◽  
Claudio Vasconcelos ◽  
Natalina Tamaki ◽  
...  
Keyword(s):  
Adenosine Deaminase ◽  
Ascitic Fluid ◽  
Spontaneous Bacterial Peritonitis ◽  
Malignant Ascites ◽  
Bacterial Peritonitis ◽  
Pancreatic Ascites ◽  
Adenosine Deaminase Activity ◽  
Sensitivity Specificity

In order to evaluate the role of the determination of adenosine deaminase activity (ADA) in ascitic fluid for the diagnosis of tuberculosis, 44 patients were studied. Based on biochemical, cytological, histopathological and microbiological tests, the patients were divided into 5 groups: G1 - tuberculous ascites (n = 8); G2 - malignant ascites (n = 13); G3 - spontaneous bacterial peritonitis (n = 6); G4 - pancreatic ascites (n = 2); G5 - miscelaneous ascites (n = 15). ADA concentration were significantly higher in G1 (133.50 ± 24.74 U/l) compared to the other groups (G2 = 41.85 ± 52.07 U/l; G3 = 10.63 ± 5.87 U/l; G4 = 18.00 ± 7.07 U/l; G5 = 11.23 ± 7.66 U/l). At a cut-off value of >31 U/l, the sensitivity, specificity and positive and negative preditive values were 100%, 92%, 72% and 100%, respectively. ADA concentrations as high as in tuberculous ascites were only found in two malignant ascites caused by lymphoma. We conclude that ADA determination in ascitic fluid is a useful and reliable screening test for diagnosing tuberculous ascites. Values of ADA higher than 31 U/l indicate more invasive methods to confirm the diagnosis of tuberculosis.

scholarly journals Adenosine Deaminase Activity in the Serum of Type 2 Diabetic Patients

2004 ◽  
Vol 47 (1) ◽  
pp. 33-35 ◽  
Author(s):  
Naciye Kurtul ◽  
Sadrettin Pençe ◽  
Ersin Akarsu ◽  
Hasan Koçoğlu ◽  
Yemliha Aksoy ◽  
...  
Keyword(s):  
Serum Level ◽  
Adenosine Deaminase ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Insulin Effect ◽  
Type 2 Dm ◽  
Adenosine Deaminase Activity ◽  
Type 2 Diabetic

Adenosine deaminase (ADA) is suggested to be an important enzyme for modulating the bioactivity of insulin, but its clinical significance in diabetes mellitus (DM) is not yet characterized. We measured the serum level of ADA in healthy controls (C, n=29) and type 2 diabetic patients (n=42). The mean serum level of ADA in C, and type 2 diabetic patients were 29.81±9.15 and. 20.73±8.42 U/L, respectively (P<0.006 vs. C). ADA levels of patients were significantly correlated with HbA1c (r=0.45, p<0.01). Our findings suggest that ADA may play a role in insulin effect and glycamic control. On the other hand, increased activity of ADA in type 2 DM might be a marker for insulin indication. However, further studies are required for the pathogenic role of elevated ADA activity in type 2 DM.

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