19 F NMR Spectroscopy Tagging and Paramagnetic Relaxation Enhancement‐Based Conformation Analysis of Intrinsically Disordered Protein Complexes

ChemBioChem ◽  
2019 ◽  
Vol 21 (5) ◽  
pp. 696-701 ◽  
Author(s):  
Máté Somlyay ◽  
Karin Ledolter ◽  
Manuel Kitzler ◽  
Graham Sandford ◽  
Steven L. Cobb ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Protein Complexes ◽  
Intrinsically Disordered Protein ◽  
Paramagnetic Relaxation ◽  
Paramagnetic Relaxation Enhancement ◽  
Conformation Analysis ◽  
Intrinsically Disordered ◽  
Disordered Protein ◽  
19 F Nmr

scholarly journals Mechanism‐Dependent Modulation of Ultrafast Interfacial Water Dynamics in Intrinsically Disordered Protein Complexes

2019 ◽  
Vol 58 (14) ◽  
pp. 4720-4724 ◽  
Author(s):  
Aritra Chowdhury ◽  
Sergey A. Kovalenko ◽  
Iker Valle Aramburu ◽  
Piau Siong Tan ◽  
Nikolaus P. Ernsting ◽  
...  
Keyword(s):  
Protein Complexes ◽  
Intrinsically Disordered Protein ◽  
Water Dynamics ◽  
Interfacial Water ◽  
Intrinsically Disordered ◽  
Disordered Protein

Self-Assembling Micelles Based on an Intrinsically Disordered Protein Domain

2018 ◽  
Author(s):  
Sarah Klass ◽  
Matthew J. Smith ◽  
Tahoe Fiala ◽  
Jessica Lee ◽  
Anthony Omole ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Fusion Proteins ◽  
Organic Molecules ◽  
Intrinsically Disordered Protein ◽  
Protein Domain ◽  
Enzymatic Cleavage ◽  
Intrinsically Disordered ◽  
Disordered Protein ◽  
Self Assembling ◽  
Protein Tag

Herein, we describe a new series of fusion proteins that have been developed to self-assemble spontaneously into stable micelles that are 27 nm in diameter after enzymatic cleavage of a solubilizing protein tag. The sequences of the proteins are based on a human intrinsically disordered protein, which has been appended with a hydrophobic segment. The micelles were found to form across a broad range of pH, ionic strength, and temperature conditions, with critical micelle concentration (CMC) values below 1 µM being observed in some cases. The reported micelles were found to solubilize hydrophobic metal complexes and organic molecules, suggesting their potential suitability for catalysis and drug delivery applications.

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