Metabolomic, photoprotective, and photosynthetic acclimatory responses to post-flowering drought in sorghum

Climate change is globally affecting rainfall patterns, necessitating the improvement of drought tolerance in crops. Sorghum bicolor is a drought-tolerant cereal capable of producing high yields under water scarcity conditions. Functional stay-green sorghum genotypes can maintain green leaf area and efficient grain filling in terminal post-flowering water deprivation, a period of ~10 weeks. To obtain molecular insights into these characteristics, two drought-tolerant genotypes, BTx642 and RTx430, were grown in control and terminal post-flowering drought field plots in the Central Valley of California. Photosynthetic, photoprotective, water dynamics, and biomass traits were quantified and correlated with metabolomic data collected from leaves, stems, and roots at multiple timepoints during drought. Physiological and metabolomic data was then compared to longitudinal RNA sequencing data collected from these two genotypes. The metabolic response to drought highlights the uniqueness of the post-flowering drought acclimation relative to pre-flowering drought. The functional stay-green genotype BTx642 specifically induced photoprotective responses in post-flowering drought supporting a putative role for photoprotection in the molecular basis of the functional stay-green trait. Specific genes are highlighted that may contribute to post-flowering drought tolerance and that can be targeted in crops to maximize yields under limited water input conditions.

Numerical Investigation on Flood Water Sloshing Influence on Intact and Damaged Stability of Ship

Sloshing affects the intact and damage stability of the ship, which causes variation in dynamic metacentric height (GM) under critical load conditions. The transient flooding soon after the ship damage is analyzed, with floodwater accumulation in large space and causing the ship to suffer huge heel angles. The ship motion and stability changes when sloshing becomes high in partially flooded compartments. Most of the previous researches focus on the motion response of ship alone, hence the variation of stability due to sloshing is to be more critically studied. In the present study, three critical damage locations are identified and flooding through these locations are analyzed using the volume of fluids method. The method focus on finding damage ship motion response, flood water dynamics, and coupled dynamics of both. This is studied using the numerical method FLOW3D. Motion and stability behaviour will be different for different damage locations; hence portside, starboard-side, and aft-end bottom damage cases are considered. The effect of compartment shape and damage location on motion response and stability of the damaged ship is highlighted.

Leaf Traits and Water-Use Characteristics of Impatiens hainanensis, a Limestone-Endemic Plant under Different Altitudes in Dry and Foggy Seasons

The southwestern mountains of Hainan Island are distributed in the southernmost tropical karst landscape of China, and the unique hydrological structure and frequent solifluction droughts lead to double water stress for local plants. Highly heterogeneous water environments affect the water–use characteristics of plants. Plants develop local adaptative mechanisms in response to changes in the external environment. In this paper, hydrogen–oxygen and carbon stable isotope technology, and physiological index measurements were applied to determine the leaf traits, water–use efficiency, and photosynthetic characteristics of Impatiens hainanensis leaves in dry and foggy seasons, hoping to expound the adaptation mechanism of I. hainanensis leaves to the water dynamics in dry and foggy seasons. In dry and foggy seasons (November 2018 to April 2019), the leaves of I. hainanensis at low and medium altitudes have the following combination of traits: larger leaf dry weights, leaf areas, and specific leaf areas; smaller leaf thicknesses and leaf dry matter contents; and higher chlorophyll contents. In comparison, the leaves of I. hainanensis at high altitudes have the following combination of traits: smaller leaf dry weights, leaf areas, and specific leaf areas; larger leaf thicknesses and leaf dry matter contents; and lower chlorophyll contents. The leaves of I. hainanensis can absorb fog water through their leaves. When the leaves are sprayed with distilled water, the water potential is low, the water potential value gradually increases, and the leaves have a higher rate of water absorption. The leaves of I. hainanensis at low and medium altitudes have the following water–use characteristics: high photosynthesis, high transpiration, and low water–use efficiency. At high altitudes, the Pn of I. hainanensis decreases by 8.43% relative to at low altitudes and by 7.84% relative to at middle altitudes; the Tr decreased by 4.21% relative to at low altitudes and by 3.38% relative to at middle altitude; the WUE increased by 16.61% relative to at low altitudes and increased by 40.79% relative to at middle altitudes. The leaves of I. hainanensis at high altitudes have the following water–use characteristics: low photosynthesis, low transpiration, and high water–use efficiency. I. hainanensis develop different physiological mechanisms of water adaptation by weighing the traits of the leaves and their use of light and water to obtain resources during dry and foggy seasons.

Estimation of Stagnosol Hydraulic Properties and Water Flow Using Uni- and Bimodal Porosity Models in Erosion-Affected Hillslope Vineyard Soils

Erosion has been reported as one of the top degradation processes that negatively affect agricultural soils. The study objective was to identify hydropedological factors controlling soil water dynamics in erosion-affected hillslope vineyard soils. The hydropedological study was conducted at identically-managed Jastrebarsko (location I), and Jazbina (II) and (III) sites with Stagnosol soils. Soil Hydraulic Properties (SHP) were estimated on intact soil cores using Evaporation and WP4C methods; soil hydraulic functions were fitted using HYPROP-FIT software. For Apg and Bg/Btg horizons, uni- and bimodal soil hydraulic models could be well fitted to data; although, the bimodal model performed better in particular cases where data indicated non-uniform pore size distribution. With these SHP estimations, a one-year (2020) water flow scenario was simulated using HYDRUS-1D to compare water balance results obtained with uni- and bimodal hydraulic functions. Simulation results revealed relatively similar flux distribution at each hillslope position between the water balance components infiltration, surface runoff, and drainage. However, at the bottom profile at Jastrebarsko, bimodality of the hydraulic functions led to increased drainage. Soil water storage was reduced, and the vertical movement increased due to modified soil water retention curve shapes. Adequate parameterization of SHP is required to capture the hydropedological response of heterogenous erosion-affected soil systems.

Soil moisture observation in a forested headwater catchment: combining a dense cosmic-ray neutron sensor network with roving and hydrogravimetry at the TERENO site Wüstebach

Cosmic Ray Neutron Sensing (CRNS) has become an effective method to measure soil moisture at a horizontal scale of hundreds of meters and a depth of decimeters. Recent studies proposed to operate CRNS in a network with overlapping footprints in order to cover root-zone water dynamics at the small catchment scale, and, at the same time, to represent spatial heterogeneity. In a joint field campaign from September to November 2020 (JFC-2020), five German research institutions deployed 15 CRNS sensors in the 0.4 km2 Wüstebach catchment (Eifel mountains, Germany). The catchment is dominantly forested (but includes a substantial fraction of open vegetation), and features a topographically distinct watershed. In addition to the dense CRNS coverage, the campaign featured a unique combination of additional instruments and techniques: hydro-gravimetry (to detect water storage dynamics also below the root zone); ground-based and, for the first time, airborne CRNS roving; an extensive wireless soil sensor network, supplemented by manual measurements; and six weighable lysimeters. Together with comprehensive data from the long-term local research infrastructure, the published dataset (available at https://doi.org/10.23728/b2share.afb20a34a6ac429ca6b759238d842765) will be a valuable asset in various research contexts: to advance the retrieval of landscape water storage from CRNS, wireless soil sensor networks, or hydrogravimetry; to identify scale-specific combinations of sensors and methods to represent soil moisture variability; to improve the understanding and simulation of land-atmosphere exchange as well as hydrological and hydrogeological processes at the hill-slope and the catchment scale; and to support the retrieval soil water content from airborne and spaceborne remote sensing platforms.

Hydrotropic Root Behavior in Water-saving Cultivation: a High-resolution Monitoring Study of Soil Water Dynamics

PurposeSubsurface irrigation has been confirmed to have high water use efficiency (WUE) due to it irrigating only the crop root zone. This study investigated hydrotropic root behavior when a wet zone was produced around the roots by subsurface irrigation to clarify the dynamics of soil water content in the wet zone caused by water absorption of the growing plant. ResultsWe conducted a feasibility study of a high-resolution soil moisture sensing prototype and gathered data to analyze hydrotropism and plant water absorption activity. We applied signal processing, high pass filtering, and Fast Fourier Transform (FFT) to the acquired high-resolution soil moisture data. The results showed distinct fluctuation of moisture at the boundary area, which indicated plant’s biological rhythm of photosynthetic activities. We also quantified root distribution inside and outside the wet zone and observed the shape of the root system from the cross-section of the wet zone. The results show that hydrotropism restricted most of the roots to the inside of the wet zone. Furthermore, root hydrotropic response is nonuniform for all roots of an individual plant. ConclusionsThe results suggest a new method to study hydrotropic root behavior and plant photosynthetic activities. We assumed a mechanical, push-and-pull model of water dynamics at the wetting front and the root mass accumulated by hydrotropism is an important system parameter. To further evaluate a plant’s hydrotropic performance, it is necessary to use stochastic analysis and/or a non-deterministic approach.