Ectopic expression ofDAZLgene in goat bone marrow-derived mesenchymal stem cells enhances the trans-differentiation to putative germ cells compared to the exogenous treatment of retinoic acid or bone morphogenetic protein 4 signalling molecules

2014 ◽  
Vol 39 (1) ◽  
pp. 74-83 ◽  
Author(s):  
Guangyao Yan ◽  
Yixuan Fan ◽  
Peizhen Li ◽  
YanLi Zhang ◽  
Feng Wang
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Retinoic Acid ◽  
Germ Cells ◽  
Ectopic Expression ◽  
Bone Morphogenetic Protein 4 ◽  
Signalling Molecules ◽  
Morphogenetic Protein ◽  
Exogenous Treatment

scholarly journals MicroRNA-320a Regulates the Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells by Targeting HOXA10

2016 ◽  
Vol 38 (1) ◽  
pp. 40-48 ◽  
Author(s):  
Jianhou Huang ◽  
Yake Meng ◽  
Yan Liu ◽  
Yu Chen ◽  
Haisong Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Ectopic Expression ◽  
In Silico Analysis ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Matrix Mineralization ◽  
Morphogenetic Protein

Background/Aims: Human bone marrow-derived mesenchymal stem cells (hMSCs) are a promising cell source for bone engineering owing to their high potential to differentiate into osteoblasts. The bone morphogenetic protein-inducible gene homeobox a10 (HOXA10) is a critical regulator of osteogenesis. The objective of the present study was to identify microR-NAs (miRNAs) targeting HOXA10 and examine the effects on the osteogenic differentiation of hMSCs. Methods: Based on in silico analysis, HOXA10-targeting miRNAs were selected and their regulatory roles in osteoblast differentiation were investigated. Results: Six HOXA10-targeting miRNAs were identifIed by computational analysis, of which miR-320a was selected for further analysis because it was downregulated during osteogenic induction. Overexpression of miR-320a downregulated HOXA10 and significantly inhibited osteogenesis in hMSCs, as determined by the downregulation of the osteogenic markers Runx2, ALP, and OC and the inhibition of ALP activity and matrix mineralization, whereas miR-320a inhibition had the opposite effects. Furthermore, ectopic expression of HOXA10 (not including 3′-UTR) rescued the effects of miR-320a on osteogenic differentiation. Conclusion: These results suggest that miR-320a acts as a critical regulator of osteogenic differentiation of hMSCs by repressing its target HOXA10.

Distinct roles of retinoic acid and BMP4 pathways in the formation of chicken primordial germ cells and spermatogonial stem cells

2019 ◽  
Vol 10 (11) ◽  
pp. 7152-7163 ◽  
Author(s):  
Qisheng Zuo ◽  
Jing Jin ◽  
Kai Jin ◽  
Changhua Sun ◽  
Jiuzhou Song ◽  
...  
Keyword(s):  
Stem Cells ◽  
Retinoic Acid ◽  
Germ Cell ◽  
Bone Morphogenetic Protein ◽  
Germ Cells ◽  
Primordial Germ Cells ◽  
Cell Formation ◽  
Bone Morphogenetic Protein 4 ◽  
Morphogenetic Protein ◽  
Germ Cell Formation

This study demonstrated different effects of bone morphogenetic protein 4 (BMP4) and retinoic acid (RA) signaling on the induction of germ cell formation in chickens.

Comparison of the efficacy of three concentrations of retinoic acid for transdifferentiation induction in sheep marrow-derived mesenchymal stem cells into male germ cells

Andrologia ◽  
2012 ◽  
Vol 46 (1) ◽  
pp. 24-35 ◽  
Author(s):  
M. Ghasemzadeh-Hasankolaei ◽  
M.B. Eslaminejad ◽  
R. Batavani ◽  
M. Sedighi-Gilani
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Retinoic Acid ◽  
Germ Cells ◽  
Male Germ Cells

Organic and inorganic zinc show similar regulatory effects on the expression of some germ cell specific markers induced in bone marrow mesenchymal stem cells after treatment with retinoic acid

Biologia ◽  
2019 ◽  
Vol 74 (12) ◽  
pp. 1721-1731
Author(s):  
Maryam Ghasemzadeh-Hasankolaei ◽  
Forough Azam Sayahpour ◽  
Mohammad Ghasemzadeh-Hasankolaei ◽  
Mohammad Taghi Ghorbanian ◽  
Mohamadreza B. Eslaminejad
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Retinoic Acid ◽  
Germ Cell ◽  
Marrow Mesenchymal Stem Cells ◽  
Regulatory Effects
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