Temporal Effect of Melatonin Posttreatment on Anoxia/Reoxygenation Injury in H9c2 Cells

2022 ◽  
Author(s):  
Yang Bai ◽  
Yanli Yang ◽  
Boqun Cui ◽  
Duomao Lin ◽  
Zhaoqi Wang ◽  
...  
Keyword(s):  
H9c2 Cells ◽  
Temporal Effect ◽  
Reoxygenation Injury

scholarly journals Pseudosterins A–C, Three 1-Ethyl-3-formyl-β-carbolines from Pseudostellaria heterophylla and Their Cardioprotective Effects

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 5045
Author(s):  
Guo-Bo Xu ◽  
Qin-Feng Zhu ◽  
Zhen Wang ◽  
Chun-Li Zhang ◽  
Xin Yang ◽  
...  
Keyword(s):  
Quantum Chemical ◽  
Functional Food ◽  
Previous Investigation ◽  
Spectroscopic Analysis ◽  
Protective Effects ◽  
H9c2 Cells ◽  
Hypoxic Injury ◽  
Pseudostellaria Heterophylla ◽  
Reoxygenation Injury ◽  
Cardioprotective Effects

Pseudostellaria heterophylla is used in China not only as a functional food but also as an herb to tonify the spleen, enhance immunity, and treat palpitation. Our previous investigation showed that a fraction enriched in glycosides obtained from the roots of P. heterophylla possessed pronounced protective effects on H9c2 cells against CoCl2-induced hypoxic injury. However, the active compounds responsible for the observed effects were still unknown. In the current investigation, pseudosterins A–C (1–3), three new alkaloids with a 1-ethyl-3-formyl-β-carboline skeleton, together with polydatin, have been isolated from the active fraction. Their structures were elucidated on the basis of spectroscopic analysis and quantum chemical calculations. The four compounds showed cardioprotective effects against sodium hydrosulfite-induced hypoxia-reoxygenation injury in H9c2 cells, with the three alkaloids being more potent. This is also the first report of alkaloids with a β-carboline skeleton isolated from P. heterophylla as cardioprotective agents.

scholarly journals Naringin Effectively Protects Cardiomyocytes Against Hypoxia/Reoxygenation Injury

2021 ◽  
Vol 37 (3) ◽  
Author(s):  
Vu Thi Thu ◽  
Ngo Thi Hai Yen
Keyword(s):  
Membrane Potential ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Cell Group ◽  
H9c2 Cell ◽  
H9c2 Cells ◽  
H9c2 Cardiomyocytes ◽  
Reoxygenation Injury ◽  
Hypoxia Reoxygenation

This study was conducted to evaluate the protective effect of Naringin (NAR) on H9C2 cardiomyocytes in hypoxia/reoxygenation (HR) injury in vitro induced by the hypoxia chamber. Methods: H9C2 cells were grown under normal (control) and HR conditions. The viability, cardiolipin content and mitochondrial membrane potential of H9C2 cells in experimental groups were analyzed by using suitable kits. Results: The obtained results showed that the addition of Naringin (16÷160 µM) significantly increased the survival rate of H9C2 cells under HR conditions. In particular, NAR had the highest efficiency in preserving mitochondrial function at concentrations of 80 µM and 160 µM. In HR-exposed H9C2 cell group, the cardiolipin content and mitochondrial membrane potential values of H9C2 cells were decreased sharply with that of control (71,64±1,37% and 68,12±2,78%, p<0,05). Interestingly, mitochondrial cardiolipin contents were signigicantly increased in H9C2 cells post-hypoxic treated wtih NAR at dose of 80 µM 160 µM to 87,76±1,89% and 81,09±1,21%. Additionally, post-hypoxic supplementation of NAR at concentration of 80 µM and 160 µM effectively increased mitochondrial membrane potential values. Conclusion: The obtained results are preliminary data on the effects of NAR in protecting mitochondrial-targeted cardiomyocytes against HR injury.

scholarly journals Knockdown of forkhead box protein P1 alleviates hypoxia reoxygenation injury in H9c2 cells through regulating Pik3ip1/Akt/eNOS and ROS/mPTP pathway

Bioengineered ◽  
2022 ◽  
Vol 13 (1) ◽  
pp. 1320-1334
Author(s):  
Xinming Liu ◽  
Yixing Yang ◽  
Jiawei Song ◽  
Dongjie Li ◽  
Xiaoyan Liu ◽  
...  
Keyword(s):  
H9c2 Cells ◽  
Forkhead Box ◽  
Reoxygenation Injury ◽  
Hypoxia Reoxygenation

scholarly journals Melatonin Attenuates Anoxia/Reoxygenation Injury by Inhibiting Excessive Mitophagy Through the MT2/SIRT3/FoxO3a Signaling Pathway in H9c2 Cells

2020 ◽  
Vol Volume 14 ◽  
pp. 2047-2060 ◽  
Author(s):  
Jinjing Wu ◽  
Yanli Yang ◽  
Yafen Gao ◽  
Zhaoqi Wang ◽  
Jun Ma
Keyword(s):  
Signaling Pathway ◽  
H9c2 Cells ◽  
Reoxygenation Injury

Acetylcholine Attenuates Hypoxia/Reoxygenation Injury by Inducing Mitophagy Through PINK1/Parkin Signal Pathway in H9c2 Cells

2015 ◽  
Vol 231 (5) ◽  
pp. 1171-1181 ◽  
Author(s):  
Lei Sun ◽  
Mei Zhao ◽  
Yang Yang ◽  
Run-Qing Xue ◽  
Xiao-Jiang Yu ◽  
...  
Keyword(s):  
Signal Pathway ◽  
H9c2 Cells ◽  
Reoxygenation Injury ◽  
Hypoxia Reoxygenation

scholarly journals Interplay of microRNA‐503 and N‐acetylcysteine in regulating hypoxia‐reoxygenation injury in cardiomyocyte H9C2 cells

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
Yanjing He ◽  
Yin Cai ◽  
Michael G Irwin ◽  
Zhengyuan Xia
Keyword(s):  
H9c2 Cells ◽  
Reoxygenation Injury ◽  
Hypoxia Reoxygenation
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