scholarly journals Pseudosterins A–C, Three 1-Ethyl-3-formyl-β-carbolines from Pseudostellaria heterophylla and Their Cardioprotective Effects

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 5045
Author(s):  
Guo-Bo Xu ◽  
Qin-Feng Zhu ◽  
Zhen Wang ◽  
Chun-Li Zhang ◽  
Xin Yang ◽  
...  
Keyword(s):  
Quantum Chemical ◽  
Functional Food ◽  
Previous Investigation ◽  
Spectroscopic Analysis ◽  
Protective Effects ◽  
H9c2 Cells ◽  
Hypoxic Injury ◽  
Pseudostellaria Heterophylla ◽  
Reoxygenation Injury ◽  
Cardioprotective Effects

Pseudostellaria heterophylla is used in China not only as a functional food but also as an herb to tonify the spleen, enhance immunity, and treat palpitation. Our previous investigation showed that a fraction enriched in glycosides obtained from the roots of P. heterophylla possessed pronounced protective effects on H9c2 cells against CoCl2-induced hypoxic injury. However, the active compounds responsible for the observed effects were still unknown. In the current investigation, pseudosterins A–C (1–3), three new alkaloids with a 1-ethyl-3-formyl-β-carboline skeleton, together with polydatin, have been isolated from the active fraction. Their structures were elucidated on the basis of spectroscopic analysis and quantum chemical calculations. The four compounds showed cardioprotective effects against sodium hydrosulfite-induced hypoxia-reoxygenation injury in H9c2 cells, with the three alkaloids being more potent. This is also the first report of alkaloids with a β-carboline skeleton isolated from P. heterophylla as cardioprotective agents.

scholarly journals Two Nitrogen-containing Compounds from Pseudostellaria heterophylla

2018 ◽  
Vol 13 (3) ◽  
pp. 1934578X1801300
Author(s):  
Xiang-Ming Liao ◽  
Xun He ◽  
Guo-Bo Xu ◽  
Zhen Wang ◽  
Jing Li ◽  
...  
Keyword(s):  
Absolute Configuration ◽  
Quantum Chemical ◽  
Model Compound ◽  
Spectroscopic Methods ◽  
Protective Effects ◽  
Myocardial Cells ◽  
Etoh Extract ◽  
Pseudostellaria Heterophylla ◽  
The Absolute ◽  
Nitrogen Containing Compounds

Two new nitrogen-containing compounds, pseudosteheterins A (1) and B (2), were isolated from the glycosides-enriched cardioprotective fraction of the 70% EtOH extract of the roots of Pseudostellaria heterophylla. Their structures were elucidated by spectroscopic methods, and the absolute configuration of 1 was established by quantum chemical TDDFT calculation of the ECD spectrum of its model compound. Pseudosteheterin B showed significant protective effects in H9c2 myocardial cells against CoCl2-induced injury.

Protective effects of fractions from Pseudostellaria heterophylla against cobalt chloride-induced hypoxic injury in H9c2 cell

2013 ◽  
Vol 147 (2) ◽  
pp. 540-545 ◽  
Author(s):  
Zhen Wang ◽  
Shang-Gao Liao ◽  
Yan He ◽  
Jing Li ◽  
Rui-Feng Zhong ◽  
...  
Keyword(s):  
Cobalt Chloride ◽  
H9c2 Cell ◽  
Protective Effects ◽  
Hypoxic Injury ◽  
Pseudostellaria Heterophylla

scholarly journals Total Flavonoids from Carya cathayensis Sarg. Leaves Alleviate H9c2 Cells Hypoxia/Reoxygenation Injury via Effects on miR-21 Expression, PTEN/Akt, and the Bcl-2/Bax Pathway

2018 ◽  
Vol 2018 ◽  
pp. 1-13 ◽  
Author(s):  
Ruibin Jiang ◽  
Yan Guo ◽  
Nipi Chen ◽  
Chengxian Gao ◽  
Zhishan Ding ◽  
...  
Keyword(s):  
Akt Signaling ◽  
Total Flavonoids ◽  
Protective Effects ◽  
H9c2 Cells ◽  
H9c2 Cardiomyocytes ◽  
Reoxygenation Injury ◽  
Carya Cathayensis ◽  
Carya Cathayensis Sarg ◽  
Signaling Activity ◽  
Hypoxia Reoxygenation

This study aimed to investigate whether the total flavonoids (TFs) from Carya cathayensis Sarg. leaves alleviate hypoxia/reoxygenation (H/R) injury in H9c2 cardiomyocytes and to explore potential mechanisms. H9c2 cells pretreated with TFs for 24h were exposed to H/R treatment. The results indicated that TFs significantly alleviate H/R injury, which include inhibiting apoptosis and enhancing antioxidant capacity. The protective effects of TFs resulted in higher expression of miR-21 in H/R-induced H9c2 cells than that of controls, which in turn upregulated Akt signaling activity via suppressing the expression of PTEN together with decreasing the ratio of Bax/Bcl-2, caspase3, and cleaved-caspase3 expression in H/R-induced H9c2 cells. Conversely, blocking miR-21 expression with miR-21 inhibitor effectively suppressed the protective effects of TFs against H/R-induced injury. Our study suggests that TFs can decrease cell apoptosis, which may be mediated by altering the expression of miR-21, PTEN/Akt, and Bcl/Bax.

scholarly journals The co-treatment of rosuvastatin with dapagliflozin synergistically inhibited apoptosis via activating the PI3K/AKt/mTOR signaling pathway in myocardial ischemia/reperfusion injury rats

Open Medicine ◽  
2020 ◽  
Vol 16 (1) ◽  
pp. 047-057
Author(s):  
Lei Gong ◽  
Xuyang Wang ◽  
Jinyu Pan ◽  
Mingjun Zhang ◽  
Dian Liu ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Signaling Pathway ◽  
Ischemia Reperfusion ◽  
Left Ventricular Pressure ◽  
Left Ventricular ◽  
Mtor Signaling ◽  
Maximal Rate ◽  
H9c2 Cells ◽  
Mtor Signaling Pathway ◽  
Cardioprotective Effects

AbstractObjectiveThe purpose of the present study was to evaluate the role of co-treatment of rosuvastatin (RSV) and dapagliflozin (DGZ) preconditioning in myocardium ischemia/reperfusion (I/R) injury and to further investigate the underlying mechanism.MethodsSprague-Dawley (SD) rats (n = 25) were divided into five groups randomly: (1) Sham, (2) I/R, (3) I/R + RSV (10 mg/kg), (4) IR + DGZ (1 mg/kg), and (5) I/R + RSV (10 mg/kg) + DGZ (1 mg/kg). The I/R model was induced with 30 min of left anterior descending occlusion followed by 120 min of reperfusion.ResultsIn vivo pretreatment with RSV and DGZ, respectively, showed a significant reduction of infarction size, a significant increase in the levels of left ventricular systolic pressure, and maximal rate increase in left ventricular pressure (+dp/dtmax), decrease in the levels of left ventricular end-diastolic pressure (LVEDP), maximal rate of decrease of left ventricular pressure (−dp/dtmax) and activity of cardiac enzymes of creatine kinase (CK), creatine kinase MB isoenzymes (CK-MB), and hyper-tensive cardiac troponin I compared with the I/R group. H9C2 cells were exposed to hypoxia/reoxygenation to simulate an I/R model. In vitro administration of 25 µM RSV and 50 µM DGZ significantly enhanced cell viability, upregulated the expression levels of p-PI3K, p-Akt, p-mTOR, and Bcl-2, whereas it downregulated cleaved-caspase3, Bax. TUNEL assay indicated that pretreatment with RSV and DGZ decreased the apoptosis of H9C2 cells.ConclusionThe combination of RSV and DGZ significantly enhances the cardioprotective effects compared with RSV or DGZ alone. RSV and DGZ have the potential cardioprotective effects against I/R injury by activating the PI3K/AKt/mTOR signaling pathway.

Two new defensive constituents from potato tubers (Solanum tuberosum)

2017 ◽  
Vol 72 (6) ◽  
pp. 393-396
Author(s):  
Liangyan Liu ◽  
Jun Han ◽  
Yong Shen
Keyword(s):  
Solanum Tuberosum ◽  
Late Blight ◽  
Quantum Chemical ◽  
Spectroscopic Analysis ◽  
2D Nmr ◽  
Moderate Activity ◽  
Potato Tubers ◽  
Mycelia Growth ◽  
Late Blight Disease ◽  
Blight Disease

AbstractTwo new defensive constituents, solatuberenol A (1) and 3-O-β-d-glucopyranosyl stigmasta-5(6),24(28)-diene (2), were isolated from the potato tubers (Solanum tuberosum) infected with late blight disease. Their structures were identified by extensive spectroscopic analysis, including HRMS, IR, UV, 1D/2D NMR, ECD and quantum chemical calculations. Compounds 1 and 2 showed moderate activity against Phytophthora infestans with mycelia-growth inhibition of 30.1% and 52.4%, respectively, at the concentration of 500 ppm.

scholarly journals CTRP9 Mediates Protective Effects in Cardiomyocytes via AMPK- and Adiponectin Receptor-Mediated Induction of Anti-Oxidant Response

Cells ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 1229
Author(s):  
Bernd Niemann ◽  
Ling Li ◽  
Dorothee Siegler ◽  
Benedikt H. Siegler ◽  
Fabienne Knapp ◽  
...  
Keyword(s):  
Transcriptional Activation ◽  
Direct Interaction ◽  
Left Ventricular ◽  
Cardiac Cells ◽  
Adiponectin Receptor ◽  
Protective Effects ◽  
Necrosis Factor Alpha ◽  
Anti Oxidant ◽  
Cardioprotective Effects ◽  
The Right

The C1q/tumor necrosis factor-alpha-related protein 9 (CTRP9) has been reported to exert cardioprotective effects, but its role in the right ventricle (RV) remains unclear. To investigate the role of CTRP9 in RV hypertrophy and failure, we performed pulmonary artery banding in weanling rats to induce compensatory RV hypertrophy seven weeks after surgery and RV failure 22 weeks after surgery. CTRP9 expression, signal transduction and mechanisms involved in protective CTRP9 effects were analyzed in rat and human RV tissue and cardiac cells. We demonstrate that CTRP9 was induced during compensatory RV hypertrophy but almost lost at the stage of RV failure. RV but not left ventricular (LV) cardiomyocytes or RV endothelial cells demonstrated increased intracellular reactive oxygen species (ROS) and apoptosis activation at this stage. Exogenous CTRP9 induced AMP-activated protein kinase (AMPK)-dependent transcriptional activation of the anti-oxidant thioredoxin-1 (Trx1) and superoxide dismutase-2 (SOD2) and reduced phenylephrine-induced ROS. Combined knockdown of adiponectin receptor-1 (AdipoR1) and AdipoR2 or knockdown of calreticulin attenuated CTRP9-mediated anti-oxidant effects. Immunoprecipitation showed an interaction of AdipoR1 with AdipoR2 and the co-receptor T-cadherin, but no direct interaction with calreticulin. Thus, CTRP9 mediates cardioprotective effects through inhibition of ROS production induced by pro-hypertrophic agents via AMPK-mediated activation of anti-oxidant enzymes.

scholarly journals Dexrazoxane preferentially mitigates doxorubicin cardiotoxicity in female children with sarcoma

Open Heart ◽  
2019 ◽  
Vol 6 (1) ◽  
pp. e001025 ◽  
Author(s):  
Hari K Narayan ◽  
Mary E Putt ◽  
Nikitha Kosaraju ◽  
Alejandro Paz ◽  
Shivani Bhatt ◽  
...  
Keyword(s):  
Circumferential Strain ◽  
Wall Stress ◽  
Left Ventricular ◽  
High Dose ◽  
Protective Effects ◽  
Cavity Size ◽  
Linear Regression Models ◽  
Cardioprotective Effects ◽  
And Function

ObjectiveWe sought to determine how sex and dexrazoxane therapy influence cardiac remodelling in children with sarcoma receiving high-dose doxorubicin.MethodsIn a retrospective cohort of 85 children with sarcoma receiving high-dose doxorubicin, echocardiography measures prior to, early after (within 6 months of doxorubicin completion) and 1 – 2 years after doxorubicin completion were quantified. At each follow-up visit, multivariable, propensity-adjusted linear regression models evaluated dexrazoxane’s effects on changes in left ventricular (LV) shortening fraction (SF), structure, strain and wall stress for subgroups divided by sex. Likelihood ratio tests assessed the interaction between sex and dexrazoxane in determining these changes.ResultsEarly after doxorubicin completion, males not treated with dexrazoxane (n = 15) developed increased cavity size and diminished circumferential strain; females (n = 8) developed diminished SF and strain indices, and increased cavity size and wall stress. With dexrazoxane, males (n = 33) demonstrated less deterioration in circumferential strain by 3.4% (95% CI 0.01 to 6.8), and females (n = 29) demonstrated less reduction in SF by 5.7% (95% CI 2.1 to 9.3), and had mitigation of increases in cavity size and wall stress. In interaction analyses, females had greater protection with dexrazoxane with regard to SF (p = 0.019) and cavity size in diastole (p = 0.002) and systole (p ≤ 0.001). These findings largely persisted 1 – 2 years after doxorubicin therapy.ConclusionsEarly, sustained alterations in LV structure and function occur in children with sarcoma after high-dose doxorubicin, with adverse changes and protective effects of dexrazoxane more pronounced in females as compared with males. Dexrazoxane may have sex-specific cardioprotective effects.

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