scholarly journals Mapping Surface Charge of Individual Microdomains with Scanning Ion Conductance Microscopy

2018 ◽  
Vol 5 (20) ◽  
pp. 2986-2990 ◽  
Author(s):  
Cheng Zhu ◽  
Lushan Zhou ◽  
Myunghoon Choi ◽  
Lane A. Baker
Keyword(s):  
Surface Charge ◽  
Ion Conductance ◽  
Scanning Ion Conductance Microscopy

scholarly journals Scanning Ion Conductance Microscopy: Surface Charge Effects on Electroosmotic Flow Delivery from a Nanopipette

2021 ◽  
Author(s):  
James Teahan ◽  
David Perry ◽  
Baoping Chen ◽  
Ian J. McPherson ◽  
Gabriel N. Meloni ◽  
...  
Keyword(s):  
Surface Charge ◽  
Electroosmotic Flow ◽  
Ion Conductance ◽  
Charge Effects ◽  
Scanning Ion Conductance Microscopy

Gauging surface charge distribution of live cell membrane by ionic current change using scanning ion conductance microscopy

Nanoscale ◽  
2021 ◽  
Author(s):  
Feng Chen ◽  
Jin He ◽  
Prakash Manandhar ◽  
Yizi Yang ◽  
Peidang Liu ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Surface Charge ◽  
Charge Distribution ◽  
Ionic Current ◽  
Live Cells ◽  
Ion Conductance ◽  
Physiological Conditions ◽  
Scanning Ion Conductance Microscopy ◽  
Surface Charge Distribution ◽  
Current Change

The distribution of surface charge and potential of cell membrane plays an indispensable role in cellular activities. However, probing surface charge of live cells in physiological conditions, until recently, remains...

scholarly journals Fast Nanoscale Surface Charge Mapping with Pulsed-Potential Scanning Ion Conductance Microscopy

2016 ◽  
Vol 88 (22) ◽  
pp. 10854-10859 ◽  
Author(s):  
Ashley Page ◽  
David Perry ◽  
Philip Young ◽  
Daniel Mitchell ◽  
Bruno G. Frenguelli ◽  
...  
Keyword(s):  
Surface Charge ◽  
Ion Conductance ◽  
Scanning Ion Conductance Microscopy

scholarly journals Scanning ion conductance microscopy reveals differences in the ionic environments of gram positive and negative bacteria

2020 ◽  
Author(s):  
Kelsey Cremin ◽  
Bryn Jones ◽  
James Teahan ◽  
Gabriel N. Meloni ◽  
David Perry ◽  
...  
Keyword(s):  
Cell Wall ◽  
Surface Charge ◽  
Wall Structure ◽  
Current Response ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Fem Model ◽  
Ion Conductance ◽  
E Coli ◽  
Scanning Ion Conductance Microscopy

AbstractThis paper reports on the use of scanning ion conductance microscopy (SICM) to locally map the ionic properties and charge environment of two live bacterial strains: the gramnegative Escherichia coli and the gram-positive Bacillus subtilis. SICM results find heterogeneities across the bacterial surface, and significant differences among the grampositive and -negative bacteria. The bioelectrical environment of the B. subtilis was found to be considerably more negatively charged compared to E. coli. SICM measurements, fitted to a simplified finite element method (FEM) model, revealed surface charge values of −80 to −140 mC m−2 for the gram-negative E. coli. The gram-positive B. subtilis show a much higher conductivity around the cell wall, and surface charge values between −350 and −450 mC m−2 were found using the same simplified model. SICM was also able to detect regions of high negative charge near B. subtilis, not detected in the topographical SICM response and attributed to extracellular polymeric substance. To further explore how the B. subtilis cell wall structure can influence the SICM current response, a more comprehensive FEM model, accounting for the physical properties of the gram-positive cell wall, was developed. The new model provides a more realistic description of the cell wall and allowed investigation of the relation between its key properties and SICM currents, building foundations to further investigate and improve understanding of the gram-positive cellular microenvironment.Abstract Figure

scholarly journals Multifunctional scanning ion conductance microscopy

2017 ◽  
Vol 473 (2200) ◽  
pp. 20160889 ◽  
Author(s):  
Ashley Page ◽  
David Perry ◽  
Patrick R. Unwin
Keyword(s):  
Surface Charge ◽  
Single Cells ◽  
Three Dimensional ◽  
Ion Conductance ◽  
Scanning Ion Conductance Microscopy ◽  
New Methods ◽  
Single Probe ◽  
Advanced Control ◽  
Opening Up ◽  
Electrochemical Microscopy

Scanning ion conductance microscopy (SICM) is a nanopipette-based technique that has traditionally been used to image topography or to deliver species to an interface, particularly in a biological setting. This article highlights the recent blossoming of SICM into a technique with a much greater diversity of applications and capability that can be used either standalone, with advanced control (potential–time) functions, or in tandem with other methods. SICM can be used to elucidate functional information about interfaces, such as surface charge density or electrochemical activity (ion fluxes). Using a multi-barrel probe format, SICM-related techniques can be employed to deposit nanoscale three-dimensional structures and further functionality is realized when SICM is combined with scanning electrochemical microscopy (SECM), with simultaneous measurements from a single probe opening up considerable prospects for multifunctional imaging. SICM studies are greatly enhanced by finite-element method modelling for quantitative treatment of issues such as resolution, surface charge and (tip) geometry effects. SICM is particularly applicable to the study of living systems, notably single cells, although applications extend to materials characterization and to new methods of printing and nanofabrication. A more thorough understanding of the electrochemical principles and properties of SICM provides a foundation for significant applications of SICM in electrochemistry and interfacial science.

scholarly journals Surface Charge Mapping Based on Scanning Ion Conductance Microscopy

2010 ◽  
Vol 98 (3) ◽  
pp. 395a
Author(s):  
Louis Burkinshaw ◽  
Richard Clarke ◽  
Pavel Novak ◽  
Yuri Korchev ◽  
David Klenerman
Keyword(s):  
Surface Charge ◽  
Ion Conductance ◽  
Scanning Ion Conductance Microscopy

Surface Charge Measurements with Scanning Ion Conductance Microscopy Provide Insights into Nitrous Acid Speciation at the Kaolin Mineral–Air Interface

2021 ◽  
Vol 55 (18) ◽  
pp. 12233-12242
Author(s):  
Cheng Zhu ◽  
Gargi Jagdale ◽  
Adrien Gandolfo ◽  
Kristen Alanis ◽  
Rebecca Abney ◽  
...  
Keyword(s):  
Surface Charge ◽  
Nitrous Acid ◽  
Ion Conductance ◽  
Scanning Ion Conductance Microscopy ◽  
Air Interface

Prior knowledge based fast imaging for scanning ion conductance microscopy

2013 ◽  
Author(s):  
Peng Li ◽  
Changlin Zhang ◽  
Lianqing Liu ◽  
Yuechao Wang ◽  
Ning Xi ◽  
...  
Keyword(s):  
Prior Knowledge ◽  
Fast Imaging ◽  
Ion Conductance ◽  
Scanning Ion Conductance Microscopy ◽  
Knowledge Based

scholarly journals Cell stiffness and ROS level alterations in living neurons mediated by β-amyloid oligomers measured by scanning ion-conductance microscopy.

2021 ◽  
Vol 27 (S1) ◽  
pp. 500-502
Author(s):  
Oleg Suchalko ◽  
Roman Timoshenko ◽  
Alexander Vaneev ◽  
Vasilii Kolmogorov ◽  
Nikita Savin ◽  
...  
Keyword(s):  
Cell Stiffness ◽  
Ion Conductance ◽  
Scanning Ion Conductance Microscopy ◽  
Amyloid Oligomers ◽  
Β Amyloid ◽  
Living Neurons

scholarly journals Nanoscale Cell Membrane Fluctuations Measured by Scanning Ion Conductance Microscopy

2013 ◽  
Vol 104 (2) ◽  
pp. 317a
Author(s):  
Yusuke Mizutani ◽  
Zen Ishikura ◽  
Myung-Hoon Choi ◽  
Sang-Joon Cho ◽  
Takaharu Okajima
Keyword(s):  
Cell Membrane ◽  
Ion Conductance ◽  
Scanning Ion Conductance Microscopy ◽  
Membrane Fluctuations
Sign in / Sign up

Export Citation Format

Share Document