Bifunctional Nanoprobes Used for Label‐Free Determination of Cardiac Troponin I

This work demonstrates the quantitative assay of cardiac Troponin I (cTnI), one of the key biomarkers for acute cardiovascular diseases (the leading cause of death worldwide) using the fluorescence-based sandwich immune reaction. Surface plasmon coupled emission (SPCE) produced by non-radiative coupling of dye molecules with surface plasmons being excitable via the reverse Kretschmann format is exploited for fluorescence-based sandwich immunoassay for quantitative detection of cTnI. The SPCE fluorescence chip utilizes the gold (2 nm)-silver (50 nm) bimetallic thin film, with which molecules of the dye Alexa 488 (conjugated with detection antibodies) make a near field coupling with the plasmonic film for SPCE. The experimental results find that the SPCE greatly improves the sensitivity via enhancing the fluorescence signal (up to 50-fold) while suppressing the photo-bleaching, permitting markedly enhanced signal-to-noise ratio. The limit of detection of 21.2 ag mL−1 (atto-gram mL−1) is obtained, the lowest ever reported to date amid those achieved by optical technologies such as luminescence and label-free optical sensing techniques. The features discovered such as ultrahigh sensitivity may prompt the presented technologies to be applied for early diagnosis of cTnI in blood, particularly for emergency medical centers overloaded with patients with acute myocardial infarction who would suffer from time-delayed diagnosis due to insufficient assay device sensitivity.

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In Situ Construction of Oriented Pt‐PANI Needle‐Like Nanoarrays‐Based Label‐Free Aptasensor for Ultrafast and Ultrasensitive Recognition of Cardiac Troponin I

Advanced Materials Interfaces ◽

10.1002/admi.202101543 ◽

2021 ◽

pp. 2101543

Author(s):

Wei Zhang ◽

Tao Liu ◽

Yuansheng Shan ◽

Bailing Hou ◽

Zhenyu Chu ◽

...

Keyword(s):

Cardiac Troponin ◽

Troponin I ◽

Cardiac Troponin I ◽

Label Free

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MIPs-graphene nanoplatelets-MWCNTs modified glassy carbon electrode for the determination of cardiac troponin I

Analytical Biochemistry ◽

10.1016/j.ab.2016.12.018 ◽

2017 ◽

Vol 520 ◽

pp. 9-15 ◽

Cited By ~ 23

Author(s):

Ya Ma ◽

Xiao-Lei Shen ◽

Hai-Shui Wang ◽

Jia Tao ◽

Jian-Zhi Huang ◽

...

Keyword(s):

Glassy Carbon Electrode ◽

Glassy Carbon ◽

Cardiac Troponin ◽

Troponin I ◽

Graphene Nanoplatelets ◽

Cardiac Troponin I ◽

Carbon Electrode ◽

Modified Glassy Carbon Electrode

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Determination of high sensitive cardiac troponin I 99th percentile upper reference limits in a healthy Pakistani population

Pakistan Journal of Medical Sciences ◽

10.12669/pjms.36.6.2328 ◽

2020 ◽

Vol 36 (6) ◽

Author(s):

Kulsoom Bahadur ◽

Aamir Ijaz ◽

Momin Salahuddin ◽

Aftab Alam

Keyword(s):

Cardiac Troponin ◽

Troponin I ◽

Laboratory Data ◽

Cardiac Troponin I ◽

Medical Institute ◽

Pakistani Population ◽

Reference Limits ◽

Males And Females ◽

Gender Specific

Objective: This study aims to establish the 99th percentile upper reference limits of high sensitive cardiac troponin I in a healthy Pakistani population. Methods: It was an Observational cohort study carried out in Department of Chemical Pathology and Endocrinology Rehman Medical Institute Peshawar, over the period of one year (January 2019- December 2019). Total 299 cardio-healthy males and females were interviewed and taken past medical history. Based on history, clinical examination, echocardiogram and laboratory data including results of estimated glomerular filtration rate (eGFR) and N-terminal pro-B-type natriureteric peptide (NT-proBNP), subjects with possible subclinical diseases were excluded. High Sensitive Cardiac Troponin I (hs-cTtrop I) was analysed on Abbot ARCHITECT STAT ci8200 using chemiluminescent immunoassay technique. The 99th percentile upper reference limit (URL) of hs-cTtrop I was determined using a non-parametric statistic, while gender specific results were compared. Results: In this study, 178 males (59.5%) and 121 females (40.5%) were included. The median Interquartile ranges (IQR) of age was 57 (11.6) for males and 56 (13) for females. The 99th percentile URL hs-cTtrop I was found to be 33.9 ng/L, while gender specific values were 38.41ng/L and 15.73ng/L for males and females, respectively (p= 0.0045). Conclusion: High sensitivity cardiac troponin I 99th percentile URL in our study population was found to be 33.9 ng/L with gender specific values being 38.41 ng/L and 15.73ng/L for males and females respectively. Troponin I in males was substantially high in comparison with females. doi: https://doi.org/10.12669/pjms.36.6.2328 How to cite this:Bahadur K, Ijaz A, Salahuddin M, Alam A. Determination of high sensitive cardiac troponin I 99th percentile upper reference limits in a healthy Pakistani population. Pak J Med Sci. 2020;36(6):---------. doi: https://doi.org/10.12669/pjms.36.6.2328 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Electrochemical immunosensor for determination of cardiac troponin I using two-dimensional metal-organic framework/Fe3O4–COOH nanosheet composites loaded with thionine and pCTAB/DES modified electrode

Talanta ◽

10.1016/j.talanta.2021.122911 ◽

2021 ◽

pp. 122911

Author(s):

Anita Ahmadi ◽

Seyyed Mehdi Khoshfetrat ◽

Zahra Mirzaeizadeh ◽

Shima Kabiri ◽

Javad Rezaie ◽

...

Keyword(s):

Modified Electrode ◽

Cardiac Troponin ◽

Troponin I ◽

Metal Organic Framework ◽

Electrochemical Immunosensor ◽

Cardiac Troponin I ◽

Two Dimensional ◽

Metal Organic ◽

Organic Framework

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Long-Range Surface Plasmon-Polariton Waveguide Biosensors for Human Cardiac Troponin I Detection

Sensors ◽

10.3390/s19030631 ◽

2019 ◽

Vol 19 (3) ◽

pp. 631 ◽

Cited By ~ 3

Author(s):

Oleksiy Krupin ◽

Pierre Berini

Keyword(s):

Long Range ◽

Surface Plasmon ◽

Cardiac Troponin ◽

Surface Plasmon Polariton ◽

Troponin I ◽

Biological Marker ◽

Cardiac Troponin I ◽

Label Free ◽

Label Free Detection ◽

Plasmon Polariton

Straight long-range surface plasmon-polariton (LRSPP) waveguides as biosensors for label-free detection are discussed. The sensors consist of 5-μm-wide 35-nm-thick gold stripes embedded in a low-index optical-grade fluoropolymer (CYTOPTM) with fluidic channels etched to the Au surface of the stripes. This work demonstrates the application of the LRSPP biosensors for the detection of human cardiac troponin I (cTnI) protein. cTnI is a biological marker for acute myocardial infarction (AMI), often referred to as a heart attack, which can be diagnosed by elevated levels of cTnI in patient blood. Direct and sandwich assays were developed and demonstrated over the concentration range from 1 to 1000 ng/mL, yielding detection limits of 430 pg/mL for the direct assay and 28 pg/mL for the sandwich assay (1 standard deviation), the latter being physiologically relevant to the early detection or onset of AMI. In addition, a novel approach for data analysis is proposed, where the analyte response is normalized to the response of the antibody layer.

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