ChemInform Abstract: SYNTHESIS OF 4,4-DITRITIO-(+)-NICOTINE: COMPARATIVE BINDING AND DISTRIBUTION STUDIES WITH NATURAL ENANTIOMER

Daunomycin, an antibiotic used in the clinical management of acute leukemia, produces a delayed, lethal cardiac toxicity. The lethality is dose and schedule dependent; histopathologic changes induced by the drug have been described in heart, lung, and kidney from hamsters in both single and multiple dose studies. Mice given a single intravenous dose of daunomycin (10 mg/kg) die 6-7 days later. Drug distribution studies indicate that the rodents excrete most of a single dose of the drug as daunomycin and metabolite within 48 hours after dosage (M. A. Asbell, personal communication).Myocardium from the ventricles of 6 moribund BDF1 mice which had received a single intravenous dose of daunomycin (10 mg/kg), and from controls dosed with physiologic saline, was fixed in glutaraldehyde and prepared for electron microscopy.

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A Freeze Shadow Technique for the Preparation of Soft Paint Latexes for Electron Microscopy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100079097 ◽

1977 ◽

Vol 35 ◽

pp. 314-315

Author(s):

Earl R. Walter ◽

Glen H. Bryant

Keyword(s):

Sample Preparation ◽

High Vacuum ◽

Vacuum System ◽

Latex Particles ◽

New Methods ◽

Diffusion Pump ◽

Film Forming ◽

Routine Basis ◽

Distribution Studies ◽

Preparation Techniques

With the development of soft, film forming latexes for use in paints and other coatings applications, it became desirable to develop new methods of sample preparation for latex particle size distribution studies with the electron microscope. Conventional latex sample preparation techniques were inadequate due to the pronounced tendency of these new soft latex particles to distort, flatten and fuse on the substrate when they dried. In order to avoid these complications and obtain electron micrographs of undistorted latex particles of soft resins, a freeze-dry, cold shadowing technique was developed. The method has now been used in our laboratory on a routine basis for several years.The cold shadowing is done in a specially constructed vacuum system, having a conventional mechanical fore pump and oil diffusion pump supplying vacuum. The system incorporates bellows type high vacuum valves to permit a prepump cycle and opening of the shadowing chamber without shutting down the oil diffusion pump. A baffeled sorption trap isolates the shadowing chamber from the pumps.

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Antileishmanial Activity, Pharmacokinetics and Tissue Distribution Studies of Mannose Grafted Piperine Lipid Nano Spheres

Planta Medica ◽

10.1055/s-0030-1251875 ◽

2010 ◽

Vol 76 (05) ◽

Author(s):

PR Veerareddy ◽

V Vobalaboina ◽

N Ali

Keyword(s):

Tissue Distribution ◽

Antileishmanial Activity ◽

Distribution Studies

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Effects of Preloading of Stannous Compounds on the Distribution of 99mTc-Pertechnetate

Nuklearmedizin ◽

10.1055/s-0037-1620602 ◽

1977 ◽

Vol 16 (01) ◽

pp. 26-29 ◽

Cited By ~ 1

Author(s):

D. D. Greenberg ◽

P. Som ◽

G. E. Meinken ◽

D. F. Sacker ◽

H. L. Atkins ◽

...

Keyword(s):

Blood Cell ◽

Red Blood Cell ◽

Plasma Ratio ◽

99Mtc Pertechnetate ◽

Time Period ◽

Stannous Ion ◽

Distribution Studies

Summary 99mTc-pertechnetate distribution studies were performed in rabbits and mice following pretreatment between 5—336 hours with various routinely used stannous complexes (HSA, MAA, GHT, DTPA, PYPs) containing different amounts of Sn++ (0.17 —15.0 μ mg/kg). Beyond a concentration of 0.26 mg/kg of Sn++ an alteration in 99mTc-pertechnetate distribution was observed. The red blood cell was found to be the most prominent target. An in-vivo reduction of 99mTc-pertechnetate apparently occurred by the presence of stannous ion within the red blood cell. Preloading time period between 5—24 hours did not alter the uptake of RBC/plasma ratio. Beyond that period it decreased slowly and still persisted up to 2 weeks following pretreatment. RBC/ plasma ratio of 99mTcO4 - increased with increased Sn++ content of various commercially available pharmaceutical kits.

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99mTc-Sn-Pyrophosphate Complexes, an Investigation of its Possible Structures

Nuklearmedizin ◽

10.1055/s-0038-1624863 ◽

1974 ◽

Vol 13 (03) ◽

pp. 252-257 ◽

Cited By ~ 1

Author(s):

K. Rörvik - Schümichen ◽

G. Hoffmann ◽

C. Schümichen

Keyword(s):

In Vivo Distribution ◽

Low Concentrations ◽

Distribution Studies

SummaryAt least two different 99mTc-Sn-pyrophosphate complexes are formed, as it is shown by comparative in vivo distribution studies: A 2 : 2 Sn : pyrophosphate complex is predominant at higher concentrations. Only this complex shows bone seeking properties. A 2 : 1 Sn : pyrophosphate complex exists only at low concentrations. This complex shows no deposition in bone but in the kidneys. Which complex is predominant depends on the pyrophosphate concentration in the equilibrium. Both complexes are rapidly excreted by the kidneys.

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