ChemInform Abstract: A Photolabile Disaccharide-C-glycoside as an Affinity Reagent for IgA X24.

ChemInform ◽  
1989 ◽  
Vol 20 (33) ◽  
Author(s):  
C.-S. KUHN ◽  
C. P. J. GLAUDEMANS ◽  
J. LEHMANN
Keyword(s):  
Affinity Reagent

scholarly journals Expedient Synthesis of a Modular Phosphate Affinity Reagent

2010 ◽  
Vol 21 (6) ◽  
pp. 1010-1013 ◽  
Author(s):  
Nicolas P. Tilmans ◽  
Casey J. Krusemark ◽  
Pehr A. B. Harbury
Keyword(s):  
Affinity Reagent

Inhibitors of NF-ϰB Signaling: Design and Synthesis of a Biotinylated Isopanepoxydone Affinity Reagent.

ChemInform ◽  
2003 ◽  
Vol 34 (12) ◽  
Author(s):  
J. Brad Shotwell ◽  
Brian Koh ◽  
Hui Won Choi ◽  
John L. Wood ◽  
Craig M. Crews
Keyword(s):  
Design And Synthesis ◽  
Affinity Reagent

scholarly journals Transcription factor MBF-I interacts with metal regulatory elements of higher eucaryotic metallothionein genes.

1989 ◽  
Vol 9 (12) ◽  
pp. 5315-5323 ◽  
Author(s):  
J Imbert ◽  
M Zafarullah ◽  
V C Culotta ◽  
L Gedamu ◽  
D Hamer
Keyword(s):  
Gene Transcription ◽  
Regulatory Elements ◽  
Gene Promoters ◽  
Direct Role ◽  
Transcription In Vitro ◽  
Mouse Cells ◽  
Affinity Reagent

Metallothionein (MT) gene promoters in higher eucaryotes contain multiple metal regulatory elements (MREs) that are responsible for the metal induction of MT gene transcription. We identified and purified to near homogeneity a 74-kilodalton mouse nuclear protein that specifically binds to certain MRE sequences. This protein, MBF-I, was purified employing as an affinity reagent a trout MRE that is shown to be functional in mouse cells but which lacks the G+C-rich and SP1-like sequences found in many mammalian MT gene promoters. Using point-mutated MREs, we showed that there is a strong correlation between DNA binding in vitro and MT gene regulation in vivo, suggesting a direct role of MBF-I in MT gene transcription. We also showed that MBF-I can induce MT gene transcription in vitro in a mouse extract and that this stimulation requires zinc.

Characterization of the Novel Broad-Spectrum Kinase Inhibitor CTx-0294885 As an Affinity Reagent for Mass Spectrometry-Based Kinome Profiling

2013 ◽  
Vol 12 (7) ◽  
pp. 3104-3116 ◽  
Author(s):  
Luxi Zhang ◽  
Ian P. Holmes ◽  
Falko Hochgräfe ◽  
Scott R. Walker ◽  
Naveid A. Ali ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Broad Spectrum ◽  
Kinase Inhibitor ◽  
The Novel ◽  
Affinity Reagent

scholarly journals Correlation of loss of activity of human aldehyde dehydrogenase with reaction of bromoacetophenone with glutamic acid-268 and cysteine-302 residues. Partial-sites reactivity of aldehyde dehydrogenase

1990 ◽  
Vol 266 (1) ◽  
pp. 179-187 ◽  
Author(s):  
D P Abriola ◽  
A D MacKerell ◽  
R Pietruszko
Keyword(s):  
Enzyme Activity ◽  
Glutamic Acid ◽  
Aldehyde Dehydrogenase ◽  
Scintillation Counting ◽  
Radioactive Label ◽  
Affinity Reagent ◽  
Activity Loss

Bromoacetophenone (2-bromo-1-phenylethanone) has been characterized as an affinity reagent for human aldehyde dehydrogenase (EC 1.2.1.3) [MacKerell, MacWright & Pietruszko (1986) Biochemistry 25, 5182-5189], and has been shown to react specifically with the Glu-268 residue [Abriola, Fields, Stein, MacKerell & Pietruszko (1987) Biochemistry 26, 5679-5684] with an apparent inactivation stoichiometry of two molecules of bromoacetophenone per molecule of enzyme. The specificity of bromoacetophenone for reaction with Glu-268, however, is not absolute, owing to the extreme reactivity of this reagent. When bromo[14C]acetophenone was used to label the human cytoplasmic E1 isoenzyme radioactively and tryptic fragmentation was carried out, peptides besides that containing Glu-268 were found to have reacted with reagent. These peptides were purified by h.p.l.c. and analysed by sequencing and scintillation counting to quantify radioactive label in the material from each cycle of sequencing. Reaction of bromoacetophenone with the aldehyde dehydrogenase molecule during enzyme activity loss occurs with two residues, Glu-268 and Cys-302. The activity loss, however, appears to be proportional to incorporation of label at Glu-268. The large part of incorporation of label at Cys-302 occurs after the activity loss is essentially complete. With both Glu-268 and Cys-302, however, the incorporation of label stops after one molecule of bromoacetophenone has reacted with each residue. Reaction with other residues continues after activity loss is complete.

Synthesis of P-5 tethered inositol-1,2,6-trisphosphate, an affinity reagent for α-trinositol receptors

1994 ◽  
Vol 35 (41) ◽  
pp. 7521-7524 ◽  
Author(s):  
Anu Chaudhary ◽  
György Dormán ◽  
Glenn D. Prestwich
Keyword(s):  
Affinity Reagent
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