In this study, we attempted to synthesize visible light active nano-sized photocatalysts using metal oxides such as zinc oxide, zirconium oxide, tungsten oxide, and strontium titanium oxide with (MoCl5)2 as a dopant by the simple ball-milling method. Fourier-transform infrared spectroscopy data confirmed the presence of M-O-Mo linkage (M = Zn, Zr, W, and SrTi) in all the molybdenum-doped metal oxides (MoMOs), but only MoZnO inhibited the growth of the bloom-forming Microcystis aeruginosa under visible light in a concentration-dependent manner up to 10 mg/L. Further, structural characterization of MoZnO using FESEM and XRD exhibited the formation of typical hexagonal wurtzite nanocrystals of approximately 4 nm. Hydroxyl radical (·OH), reactive oxygen species (ROS), and lipid peroxidation assays revealed ·OH generated by MoZnO under the visible light seemed to cause peroxidation of the lipid membrane of M. aeruginosa, which led to an upsurge of intracellular ROS and consequently introduced the agglomeration of cyanobacteria. These results demonstrated that nano-sized MoZnO photocatalyst can be easily synthesized in a cost-effective ball-mill method and utilized for biological applications such as the reduction of harmful algal blooms. Further, our study implies that a simple ball-milling method can provide an easy, green, and scalable route for the synthesis of visible light active doped metal oxides.
A liquid metal reaction environment for the room-temperature synthesis of atomically thin metal oxides
