Involvement of the extracellular matrix proteins periostin and tenascin C in nasal polyp remodeling by regulating the expression of MMPs

The local growth of tumors and their ability to metastasize are crucially dependent on their interactions with the surrounding extracellular matrix. Tenascin-C (TNC) is an extracellular matrix protein which is highly expressed during development, tissue repair and cancer. Despite the high levels of TNC in the stroma of primary and metastatic tumors, the function of TNC is not known. In the present study we have crossed TNC-null mice with a mouse strain where both female and male mice spontaneously develop mammary tumors followed by metastatic disease in the lungs. We report that the absence of TNC had no effect on the temporal occurrence of mammary tumors and their metastatic dissemination in lungs. Furthermore, the number and size of tumors, the number and size of metastatic foci in the lungs, the proliferation rate and apoptosis of tumor cells and tumor angiogenesis were not altered in the absence of TNC. Histological examination revealed that the tumor organisation, however, was modulated by TNC. In the presence of TNC both primary as well as metastatic tumors were organised in large tumor cell nests surrounded by thick layers of extracellular matrix proteins. In the absence of TNC these tumor cell nests were smaller but still separated from each other by extracellular matrix proteins. In addition, the TNC-null stromal compartment contained significantly more monocytes/macrophages than tumor stroma from TNC wild-type mice. Using in vitro coculture experiments we show that TNC-null tumor cells were still able to activate the TNC gene in fibroblasts which express low basal levels of TNC. Altogether these data indicate that TNC has a very limited role during the spontaneous development and growth of mamary tumors and their metastasis to the lungs.

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Alternative Splicing in the Aggrecan G3 Domain Influences Binding Interactions with Tenascin-C and Other Extracellular Matrix Proteins

Journal of Biological Chemistry ◽

10.1074/jbc.m400242200 ◽

2004 ◽

Vol 279 (13) ◽

pp. 12511-12518 ◽

Cited By ~ 75

Author(s):

Joanna M. Day ◽

Anders I. Olin ◽

Alan D. Murdoch ◽

Ann Canfield ◽

Takako Sasaki ◽

...

Keyword(s):

Extracellular Matrix ◽

Alternative Splicing ◽

Extracellular Matrix Proteins ◽

Matrix Proteins ◽

Binding Interactions ◽

Tenascin C

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Lung fibrotic tenascin-C upregulation is associated with other extracellular matrix proteins and induced by TGFβ1

BMC Pulmonary Medicine ◽

10.1186/1471-2466-14-120 ◽

2014 ◽

Vol 14 (1) ◽

Cited By ~ 33

Author(s):

Susanna Estany ◽

Vanesa Vicens-Zygmunt ◽

Roger Llatjós ◽

Ana Montes ◽

Rosa Penín ◽

...

Keyword(s):

Extracellular Matrix ◽

Extracellular Matrix Proteins ◽

Matrix Proteins ◽

Tenascin C

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Increased Obesity-Associated Circulating Levels of the Extracellular Matrix Proteins Osteopontin, Chitinase-3 Like-1 and Tenascin C Are Associated with Colon Cancer

PLoS ONE ◽

10.1371/journal.pone.0162189 ◽

2016 ◽

Vol 11 (9) ◽

pp. e0162189 ◽

Cited By ~ 13

Author(s):

Victoria Catalán ◽

Javier Gómez-Ambrosi ◽

Amaia Rodríguez ◽

Beatriz Ramírez ◽

Maitane Izaguirre ◽

...

Keyword(s):

Colon Cancer ◽

Extracellular Matrix ◽

Extracellular Matrix Proteins ◽

Matrix Proteins ◽

Tenascin C ◽

Circulating Levels

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Dynamics of extracellular matrix proteins in cerebrospinal fluid and serum and their relation to clinical outcome in human traumatic brain injury

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm-2019-0034 ◽

2019 ◽

Vol 57 (10) ◽

pp. 1565-1573 ◽

Cited By ~ 1

Author(s):

Karolina Minta ◽

Nicholas C. Cullen ◽

Faiez Al Nimer ◽

Eric P. Thelin ◽

Fredrik Piehl ◽

...

Keyword(s):

Traumatic Brain Injury ◽

Extracellular Matrix ◽

Cerebrospinal Fluid ◽

Brain Injury ◽

Clinical Outcome ◽

Extracellular Matrix Proteins ◽

Normal Pressure ◽

Matrix Proteins ◽

Healthy Controls ◽

Tenascin C

Abstract Background Brevican, neurocan, tenascin-C and tenascin-R are extracellular matrix proteins present in brain that show increased expression in experimental animal models of brain injury. However, little is known about the dynamics of these proteins in human body fluids, such as cerebrospinal fluid (CSF) and serum, after traumatic brain injury (TBI). The aims of this study were to investigate if matrix proteins in CSF and serum are associated with functional outcome following traumatic brain injury, if their concentrations change over time and to compare their levels between brain injured patients to controls. Methods In total, 42 traumatic brain injury patients, nine healthy controls and a contrast group consisting of 38 idiopathic normal pressure hydrocephalus patients were included. Enzyme-linked immunosorbent assays (ELISAs) were used to measure the concentrations of proteins. Results Increased concentrations of brevican, tenascin-C and tenascin-R in CSF correlated with unfavourable outcome, with stronger outcome prediction ability compared to other biomarkers of brain tissue injury. CSF brevican, tenascin-R and serum neurocan gradually decreased with time (p = 0.04, p = 0.008, p = 0.005, respectively), while serum tenascin-C (p = 0.01) increased. CSF concentrations of brevican, neurocan and tenascin-R (only in time point 3) after TBI were lower than in the idiopathic normal pressure hydrocephalus group (p < 0.0001, p < 0.0001, and p = 0.0008, respectively). In serum, tenascin-C concentration was higher and neurocan lower compared to healthy controls (p = 0.02 and p = 0.0009). Conclusions These findings indicate that levels of extracellular matrix proteins are associated with clinical outcome following TBI and may act as markers for different pathophysiology than currently used protein biomarkers.

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