Salt and Water Transport by Gallbladder Epithelium

2011 ◽  
Author(s):  
Luis Reuss
Keyword(s):  
Water Transport ◽  
Gallbladder Epithelium

scholarly journals Very high aquaporin-1 facilitated water permeability in mouse gallbladder

2009 ◽  
Vol 296 (4) ◽  
pp. G816-G822 ◽  
Author(s):  
Lihua Li ◽  
Hua Zhang ◽  
Tonghui Ma ◽  
A. S. Verkman
Keyword(s):  
Bile Salt ◽  
Water Transport ◽  
Water Permeability ◽  
Salt Concentration ◽  
Knockout Mice ◽  
Osmotic Gradient ◽  
Gallbladder Epithelium ◽  
Wild Type ◽  
Bile Salt Concentration ◽  
Very High

Water transport across gallbladder epithelium is driven by osmotic gradients generated from active salt absorption and secretion. Aquaporin (AQP) water channels have been proposed to facilitate transepithelial water transport in gallbladder and to modulate bile composition. We found strong AQP1 immunofluorescence at the apical membrane of mouse gallbladder epithelium. Transepithelial osmotic water permeability (Pf) was measured in freshly isolated gallbladder sacs from the kinetics of luminal calcein self-quenching in response to an osmotic gradient. Pf was very high (0.12 cm/s) in gallbladders from wild-type mice, cAMP independent, and independent of osmotic gradient size and direction. Although gallbladders from AQP1 knockout mice had similar size and morphology to those from wild-type mice, their Pf was reduced by ∼10-fold. Apical plasma membrane water permeability was greatly reduced in AQP1-deficient gallbladders, as measured by cytoplasmic calcein quenching in perfluorocarbon-filled, inverted gallbladder sacs. However, neither bile osmolality nor bile salt concentration differed in gallbladders from wild-type vs. AQP1 knockout mice. Our data indicate constitutively high water permeability in mouse gallbladder epithelium involving transcellular water transport through AQP1. The similar bile salt concentration in gallbladders from AQP1 knockout mice argues against a physiologically important role for AQP1 in mouse gallbladder.

Localization of Sodium in Normal and Inhibited Transporting Epithelia

1971 ◽  
Vol 29 ◽  
pp. 392-393
Author(s):  
G. I. Kaye ◽  
J. D. Cole
Keyword(s):  
Plasma Membrane ◽  
Similar Pattern ◽  
Fixation Method ◽  
Colonic Epithelium ◽  
Gallbladder Epithelium ◽  
Rabbit Colon ◽  
Rabbit Gallbladder

For a number of years we have used an adaptation of Komnick's KSb(OH)6-OsO4 fixation method for the localization of sodium in tissues in order to study transporting epithelia under a number of different conditions. We have shown that in actively transporting rabbit gallbladder epithelium, large quantities of NaSb(OH)6 precipitate are found in the distended intercellular compartment, while localization of precipitate is confined to the inner side of the lateral plasma membrane in inactive gallbladder epithelium. A similar pattern of distribution of precipitate has been demonstrated in human and rabbit colon in active and inactive states and in the inactive colonic epithelium of hibernating frogs.

Immunohistochemical detection of 8-hydroxy-2′-deoxyguanosine in gallbladder epithelium of patients with pancreaticobiliary maljunction

2001 ◽  
Vol 120 (5) ◽  
pp. A575-A575
Author(s):  
K OTANI ◽  
S SHIMIZU ◽  
K CHIJIIWA ◽  
K YAMAGUCHI ◽  
H NOSHIRO ◽  
...  
Keyword(s):  
Pancreaticobiliary Maljunction ◽  
Immunohistochemical Detection ◽  
Gallbladder Epithelium

Pretein kinase C isoforms α, βII and δ are expressed in gallbladder epithelium during gallstone formation

2001 ◽  
Vol 120 (5) ◽  
pp. A386-A386 ◽  
Author(s):  
S NARINS ◽  
E PARK ◽  
X SU ◽  
P SMITH ◽  
M ABEDIN
Keyword(s):  
Gallstone Formation ◽  
Gallbladder Epithelium ◽  
Kinase C

Calcium homeostasis in gallbladder epithelium is altered during earty galistone formation

2001 ◽  
Vol 120 (5) ◽  
pp. A386-A386
Author(s):  
S NARINS ◽  
J DOUGHERTY ◽  
E PARK ◽  
R NICHOLS ◽  
P SMITH ◽  
...  
Keyword(s):  
Calcium Homeostasis ◽  
Gallbladder Epithelium

PRESSURE DRIVEN WATER TRANSPORT THROUGH GRAPHENE NANOCHANNEL USING MOLECULAR DYNAMICS SIMULATIONS

2018 ◽  
Author(s):  
Subhendu Rana ◽  
Kishore Kumar Kammara ◽  
Sai Abhishek Peddakotla ◽  
Rakesh Kumar
Keyword(s):  
Molecular Dynamics ◽  
Molecular Dynamics Simulations ◽  
Water Transport ◽  
Dynamics Simulations ◽  
Pressure Driven
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