scholarly journals Base Editing in Human Cells to Produce Single‐Nucleotide‐Variant Clonal Cell Lines

2020 ◽  
Vol 133 (1) ◽  
Author(s):  
Carlos A. Vasquez ◽  
Quinn T. Cowan ◽  
Alexis C. Komor
Keyword(s):  
Cell Lines ◽  
Human Cells ◽  
Single Nucleotide Variant ◽  
Single Nucleotide ◽  
Base Editing ◽  
Clonal Cell ◽  
Clonal Cell Lines

ESTABLISHMENT AND CHARACTERIZATION OF CLONAL CELL LINES FROM THE VAGINA OF p53-DEFICIENT YOUNG MICE

2002 ◽  
Vol 38 (10) ◽  
pp. 547 ◽  
Author(s):  
KAYO TANAHASHI ◽  
SHINOBU SHIBAHARA ◽  
MINAKO OGAWA ◽  
MAKOTO HANAZONO ◽  
SHINICHI AIZAWA ◽  
...  
Keyword(s):  
Cell Lines ◽  
Clonal Cell ◽  
Clonal Cell Lines ◽  
Young Mice

Implantation of c-mycERTAMImmortalized Human Mesencephalic-Derived Clonal Cell Lines Ameliorates Behavior Dysfunction in a Rat Model of Parkinson’s Disease

2009 ◽  
Vol 18 (2) ◽  
pp. 307-320 ◽  
Author(s):  
Erik A. Miljan ◽  
Susan J. Hines ◽  
Priyadarshini Pande ◽  
Randolph L. Corteling ◽  
Caroline Hicks ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Cell Lines ◽  
Rat Model ◽  
Clonal Cell ◽  
Clonal Cell Lines

Differentiation and Interaction of Clonal Cell Lines of Nerve and Muscle

1973 ◽  
pp. 55-86 ◽  
Author(s):  
David Schubert ◽  
A. John Harris ◽  
Stephen Heinemann ◽  
Y. Kidokoro ◽  
James Patrick ◽  
...  
Keyword(s):  
Cell Lines ◽  
Clonal Cell ◽  
Clonal Cell Lines

Different Osteochondral Potential of Clonal Cell Lines Derived from Adult Human Trabecular Bone

2002 ◽  
Vol 961 (1) ◽  
pp. 73-77 ◽  
Author(s):  
ANNA M. OSYCZKA ◽  
ULRICH NÖTH ◽  
KEITH G. DANIELSON ◽  
ROCKY S. TUAN
Keyword(s):  
Trabecular Bone ◽  
Cell Lines ◽  
Clonal Cell ◽  
Adult Human ◽  
Human Trabecular Bone ◽  
Clonal Cell Lines

Clonal cell lines from the rat central nervous system

Nature ◽  
1974 ◽  
Vol 249 (5454) ◽  
pp. 224-227 ◽  
Author(s):  
D. Schubert ◽  
S. Heinemann ◽  
W. Carlisle ◽  
H. Tarikas ◽  
B. Kimes ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Cell Lines ◽  
Clonal Cell ◽  
Clonal Cell Lines

scholarly journals Stepwise activation of the mouse acetylcholine receptor delta- and gamma-subunit genes in clonal cell lines.

1988 ◽  
Vol 8 (12) ◽  
pp. 5257-5267 ◽  
Author(s):  
C M Crowder ◽  
J P Merlie
Keyword(s):  
Acetylcholine Receptor ◽  
Cell Lines ◽  
Subunit Gene ◽  
Rnase Protection ◽  
Gamma Subunit ◽  
Clonal Cell ◽  
Hypersensitive Sites ◽  
Clonal Cell Lines ◽  
Gamma Locus ◽  
Delta Subunit

We used the DNase I-hypersensitive sites around the mouse acetylcholine receptor delta-subunit gene as a guide toward the cloning and sequencing of delta and gamma transcriptional regulatory regions and as a means to assess chromatin structural activation of the delta- and gamma-subunit genes during myogenesis. Genomic cloning of hypersensitive sites downstream of the delta-subunit gene revealed the presence of the gamma-subunit gene approximately 5 kilobases away; the hypersensitive sites mapped to the 5' end of the gamma-subunit gene. Sequence comparison of restriction fragments containing hypersensitive sites in analogous locations at the 5' ends of the delta- and gamma-subunit genes uncovered little overall homology between the two genomic fragments; however, an 11- of 13-base-pair match between the two sequences was found. Homologs to this sequence were also found to be present in the upstream regions of the chicken alpha- and mouse beta-subunit genes. By RNase protection and primer extension analyses, the delta-subunit gene transcription start site was mapped to 56 base pairs upstream of the initiator ATG codon. Clonal cell lines with various potentials to differentiate to the skeletal muscle phenotype were examined for their hypersensitive site pattern within the delta-gamma locus. Only remote hypersensitive sites flanking the locus appear in pluripotential mesodermal cells. A cell line of determined but inducible myoblasts expressed only one more intergenic site, while in permissively differentiating myoblasts hypersensitive sites were already present at the 5' ends of the delta and gamma genes prior to differentiation. Terminal differentiation resulted in an identical pattern of hypersensitive sites in all muscle cell lines examined so far, with an intergenic site near the gamma-subunit gene being the only site specific to the differentiated muscle phenotype.

Proteins secreted by clonal cell lines

1976 ◽  
Vol 102 (2) ◽  
pp. 329-340 ◽  
Author(s):  
D. Schubert
Keyword(s):  
Cell Lines ◽  
Clonal Cell ◽  
Clonal Cell Lines
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