scholarly journals Epicardial and endothelial cell activation concurs with extracellular matrix remodeling in atrial fibrillation

2021 ◽  
Vol 11 (11) ◽  
Author(s):  
Nicoline W. E. van den Berg ◽  
Makiri Kawasaki ◽  
Benedetta Fabrizi ◽  
Fransisca A. Nariswari ◽  
Arianne C. Verduijn ◽  
...  
Keyword(s):  
Atrial Fibrillation ◽  
Extracellular Matrix ◽  
Endothelial Cell ◽  
Cell Activation ◽  
Extracellular Matrix Remodeling ◽  
Matrix Remodeling ◽  
Endothelial Cell Activation

Digoxin use is associated with increased platelet and endothelial cell activation in patients with nonvalvular atrial fibrillation

Heart Rhythm ◽  
2005 ◽  
Vol 2 (5) ◽  
pp. 525-529 ◽  
Author(s):  
Julio A. Chirinos ◽  
Aurelio Castrellon ◽  
Juan Pablo Zambrano ◽  
Joaquin J. Jimenez ◽  
Wenche Jy ◽  
...  
Keyword(s):  
Atrial Fibrillation ◽  
Endothelial Cell ◽  
Cell Activation ◽  
Endothelial Cell Activation ◽  
Nonvalvular Atrial Fibrillation

scholarly journals Electrophysiological and Structural Remodeling of the Atria in a Mouse Model of Troponin-I Mutation Linked Hypertrophic Cardiomyopathy: Implications for Atrial Fibrillation

2021 ◽  
Vol 22 (13) ◽  
pp. 6941
Author(s):  
Wei-Wen Lim ◽  
Melissa Neo ◽  
Shivshankar Thanigaimani ◽  
Pawel Kuklik ◽  
Anand N. Ganesan ◽  
...  
Keyword(s):  
Atrial Fibrillation ◽  
Extracellular Matrix ◽  
Hypertrophic Cardiomyopathy ◽  
Troponin I ◽  
Interstitial Fibrosis ◽  
Electrophysiological Study ◽  
Electrode Array ◽  
Extracellular Matrix Remodeling ◽  
Matrix Remodeling ◽  
Structural Remodeling

Hypertrophic cardiomyopathy (HCM) is an inherited cardiac disorder affecting one in 500 of the general population. Atrial fibrillation (AF) is the most common arrhythmia in patients with HCM. We sought to characterize the atrial electrophysiological and structural substrate in young and aging Gly203Ser cardiac troponin-I transgenic (HCM) mice. At 30 weeks and 50 weeks of age (n = 6 per strain each group), the left atrium was excised and placed on a multi-electrode array (MEA) for electrophysiological study; subsequent histological analyses and plasma samples were analyzed for biomarkers of extracellular matrix remodeling and cell adhesion and inflammation. Wild-type mice of matched ages were included as controls. Young HCM mice demonstrated significantly shortened atrial action potential duration (APD), increased conduction heterogeneity index (CHI), increased myocyte size, and increased interstitial fibrosis without changes in effective refractory periods (ERP), conduction velocity (CV), inflammatory infiltrates, or circulating markers of extracellular matrix remodeling and inflammation. Aging HCM mice demonstrated aggravated changes in atria electrophysiology and structural remodeling as well as increased circulating matrix metalloproteinases (MMP)-2, MMP-3, and VCAM-1 levels. This model of HCM demonstrates an underlying atrial substrate that progresses with age and may in part be responsible for the greater propensity for AF in HCM.

scholarly journals Atrial Extracellular Matrix Remodeling and the Maintenance of Atrial Fibrillation

Circulation ◽  
2004 ◽  
Vol 109 (3) ◽  
pp. 363-368 ◽  
Author(s):  
Jun Xu ◽  
Guanggen Cui ◽  
Fardad Esmailian ◽  
Mark Plunkett ◽  
Daniel Marelli ◽  
...  
Keyword(s):  
Atrial Fibrillation ◽  
Extracellular Matrix ◽  
Extracellular Matrix Remodeling ◽  
Matrix Remodeling

scholarly journals Endothelial Cell Activation by Interleukin-1 and Extracellular Matrix Laminin-10 Occurs via the Yap Signalling Pathway

2021 ◽  
Author(s):  
Hannah Thurgur ◽  
Jeffrey Penny ◽  
Emmanuel Pinteaux
Keyword(s):  
Extracellular Matrix ◽  
Endothelial Cell ◽  
Cell Activation ◽  
Interleukin 1 ◽  
Tube Formation ◽  
Signalling Pathway ◽  
Endothelial Cell Activation ◽  
First Time

Abstract Background: The extracellular matrix (ECM) plays an important role for normal brain functions and homeostasis, and contributes to the inflammatory response and mechanisms of brain repair after acute brain injury. We have previously reported that the ECM laminin-10 (LM-10) is a key regulator of blood-brain barrier (BBB) integrity, and is involved in BBB repair after hypoxic injury and interleukin-1 (IL-1)-induced inflammation in vitro. To further investigate the role of LM-10 in BBB inflammation and repair, we investigated for the first time the signalling mechanisms regulated by LM-10 in brain endothelial cells in response to IL-1β-induced inflammation in vitro. Methods: Human brain endothelial cell line hCMEC/D3 cultured on Matrigel- or LM-10-coated tissue culture plates were left untreated or were treated with human recombinant IL-1β at various concentrations and/or for various periods of time. In vitro hallmarks of angiogenesis were assessed using a scratch injury model and tube formation assay. Expression of cell adhesion molecules ICAM-1 and VCAM-1, as well as IL-8 was measured using ELISA. Activation of signalling pathways ERK1/2, p38, NF-κB and YAP was assessed by quantitative ELISA or Western blot. Activation of genes downstream of YAP signalling was assessed by quantitative polymerase chain reaction.Results: LM-10 promoted endothelial proliferation and subsequent repair of an endothelial monolayer after scratch injury, induced tube formation, and upregulated IL-1β-induced ICAM-1 and VCAM-1 expression in vitro. Classical IL-1β-induced signalling pathway ERK1/2 and p38 were not modulated by LM-10, whilst LM-10 upregulated IL-1β-induced NF-κB activation. Importantly, we demonstrate for the first time a role of the YAP signalling pathway in endothelial cell activation, in that LM-10 significantly downregulates p-YAP (S397) activation without affecting phosphorylation of YAP (S127), leading to differential expression of YAP target genes, ctgf and serpine-1 involved in endothelial cell activation. Conclusion: Our study provides for the first time evidence that the YAP signalling pathway is an important regulator of endothelial cell activation, and could be a new therapeutic target for the treatment of cerebrovascular inflammatory diseases. 

scholarly journals Extracellular Matrix Remodeling precedes Atrial Fibrillation. Results of the PREDICT-AF trial.

Heart Rhythm ◽  
2021 ◽  
Author(s):  
Nicoline W.E. van den Berg ◽  
Jolien Neefs ◽  
Makiri Kawasaki ◽  
Fransisca A. Nariswari ◽  
Robin Wesselink ◽  
...  
Keyword(s):  
Atrial Fibrillation ◽  
Extracellular Matrix ◽  
Extracellular Matrix Remodeling ◽  
Matrix Remodeling
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