Cholesterol-lowering effect of an acyl-coenzyme A: Cholesterol acyltransferase inhibitor, FR145237, in hypercholesterolemic rabbits fed a high-cholesterol diet and a cholesterol-free casein diet

Objective: Moringa oleifera Lam. (Moringaceae), a small rapid growing, evergreen, deciduous tree is an important medicinal plant. Leaves and fruits of this plant are used for various ailments, as a nutritional supplement and also as vegetables. The current study involves in the determination of best combination of the cholesterol-lowering potential of a blend of methanol extracts of M. oleifera leaf and fruits, developed based on in vitro FIC index studies and evaluate the combination of this extracts in hypercholesterolemic animal models.Methods: Leaf and fruit methanol extracts and their combinations were tested in in vitro lipase inhibition assay to determine the best combination using fractional inhibitory concentration (FIC) index. Hypercholesterolemia was induced with Triton WR-1339 (a non-ionic detergent) and with high cholesterol diet for acute and chronic model respectively and the cholesterol-lowering effect of 1:1 blend of M. oleifera leaf and fruits methanol extracts was evaluated.Results: The FIC index values indicated that M. oleifera leaf and fruit extracts blended in 1:1 proportion was the best combination in in vitro lipase inhibition assay. This blend, when evaluated in vivo, showed a significant decrease in serum total cholesterol level from 24 h through 48 h in triton model. In high cholesterol diet model, the extract blend showed a significant reduction in serum triglycerides levels at 3 and 6 w of treatment.Conclusion: The results indicate that the blend of M. oleifera at the tested dose could be lowering cholesterol and triglyceride levels by inhibiting the absorption of cholesterol and can be developed as a standardized blend for dietary supplement market.

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Antiatherosclerotic Effect ofCanarium odontophyllumMiq. Fruit Parts in Rabbits Fed High Cholesterol Diet

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2012/838604 ◽

2012 ◽

Vol 2012 ◽

pp. 1-10 ◽

Cited By ~ 9

Author(s):

Faridah Hanim Shakirin ◽

Azrina Azlan ◽

Amin Ismail ◽

Zulkhairi Amom ◽

Lau Cheng Yuon

Keyword(s):

Normal Diet ◽

High Cholesterol Diet ◽

Cholesterol Lowering ◽

Antioxidants Activity ◽

Lowering Effect ◽

Hypercholesterolemic Diet ◽

Antiatherosclerotic Effect ◽

Antioxidant Agent ◽

Potential Factors ◽

Cholesterol Lowering Effect

The effect ofC. odontophyllum(CO) fruit parts was investigated in hypercholesterolemic rabbits. Forty-nine rabbits, which were randomly divided into seven groups of seven animals (n=7), received a diet containing different parts of CO fruit parts for 8 weeks. The groups were as follows: (1) normal diet: NC group and (2) hypercholesterolemic diet: PC, HS (10 mg/kg/day simvastatin), HPO (20 g kg−1oil extracted from the pulp of CO), HKO (20 g kg−1oil extracted from the kernel of CO), HF (50 g kg−1fullfat pulp of CO), and HD (50 g kg−1defatted pulp of CO). Among these groups, rabbits receiving defatted pulp of CO showed the greatest cholesterol lowering effect as it had reduced plasma LDL-C, TC, and thiobarbiturate reactive substance (TBARS) levels as well as atherosclerotic plaques. The presence of high dietary fiber and antioxidants activity are potential factors contributing to the cholesterol lowering effect. Consequently, these results indicate the potential use of CO defatted pulp as a cholesterol lowering and antioxidant agent.

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