Chlorogenic Acid Exhibits Cholesterol Lowering and Fatty Liver Attenuating Properties by Up-regulating the Gene Expression of PPAR-α in Hypercholesterolemic Rats Induced with a High-Cholesterol Diet

2012 ◽  
Vol 27 (4) ◽  
pp. 545-551 ◽  
Author(s):  
Chun-Wai Wan ◽  
Candy Ngai-Yan Wong ◽  
Wing-Kwan Pin ◽  
Marcus Ho-Yin Wong ◽  
Ching-Yee Kwok ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fatty Liver ◽  
Chlorogenic Acid ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Cholesterol Lowering

Abstract 130: Hypercholesterolemia Suppresses Carotid Artery Endothelial NOS3 Expression via Epigenetic Mechanisms

2015 ◽  
Vol 35 (suppl_1) ◽  
Author(s):  
Alex Sotolongo ◽  
Yi-Zhou Jiang ◽  
John Karanian ◽  
William Pritchard ◽  
Peter Davies
Keyword(s):  
Gene Expression ◽  
Dna Methylation ◽  
Endothelial Cells ◽  
Dna Methyltransferase ◽  
Control Group ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
High Fat

Objective: One of the first clinically detectable changes in the vasculature during atherogenesis is the accumulation of cholesterol within the vessel wall. Hypercholesterolemia is characterized by dysfunctional endothelial-dependent vessel relaxation and impaired NOS3 function. Since DNA methylation at gene promoter regions strongly suppresses gene expression, we postulated that high-fat/high-cholesterol diet suppresses endothelial NOS3 through promoter DNA methylation. Methods: Domestic male pigs were fed control diet (CD) or isocaloric high fat and high cholesterol diet (HC; 12% fat and 1.5% cholesterol) for 2, 4, 8 or 12 weeks prior to tissue collection. Furthermore, to determine the effects of risk factor withdrawal, an additional group of swine received HC for 12 weeks and then CD for 8 weeks; a control group received HC continuously for 20 weeks. Endothelial cells were harvested from common carotid aorta. In parallel in vitro studies, cultured human aortic endothelial cells (HAEC) were treated with human LDL, GW3956 (LXR agonist) and RG108 (DNA methyltransferase [DNMT] inhibitor). In cells from both sources, DNA methylation at the NOS3 promoter was measured using methylation specific pyro sequencing, and endothelial gene expression was measured using RT PCR. Results: HC diet increased plasma cholesterol level from 75 mg/dl on CD to a plateau of about 540 mg/dl within 2 weeks. Endothelial NOS3 expression was significantly reduced (71±9 % of CD) after 4 weeks of HC, a level sustained at subsequent time points. Withdrawal of HC for 8 weeks did not recover NOS3 expression. After 12-week HC, the NOS3 promoter was hypermethylated. Withdrawal of HC did not reverse NOS3 promoter methylation. In vitro treatment of HAEC with human LDL (200 mg/dl total cholesterol) or GW3956 (5μM) suppressed NOS3 mRNA to 50% and 30% respectively, suggesting that LXR/RXR is involved in suppression of NOS3. Nitric oxide production was consistently suppressed by GW3959. Both could be reversed through inhibition of DNMTs by RG108. Conclusions: DNA methylation and LXR/RXR pathway can mediate the HC-suppression of endothelial NOS3. The study identifies novel pharmaceutical targets in treating endothelial dysfunction. Crosstalk between these pathways is under investigation.

Up-Regulation of Hepatic LDL Receptor Gene Expression by Monatepil, a Novel Calcium Antagonist, in High Cholesterol Diet-Fed Japanese Monkeys

1994 ◽  
Vol 7 (11) ◽  
pp. 1026-1030 ◽  
Author(s):  
Mitsue Notake ◽  
Yasushi Kondo ◽  
Hideki Nomura ◽  
Katsuji Nakano ◽  
Kanoo Hosoki ◽  
...  
Keyword(s):  
Gene Expression ◽  
Calcium Antagonist ◽  
Receptor Gene ◽  
Ldl Receptor ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Japanese Monkeys ◽  
Receptor Gene Expression

scholarly journals CHOLESTEROL LOWERING POTENTIALS OF A BLEND OF STANDARDIZED METHANOL EXTRACTS OF MORINGA OLEIFERA LEAVES AND FRUITS IN ALBINO WISTAR RATS

2016 ◽  
Vol 8 (11) ◽  
pp. 262
Author(s):  
Gururaja G. M. ◽  
Deepak Mundkinajeddu ◽  
Senthil Kumar A. ◽  
Joshua Allan J. ◽  
Shekhar M. Dethe ◽  
...  
Keyword(s):  
Moringa Oleifera ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
Inhibition Assay ◽  
High Cholesterol ◽  
Cholesterol Lowering ◽  
Methanol Extracts ◽  
Ionic Detergent ◽  
Lipase Inhibition

Objective: Moringa oleifera Lam. (Moringaceae), a small rapid growing, evergreen, deciduous tree is an important medicinal plant. Leaves and fruits of this plant are used for various ailments, as a nutritional supplement and also as vegetables. The current study involves in the determination of best combination of the cholesterol-lowering potential of a blend of methanol extracts of M. oleifera leaf and fruits, developed based on in vitro FIC index studies and evaluate the combination of this extracts in hypercholesterolemic animal models.Methods: Leaf and fruit methanol extracts and their combinations were tested in in vitro lipase inhibition assay to determine the best combination using fractional inhibitory concentration (FIC) index. Hypercholesterolemia was induced with Triton WR-1339 (a non-ionic detergent) and with high cholesterol diet for acute and chronic model respectively and the cholesterol-lowering effect of 1:1 blend of M. oleifera leaf and fruits methanol extracts was evaluated.Results: The FIC index values indicated that M. oleifera leaf and fruit extracts blended in 1:1 proportion was the best combination in in vitro lipase inhibition assay. This blend, when evaluated in vivo, showed a significant decrease in serum total cholesterol level from 24 h through 48 h in triton model. In high cholesterol diet model, the extract blend showed a significant reduction in serum triglycerides levels at 3 and 6 w of treatment.Conclusion: The results indicate that the blend of M. oleifera at the tested dose could be lowering cholesterol and triglyceride levels by inhibiting the absorption of cholesterol and can be developed as a standardized blend for dietary supplement market.

Effect of T0901317 on Hepatic Proinflammatory Gene Expression in ApoE−/− Mice Fed a High-fat/high-cholesterol Diet

Inflammation ◽  
2007 ◽  
Vol 30 (3-4) ◽  
pp. 105-117 ◽  
Author(s):  
Xiaoyan Dai ◽  
Xiang Ou ◽  
Xinrui Hao ◽  
Dongli Cao ◽  
Yaling Tang ◽  
...  
Keyword(s):  
Gene Expression ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
High Fat ◽  
Proinflammatory Gene ◽  
Proinflammatory Gene Expression

Beneficial effects of soy milk and fiber on high cholesterol diet-induced alteration of gut microbiota and inflammatory gene expression in rats

2015 ◽  
Vol 6 (2) ◽  
pp. 492-500 ◽  
Author(s):  
Seung-Min Lee ◽  
Hye Won Han ◽  
Seung Yun Yim
Keyword(s):  
Gene Expression ◽  
Gut Microbiota ◽  
High Cholesterol Diet ◽  
Soy Milk ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Inflammatory Gene Expression ◽  
Inflammatory Gene ◽  
Beneficial Effects ◽  
Induced Changes

We sought to evaluate whether a soy milk and fiber mixture could improve high cholesterol diet-induced changes in gut microbiota and inflammation.

Selection of Cholesterol-Lowering Lactic Acid Bacteria and its Effects on Rats Fed with High-Cholesterol Diet

2017 ◽  
Vol 74 (5) ◽  
pp. 623-631 ◽  
Author(s):  
Yufang Liu ◽  
Fengchun Zhao ◽  
Jiye Liu ◽  
Huimin Wang ◽  
Xiao Han ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Cholesterol Lowering ◽  
Selection Of

Cholesterol-lowering potentials of Lactobacillus strain overexpression of bile salt hydrolase on high cholesterol diet-induced hypercholesterolemic mice

2019 ◽  
Vol 10 (3) ◽  
pp. 1684-1695 ◽  
Author(s):  
Guangqiang Wang ◽  
Wenli Huang ◽  
Yongjun Xia ◽  
Zhiqiang Xiong ◽  
Lianzhong Ai
Keyword(s):  
Bile Salt ◽  
Lactobacillus Strain ◽  
Bile Salt Hydrolase ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Cholesterol Lowering

Lactobacillus strain overexpression of bile salt hydrolase can exert a cholesterol-reducing effect in vivo.

Sign in / Sign up

Export Citation Format

Share Document