25C-NBOMe and 25I-NBOMe metabolite studies in human hepatocytes, in vivo mouse and human urine with high-resolution mass spectrometry

2016 ◽  
Vol 9 (5) ◽  
pp. 680-698 ◽  
Author(s):  
Ariane Wohlfarth ◽  
Markus Roman ◽  
Mikael Andersson ◽  
Fredrik C. Kugelberg ◽  
Xingxing Diao ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Human Urine ◽  
High Resolution Mass Spectrometry ◽  
Human Hepatocytes ◽  
High Resolution Mass ◽  
Resolution Mass

scholarly journals Identification and Distribution of Novel Metabolites of Lolitrem B in Mice by High-Resolution Mass Spectrometry

Molecules ◽  
2020 ◽  
Vol 25 (2) ◽  
pp. 372 ◽  
Author(s):  
Priyanka Reddy ◽  
Aaron Elkins ◽  
Joanne Hemsworth ◽  
Kathryn Guthridge ◽  
Simone Vassiliadis ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
High Resolution Mass Spectrometry ◽  
Brain Regions ◽  
High Resolution Mass ◽  
Metabolic Products ◽  
Severe Intoxication ◽  
Lolitrem B ◽  
Resolution Mass

Lolitrem B is the most potent indole-diterpene mycotoxin produced by Epichloë festucae var. lolii (termed LpTG-1), with severe intoxication cases reported in livestock. To date, there are no in vivo metabolism studies conducted for the mycotoxin. A mouse model assay established for assessing toxicity of indole-diterpenes was used to investigate metabolic products of lolitrem B. Mice were administered lolitrem B at 0.5 and 2.0 mg/kg body weight (b.wt) intraperitoneally before body and brain tissues were collected at 6 h and 24 h post-treatment. Samples were cryoground and subjected to a biphasic or monophasic extraction. The aqueous and lipophilic phases were analysed using liquid chromatography high-resolution mass spectrometry (LC–HRMS); data analysis was performed with Compound Discoverer™ software. A total of 10 novel phase I metabolic products were identified in the lipophilic phase and their distribution in the liver, kidney and various brain regions are described. The biotransformation products of lolitrem B were found to be present in low levels in the brain. Based on structure–activity postulations, six of these may contribute towards the protracted tremors exhibited by lolitrem B-exposed animals.

In vitro and in vivo metabolism of 3-quinuclidinyl benzilate by high-resolution mass spectrometry

2020 ◽  
Vol 190 ◽  
pp. 113519
Author(s):  
Martin Uher ◽  
Martin Mžik ◽  
Jana Žďárová Karasová ◽  
David Herman ◽  
Lenka Čechová ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
High Resolution Mass Spectrometry ◽  
In Vivo Metabolism ◽  
High Resolution Mass ◽  
Resolution Mass ◽  
Quinuclidinyl Benzilate

First metabolic profile of PV8, a novel synthetic cathinone, in human hepatocytes and urine by high-resolution mass spectrometry

2016 ◽  
Vol 408 (18) ◽  
pp. 4845-4856 ◽  
Author(s):  
Madeleine J. Swortwood ◽  
Kayla N. Ellefsen ◽  
Ariane Wohlfarth ◽  
Xingxing Diao ◽  
Marta Concheiro-Guisan ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Metabolic Profile ◽  
High Resolution Mass Spectrometry ◽  
Human Hepatocytes ◽  
High Resolution Mass ◽  
Synthetic Cathinone ◽  
Resolution Mass
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