Response to letter to the editor: “Comments on Unambiguous identification and characterization of a long-term human metabolite of dehydrochloromethyltestosterone”

Abstract HSCs are defined by their ability to self-renew and maintain hematopoiesis throughout the lifespan of an organism. The optical clarity of their embryos and the ease of genetic manipulation make the zebrafish (Danio rerio) an excellent model for studying hematopoiesis. Using flow cytometry, we identified 2 populations of CD41-GFP+ cells (GFPhi and GFPlo) in the whole kidney marrow of Tg(CD41:GFP) zebrafish. Past studies in humans and mice have shown that CD41 is transiently expressed in the earliest hematopoietic progenitors and is then silenced, reappearing in the platelet/thrombocyte lineage. We have transplanted flow-sorted GFPhi and GFPlo cells into irradiated adult zebrafish and assessed long-term hematopoietic engraftment. Transplantation of GFPhi cells did not reconstitute hematopoiesis. In contrast, we observed multilineage hematopoiesis up to 68 weeks after primary and secondary transplantation of GFPlo cells. We detected the CD41-GFP transgene in all major hematopoietic lineages and CD41-GFP+ cells in histologic sections of kidneys from transplant recipients. These studies show that CD41-GFPlo cells fulfill generally accepted criteria for HSCs. The identification of fluorescent zebrafish HSCs, coupled with our ability to transplant them into irradiated adult recipients, provide a valuable new tool to track HSC homing, proliferation, and differentiation into hematopoietic cells.

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On the Identification and Characterization of Drought and Aridity in Postglacial Paleoenvironmental Records from the Northern Great Plains

Géographie physique et Quaternaire ◽

10.7202/009108ar ◽

2004 ◽

Vol 56 (2-3) ◽

pp. 229-246 ◽

Cited By ~ 8

Author(s):

Alwynne B. Beaudoin

Keyword(s):

Global Warming ◽

High Resolution ◽

Great Plains ◽

Northern Great Plains ◽

Short Term ◽

Instrumental Data ◽

Proxy Records ◽

Identification And Characterization

Abstract The Northern Great Plains region is especially sensitive to drought and is likely to be even more drought-prone under projected global warming. Drought has been invoked as an explanatory factor for changes seen in postglacial paleoenvironmental records. These proxy records may extend drought history derived from instrumental data. Moreover, in the last decade, some paleoenvironmental studies have been expressly undertaken for the examination of long-term drought history. Nevertheless, few such studies explicitly define drought. This makes it difficult to compare results or to understand what the results mean in terms of the operational drought definitions that are used in resource management. Operational drought is defined as usually short-term; longer sustained dry intervals reflect a shift to aridity. Therefore, high resolution paleoenvironmental proxies (annual or subdecadal) are best for the investigation of drought history. Such proxies include tree rings and some lake records. However, most lake-based records are sampled at lower resolution (decadal or subcentury) and are therefore providing aridity signals.

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Mass spectrometric identification and characterization of a new long-term metabolite of metandienone in human urine

Rapid Communications in Mass Spectrometry ◽

10.1002/rcm.2587 ◽

2006 ◽

Vol 20 (15) ◽

pp. 2252-2258 ◽

Cited By ~ 85

Author(s):

Wilhelm Schänzer ◽

Hans Geyer ◽

Gregor Fußhöller ◽

Natalia Halatcheva ◽

Maxie Kohler ◽

...

Keyword(s):

Human Urine ◽

Mass Spectrometric ◽

Spectrometric Identification ◽

Mass Spectrometric Identification ◽

Identification And Characterization

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Long-term regulation of aquaporins in the kidney

AJP Renal Physiology ◽

10.1152/ajprenal.1999.276.3.f331 ◽

1999 ◽

Vol 276 (3) ◽

pp. F331-F339 ◽

Cited By ~ 18

Author(s):

David Marples ◽

Jørgen Frøkiaer ◽

Søren Nielsen

Keyword(s):

Water Balance ◽

Water Permeability ◽

Clinical Management ◽

Collecting Duct ◽

Clinical Disorders ◽

Long Term Changes ◽

Identification And Characterization ◽

Aquaporin Family

The discovery of the aquaporin family of water channels has greatly improved our understanding of how water crosses epithelial cells, particularly in the kidney. The study of the mechanisms involved in the regulation of collecting duct water permeability, in particular, has advanced very rapidly since the identification and characterization of aquaporin-2 (AQP2) in 1993. One of the more surprising findings has been the dramatic long-term changes that are seen in the abundance of this protein, as well as the recognition that these changes represent a way of modulating the acute antidiuretic effects of vasopressin. Furthermore, such changes seem to be of etiological and pathological significance in a number of clinical disorders of water balance. This review focuses on the various conditions in which AQP2 expression is altered (either increased or decreased) and on what this can tell us about the signals and mechanisms controlling these changes. Ultimately, this may be of great value in the clinical management of water balance disorders. Evidence is also now beginning to emerge that there are similar changes in the expression of other renal aquaporins, which had previously been thought to provide an essentially constitutive water permeability pathway, suggesting that they too should be considered as regulatory factors in the control of body water balance.

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Identification and characterization of murine stem cells with preferential short-term or long-term bone marrow repopulating ability

European Journal of Cancer ◽

10.1016/0959-8049(95)99864-v ◽

1995 ◽

Vol 31 ◽

pp. S5

Author(s):

W.E. Fibbe ◽

J.M.J.M. Zijlmans ◽

J.W.M. Visser ◽

R. Willemze

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Short Term ◽

Marrow Repopulating Ability ◽

Identification And Characterization

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Identification and Characterization of Long Term Survival Population In Non-Small-Cell Lung Cancer Patients Treated With Immunotherapies

Value in Health ◽

10.1016/j.jval.2015.03.1118 ◽

2015 ◽

Vol 18 (3) ◽

pp. A193

Author(s):

P. Luaces ◽

L. Sanchez ◽

C. Viada ◽

P.C. Rodriguez ◽

M. Alvarez ◽

...

Keyword(s):

Lung Cancer ◽

Small Cell Lung Cancer ◽

Cancer Patients ◽

Small Cell ◽

Small Cell Lung ◽

Term Survival ◽

Lung Cancer Patients ◽

Identification And Characterization

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Ing4-deficiency enhances HSC quiescence and confers resistance to inflammatory stress

10.1101/2021.07.14.452366 ◽

2021 ◽

Author(s):

Zanshé Thompson ◽

Georgina A. Anderson ◽

Melanie Rodriguez ◽

Seth Gabriel ◽

Vera Binder ◽

...

Keyword(s):

Gene Expression ◽

Stem Cells ◽

Malignant Cells ◽

Short Term ◽

Regenerative Capacity ◽

Hematopoietic Stem ◽

Inflammatory Stress ◽

Identification And Characterization

Hematopoiesis is tightly regulated by a network of transcription factors and complexes that are required for the development and maintenance of hematopoietic stem cells (HSCs). We recently identified the tumor suppressor, Ing4, as a critical regulator of HSC homeostasis. Though the Ing4 mechanism of action remains poorly characterized, it has been shown to promote stem-like cell characteristics in malignant cells. This activity is, in part, due to Ing4 mediated regulation of several major signaling pathways, including NF-kB and c-Myc. In murine hematopoiesis, Ing4 deficiency induces G0 arrest in HSCs, while simultaneously promoting gene expression signatures associated with differentiation. This results in a poised state for Ing4-deficient HSCs. Long term HSCs are unable to overcome this block, but short-term HSCs convert the poised state into regenerative capacity during hematopoietic challenges, including irradiation and transplantation. Overall, our findings suggest that Ing4 plays a crucial role in the regulation of hematopoiesis. Our model provides key tools for further identification and characterization of pathways that control quiescence and differentiation in HSCs.

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