Expression pattern of the V5‐Ostm1 protein in bacterial artificial chromosome transgenic mice

genesis ◽  
2021 ◽  
Author(s):  
Monica Pata ◽  
Pardis Yousefi Behzadi ◽  
Jean Vacher
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Transgenic Mice ◽  
Expression Pattern ◽  
Artificial Chromosome

scholarly journals APOL1 is not expressed in proximal tubules and is not filtered

2019 ◽  
Author(s):  
Natalya A. Blessing ◽  
Zhenzhen Wu ◽  
Sethu Madhavan ◽  
Myung K. Shin ◽  
Maarten Hoek ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Bacterial Artificial Chromosome ◽  
Transgenic Mice ◽  
Proximal Tubule ◽  
Expression Pattern ◽  
Artificial Chromosome ◽  
Proximal Tubules ◽  
Mechanistic Studies

AbstractThe kidney expression pattern of APOL1 was examined using both protein and mRNAin situmethods onAPOL1bacterial artificial chromosome transgenic mice, with and without proteinuria. APOL1 was detected in podocytes and endothelial cells of the kidney, but was not expressed in tubular epithelia, nor was plasma APOL1 protein filtered and reabsorbed by the proximal tubule. APOL1 expression in podocytes and endothelia should remain the focus for mechanistic studies of APOL1-mediated pathogenesis.

The murine platelet and plasma factor V pools are biosynthetically distinct and sufficient for minimal hemostasis

Blood ◽  
2003 ◽  
Vol 102 (8) ◽  
pp. 2856-2861 ◽  
Author(s):  
Hongmin Sun ◽  
Tony L. Yang ◽  
Angela Yang ◽  
Xixi Wang ◽  
David Ginsburg
Keyword(s):  
Gene Expression ◽  
Bacterial Artificial Chromosome ◽  
Transgenic Mice ◽  
Coagulation Factor ◽  
Artificial Chromosome ◽  
Coagulation Cascade ◽  
Factor V ◽  
Wild Type ◽  
Plasma Factor ◽  
Coagulation Factor V

Abstract Coagulation factor V (FV) is a central regulator of the coagulation cascade. Circulating FV is found in plasma and within platelet α granules. The specific functions of these distinct FV pools are uncertain. We now report the generation of transgenic mice with FV gene expression restricted to either the liver or megakaryocyte/platelet lineage using bacterial artificial chromosome (BAC) constructs. Six of 6 independent albumin BAC transgenes rescue the neonatal lethal hemorrhage of FV deficiency. Rescued mice all exhibit liver-specific Fv expression at levels ranging from 6% to 46% of the endogenous Fv gene, with no detectable FV activity within the platelet pool. One of the 3 Pf4 BAC transgenes available for analysis also rescues the lethal FV null phenotype, with FV activity restricted to only the platelet pool (approximately 3% of the wild-type FV level). FV-null mice rescued by either the albumin or Pf4 BAC exhibit nearly normal tail bleeding times. These results demonstrate that Fv expression in either the platelet or plasma FV pool is sufficient for basal hemostasis. In addition, these findings indicate that the murine platelet and plasma FV pools are biosynthetically distinct, in contrast to a previous report demonstrating a plasma origin for platelet FV in humans.

scholarly journals Characterization of bacterial artificial chromosome transgenic mice expressing mCherry fluorescent protein substituted for the murine smooth muscle α-actin gene

genesis ◽  
2010 ◽  
Vol 48 (7) ◽  
pp. 457-463 ◽  
Author(s):  
John J. Armstrong ◽  
Irina V. Larina ◽  
Mary E. Dickinson ◽  
Warren E. Zimmer ◽  
Karen K. Hirschi
Keyword(s):  
Smooth Muscle ◽  
Bacterial Artificial Chromosome ◽  
Transgenic Mice ◽  
Fluorescent Protein ◽  
Artificial Chromosome ◽  
Actin Gene

Improved Pituitary Specificity of Cre Recombinase Transgenic Mice by Recombineering of a Mouse αGSU Bacterial Artificial Chromosome

2011 ◽  
pp. P1-383-P1-383
Author(s):  
Maria Ines Perez Millan ◽  
Sally A Camper ◽  
Shannon W Davis
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Transgenic Mice ◽  
Artificial Chromosome ◽  
Cre Recombinase

Corrigendum to “Distribution of estrogen receptor beta containing cells in the brains of bacterial artificial chromosome transgenic mice” [Brain Res. 1351 (2010) 74–96]

2011 ◽  
Vol 1396 ◽  
pp. 105
Author(s):  
Teresa A. Milner ◽  
Louisa I. Thompson ◽  
Gang Wang ◽  
Justin A. Kievits ◽  
Eugene Martin ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Bacterial Artificial Chromosome ◽  
Transgenic Mice ◽  
Estrogen Receptor Beta ◽  
Artificial Chromosome ◽  
Mice Brain ◽  
Receptor Beta

scholarly journals Distribution of estrogen receptor beta containing cells in the brains of bacterial artificial chromosome transgenic mice

2010 ◽  
Vol 1351 ◽  
pp. 74-96 ◽  
Author(s):  
Teresa A. Milner ◽  
Louisa I. Thompson ◽  
Gang Wang ◽  
Justin A. Kievits ◽  
Eugene Martin ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Bacterial Artificial Chromosome ◽  
Transgenic Mice ◽  
Estrogen Receptor Beta ◽  
Artificial Chromosome ◽  
Receptor Beta

Position-independent and tissue-specific expression of porcine whey acidic protein gene from a bacterial artificial chromosome in transgenic mice

2002 ◽  
Vol 63 (2) ◽  
pp. 161-167 ◽  
Author(s):  
Sylvie Rival-Gervier ◽  
C�line Viglietta ◽  
Caroline Maeder ◽  
Joe Attal ◽  
Louis-Marie Houdebine
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Transgenic Mice ◽  
Protein Gene ◽  
Artificial Chromosome ◽  
Whey Acidic Protein ◽  
Acidic Protein ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression
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