Geophysical investigations at the Anuru Bay trepang site: A new approach to locating Macassan archaeological sites in Northern Australia

This paper presents the results of a magnetometer survey and initial archaeological excavations of Macassan and Indigenous features conducted at the Anuru Bay Macassan trepang processing site. The archaeology of this area is complex, containing both material reflecting the Indigenous utilisation of coastal resources and the periodic visits of the Macassan trepangers from Indonesia. Despite a history of archaeological investigations on Macassan period sites (i.e. Clarke 1994; McKnight 1976; Mitchell 1994), geophysical survey has not previously been applied as part of these investigations. While Macassan sites may contain features amenable to conventional archaeological geophysics (such as iron trepang processing pots), additional potential exists for the application of magnetometry to locate features created through burning, as has been applied to Australian Indigenous sites (Bonhomme & Stanley 1985; Fanning et al. 2009; Moffat et al. 2008 & 2010; Stanley & Green 1976; Wallis et al. 2008) and international Indigenous sites (Abbot & Frederick 1990; Batt & Dockrill 1998; Jones & Munson 2005). The results of this study demonstrate that this approach is equally applicable to Macassan sites, opening up a new and potentially fruitful avenue for exploring the archaeology of this trade system.

Hierarchical length and sequence preferences establish a single major piRNA 3'-end

PIWI-interacting RNAs (piRNAs) guard germline genomes against the deleterious action of retroviruses and other mobile genetic elements. How piRNAs faithfully discriminate between self and non-self to restrict all mobile elements while sparing essential genes remains a key outstanding question in genome biology. PiRNAs use extensive base-pairing to recognize their targets and variable 3'ends could change the specificity and efficacy of piRNA silencing. Here, we identify conserved rules that ensure the generation of a single major piRNA 3'end in flies and mice. Our data suggest that the PIWI proteins initially define a short interval on pre-piRNAs that grants access to the ZUC-processor complex. Within this Goldilocks zone, the preference of the ZUC-processor to cut in front of a Uridine determines the ultimate processing site. We observe a mouse-specific roadblock that relocates the Goldilocks zone and generates an opportunity for consecutive trimming by PNLDC1. Our data reveal a conserved hierarchy between length and sequence preferences that controls the piRNA sequence space. The unanticipated precision of 3'end formation bolsters the emerging understanding that the functional piRNA sequence space is tightly controlled to ensure effective defense.

Retracing the evolution from antimicrobial to targeting peptides

We experimentally challenged the endosymbiotic hypothesis that organelle-targeting peptides derive from antimicrobial amphipathic peptides delivered by the host cell, to which organelle progenitors became resistant. To explore the molecular changes required to convert such antimicrobial peptides into bona fide organelle-targeting peptides, we expressed a set of 13 antimicrobial peptides of various origins in the green alga Chlamydomonas reinhardtii that serves as a model for both mitochondrial and chloroplast import. The peptides were modified to match distinctive features of mitochondrial and chloroplast targeting peptides, and we assessed their targeting potential by following the intracellular localization and maturation of a Venus fluorescent reporter used as cargo protein. We present a temporal evolutionary scenario that emphasizes the early contribution of exchanging Lysines with Arginines in the sequence of the antimicrobial peptide, the evolution of a processing site followed by the addition of unstructured sequence and protein interaction sites that allow the selective targeting to the chloroplast.

Determining Suitable Locations for Regional Waste Final Processing Site (WFP) Using the Simple Additive Weighting (SAW) (Case Study: Malang City and Malang Regency)

Garbage or waste is basically a residual material resulting from human activities and natural processes that have no economic value anymore. The volume of waste in Malang City and Malang Regency every year always increases, so the existing waste final processing site will no longer be able to accommodate the pile of garbage. Therefore the Malang City Government plans to collaborate with the Malang Regency Government in making an integrated regional waste landfill and processing site. In this study, an analysis of the determination of the appropriate location for regional waste final processing for Malang City and Malang Regency was carried out using the Simple Additive Weighting (SAW) method. The SAW method is a weighted summation method, which can make a more precise assessment, based on the predetermined criteria and preference weights. Preference weights were determined by pairwise comparison method. The criteria used are geological hazardous areas, distance from drinking water sources, land slope level, distance from settlements, protected areas and distance from airports. The final result in this study is a map of the appropriate location for a regional waste final processing site and an analysis of the location for the best regional final processing site. The location map is classified into three, namely: not feasible with a total area of 54,774.33 ha, less feasible with a total area of 170,846.49 ha and feasible with a total area of 130,096.63 ha.

Stephan Oroszlan and the Proteolytic Processing of Retroviral Proteins: Following A Pro

Steve Oroszlan determined the sequences at the ends of virion proteins for a number of different retroviruses. This work led to the insight that the amino-terminal amino acid of the mature viral CA protein is always proline. In this remembrance, we review Steve’s work that led to this insight and show how that insight was a necessary precursor to the work we have done in the subsequent years exploring the cleavage rate determinants of viral protease processing sites and the multiple roles the amino-terminal proline of CA plays after protease cleavage liberates it from its position in a protease processing site.

Social support role of occupational safety and health implementation in informal sector during COVID-19 pandemic

This study was conducted in a limestone processing site in Gunungkidul Regency, Yogyakarta, with One group pretest-posttest design. The study was initiated by OSH training for social supports done by competent health cadres. There were 8 social supports from fellow workers (peer support) and 29 social supports from family members (family support). These social supports assisted workers during 4 weeks. Data was collected by using three scales i.e. Scale of OSH knowledge (8 items, Coeficient Alpha Cronbach Reliability 0.800), attitude towards OSH scale (10 items, Reliability 0.917) and Practice of OSH Scale (10 items, Reliability 0.804). Data was collected before and after role assistance by social supports. Data analysis was done by the Wilcoxon sign rank test (alpha: 0.05). The results showed that there was an increase in the average knowledge of OSH from 7.77 to 8.89 (p<0.05), attitudes towards OSH from 26.23 to 30.76 (p<0.05), and OSH implementation from 25.00 to 28.83 (p< 0.05). It was proven that the involvement of social supports from peer as well as family could increase knowledge, attitude and OSH practice of limestone processing workers. To increase the accuracy of the study result, it is advisable that the further study use a control group.

Analysis of waste processing potential in Gunung Putri District, Bogor Regency

The increase in waste generation in Gunung Putri Subdistrict, Bogor Regency, causes an increased burden at the Landfill Site (TPA). One of the efforts to reduce the burden of Landfill Site (TPA) is to maximize the Waste Processing Site (TPS) by applying the circular economy concept. This study aims to analyze the generation, composition, and potential of waste processing to have economic value. The sampling method used is a combination of purposive sampling and stratified random sampling. The results showed that with a population of 304,672 people and a waste generation rate of 0.48 kg/person/day, it is estimated that the waste generation in Gunung Putri District is 147,156.58 kg/day. The waste material in Gunung Putri Subdistrict consists of 52.86% organic waste components and 47.14% non-organic waste. Organic waste is dominated by food waste by 50.38%. From the results of the characteristic test of the waste, it is known that the water content is 63.16%, the ash content is 29%, the C/N ratio is 7.03%, and the calorific value of the waste is 1.924 kcal/kg by considering the amount of generation, composition, and characteristics of waste, which are then used with standards or criteria for waste processing technology from various references. Organic waste in Gunung Putri Subdistrict has good potential to be processed using the Black Soldier Fly (BSF) and organic waste processing with Peuyeumisasi Method (Biodrying) and recycling for non-organic waste.

The policy of the Surakarta City Parks and Hygiene Service in environmental management

Based on Law Number 18 of 2008 concerning Waste Management, Tempat Pembuangan Sampah Akhir (TPA) is a Final Processing Site. The goal is to process and return waste to environmental media in a way that is safe for humans and the environment. The problems seen in the Putri Cempo TPA are the worrying land conditions, environmental pollution conditions, and poor management that the Government must pay attention to, because these have an impact on the surrounding community. This also caused a lot of complaints from the local community. The purpose of this study was to analyze the role of the Department of Parks and Hygiene in Waste Management in Surakarta City. In this study, researchers used descriptive qualitative research methods with 15 informants. The results showed that the management of the Putri Cempo TPA was not adequate, as seen from the many obstacles that occurred in Putri Cempo, such as several damaged vehicles and waste transportation equipment, the waste management operational system that determines the volume of waste disposed of in the TPA, and waste operational activities depending on the environment. operational pattern, namely the method of sweeping, collection, transportation, and final disposal.

Pengaruh Pandemi Covid-19 Terhadap Jumlah Sampah di Tempat Pemrosesan Akhir Regional Kota Payakumbuh

Restrictions on community activities during the Covid-19 pandemic have an impact on the amount of waste a city produces. This study aims to analyze the amount of incoming waste at the Payakumbuh Regional Final Processing Site (TPA) before and during the Covid-19 pandemic, analyze the impact of the pandemic on waste sources and provide recommendations for waste management during the pandemic. Data on the amount of waste entering the TPA was obtained from measurements of waste at the weighbridge before the pandemic (February – March 2020) and during the pandemic (April – May 2020). The analysis of the impact of the pandemic on the source of the waste is based on the results of interviews with truck drivers and analysis of truck routes. The results showed a decrease in the amount of waste transported to the landfill by 11.15% during the Covid-19 pandemic. The average amount of waste before the pandemic was 237.36 tons/day, while during the pandemic is 210.90 tons/day. The decrease in the amount of waste is due to restrictions on community activities outside the home with the implementation of the Large-Scale Social Restriction (PSBB) policy. The implementation of PSBB policy during the pandemic caused a decrease in the amount of waste from institutional, commercial and city service facilities by 29.48-50% and an increase in domestic sources by 21.22%.

Pemetaan dan Analisis Tempat Penampungan Sampah Sementara Menggunakan Sistem Informasi Geografis di Kecamatan Mataram, Kota Mataram

The transportation of waste from the Temporary Waste Shelter (TWS) to the Final Processing Site (FPS) needs to be carried out to avoid the accumulation of waste in the TWS, because it has the potential to endanger the environment and health. This study aims to determine the distribution, capacity, service radius, and technical feasibility of TWS using a Geographic Information System (GIS), and to determine the level of need for TWS facilities in the Mataram District. This research is a descriptive observational study, where the researchers conducted a field survey by means of observation, plotting coordinates with GPS, and measuring waste generation on the specified respondents. The data is processed and analyzed spatially and normatively, then described. The results showed that there were 8 points TWS in Mataram District, with an uneven distribution in each village, namely 3 wards that did not have a formal TWS. The capacity of the existing TWS is adequate for containing waste from its coverage service, as well as the condition of the TWS in general is good but they need to be repaired and maintained. The need TWS in Mataram District is 16 units where 8 of them have being existing. The remains for additional TWS is 8 units those can be placed in each village, especially in 3 villages that do not yet have TWS, namely Punia, Pagesangan Barat, and Pagesangan Timur