Generation of Bacterial Artificial Chromosome (BAC) Transgenic Mice

2014 ◽  
pp. 157-169 ◽  
Author(s):  
Jane Beil ◽  
Thorsten Buch
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Transgenic Mice ◽  
Artificial Chromosome ◽  
Bac Transgenic Mice

The murine platelet and plasma factor V pools are biosynthetically distinct and sufficient for minimal hemostasis

Blood ◽  
2003 ◽  
Vol 102 (8) ◽  
pp. 2856-2861 ◽  
Author(s):  
Hongmin Sun ◽  
Tony L. Yang ◽  
Angela Yang ◽  
Xixi Wang ◽  
David Ginsburg
Keyword(s):  
Gene Expression ◽  
Bacterial Artificial Chromosome ◽  
Transgenic Mice ◽  
Coagulation Factor ◽  
Artificial Chromosome ◽  
Coagulation Cascade ◽  
Factor V ◽  
Wild Type ◽  
Plasma Factor ◽  
Coagulation Factor V

Abstract Coagulation factor V (FV) is a central regulator of the coagulation cascade. Circulating FV is found in plasma and within platelet α granules. The specific functions of these distinct FV pools are uncertain. We now report the generation of transgenic mice with FV gene expression restricted to either the liver or megakaryocyte/platelet lineage using bacterial artificial chromosome (BAC) constructs. Six of 6 independent albumin BAC transgenes rescue the neonatal lethal hemorrhage of FV deficiency. Rescued mice all exhibit liver-specific Fv expression at levels ranging from 6% to 46% of the endogenous Fv gene, with no detectable FV activity within the platelet pool. One of the 3 Pf4 BAC transgenes available for analysis also rescues the lethal FV null phenotype, with FV activity restricted to only the platelet pool (approximately 3% of the wild-type FV level). FV-null mice rescued by either the albumin or Pf4 BAC exhibit nearly normal tail bleeding times. These results demonstrate that Fv expression in either the platelet or plasma FV pool is sufficient for basal hemostasis. In addition, these findings indicate that the murine platelet and plasma FV pools are biosynthetically distinct, in contrast to a previous report demonstrating a plasma origin for platelet FV in humans.

scholarly journals Characterization of bacterial artificial chromosome transgenic mice expressing mCherry fluorescent protein substituted for the murine smooth muscle α-actin gene

genesis ◽  
2010 ◽  
Vol 48 (7) ◽  
pp. 457-463 ◽  
Author(s):  
John J. Armstrong ◽  
Irina V. Larina ◽  
Mary E. Dickinson ◽  
Warren E. Zimmer ◽  
Karen K. Hirschi
Keyword(s):  
Smooth Muscle ◽  
Bacterial Artificial Chromosome ◽  
Transgenic Mice ◽  
Fluorescent Protein ◽  
Artificial Chromosome ◽  
Actin Gene

scholarly journals Distribution of estrogen receptor beta containing cells in the brains of bacterial artificial chromosome transgenic mice

2010 ◽  
Vol 1351 ◽  
pp. 74-96 ◽  
Author(s):  
Teresa A. Milner ◽  
Louisa I. Thompson ◽  
Gang Wang ◽  
Justin A. Kievits ◽  
Eugene Martin ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Bacterial Artificial Chromosome ◽  
Transgenic Mice ◽  
Estrogen Receptor Beta ◽  
Artificial Chromosome ◽  
Receptor Beta

A BACwards glance at neurodegeneration: molecular insights into disease from LRRK2, SNCA and MAPT BAC-transgenic mice

2011 ◽  
Vol 39 (4) ◽  
pp. 862-867 ◽  
Author(s):  
Sara J. Johnson ◽  
Richard Wade-Martins
Keyword(s):  
Transgenic Mice ◽  
Human Disease ◽  
Artificial Chromosome ◽  
Mutant Form ◽  
Genomic Locus ◽  
Disease States ◽  
Native Promoter ◽  
Wide Range ◽  
Type Gene ◽  
Bac Transgenic Mice

BAC (bacterial artificial chromosome)-transgenic mice expressing a transgene from an entire genomic locus under the control of the native promoter offer the opportunity to generate more accurate genetic models of human disease. The present review discusses results of recent studies investigating PD (Parkinson's disease) and tauopathies using BAC-transgenic mice carrying either the LRRK2 (leucine-rich repeat kinase 2), α-synuclein (SNCA) or MAPT (microtubule-associated protein tau) genes. In all lines, expression of the WT (wild-type) gene resulted in physiologically relevant protein expression. The effect of expressing the mutant form of a gene varied depending on the mouse strain or the particular disease mutation used, although it was common to see either neurochemical or behavioural differences in these animals. Overall, BAC technology offers an exciting opportunity to generate a wide range of new animal models of human-disease states.

scholarly journals The expression of human  -like globin genes in transgenic mice mediated by bacterial artificial chromosome

2001 ◽  
Vol 98 (26) ◽  
pp. 15073-15077 ◽  
Author(s):  
D.-X. Feng ◽  
D.-P. Liu ◽  
Y. Huang ◽  
L. Wu ◽  
T.-C. Li ◽  
...  
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Transgenic Mice ◽  
Artificial Chromosome ◽  
Globin Genes
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