scholarly journals Identification of ungulates used in a traditional Chinese medicine with DNA barcoding technology

2015 ◽  
Vol 5 (9) ◽  
pp. 1818-1825 ◽  
Author(s):  
Jing Chen ◽  
Zhigang Jiang ◽  
Chunlin Li ◽  
Xiaoge Ping ◽  
Shaopeng Cui ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Dna Barcoding ◽  
Barcoding Technology

scholarly journals Identifying the Species of Seeds in Traditional Chinese Medicine Using DNA Barcoding

2018 ◽  
Vol 9 ◽  
Author(s):  
Chao Xiong ◽  
Wei Sun ◽  
Jingjian Li ◽  
Hui Yao ◽  
Yuhua Shi ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Dna Barcoding

scholarly journals Use of rbcL sequences for DNA barcoding and authentication of plant drugs used in Traditional Chinese Medicine

2014 ◽  
Author(s):  
Florian Herrmann ◽  
Michael Wink
Keyword(s):  
Quality Control ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Dna Barcoding ◽  
Marker Gene ◽  
Fast Method ◽  
Chemical Profiling ◽  
Wrong Identification ◽  
Rbcl Sequences ◽  
Universal Application

Traditional Chinese medicine has become increasingly popular in Europe and North America.There is evidence that quality control in terms of species authentication is sometimes inappropriate. Repeated incidents of adulterations and wrong identification, some even with serious consequences have occurred recently. The necessity of a quality control for TCM drugs to avoid these incidents is given since many years. DNA barcoding was used in this study to authenticate drugs which are often used in Chinese herbal medicine. 37 plants from 28 families were identified using nucleotide sequences of the rbcL gene. Only one adulteration could be detected. Both the advantages and limitations of rbcL as a marker gene for identification were analysed and discussed. We could show that DNA barcoding is a valid and fast method to identify medicinal herbs, showing some advantages over chemical profiling because of its universal application even for unknown plant species.

scholarly journals Use of rbcL sequences for DNA barcoding and authentication of plant drugs used in Traditional Chinese Medicine

2014 ◽  
Author(s):  
Florian Herrmann ◽  
Michael Wink
Keyword(s):  
Quality Control ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Dna Barcoding ◽  
Marker Gene ◽  
Fast Method ◽  
Chemical Profiling ◽  
Wrong Identification ◽  
Rbcl Sequences ◽  
Universal Application

Traditional Chinese medicine has become increasingly popular in Europe and North America.There is evidence that quality control in terms of species authentication is sometimes inappropriate. Repeated incidents of adulterations and wrong identification, some even with serious consequences have occurred recently. The necessity of a quality control for TCM drugs to avoid these incidents is given since many years. DNA barcoding was used in this study to authenticate drugs which are often used in Chinese herbal medicine. 37 plants from 28 families were identified using nucleotide sequences of the rbcL gene. Only one adulteration could be detected. Both the advantages and limitations of rbcL as a marker gene for identification were analysed and discussed. We could show that DNA barcoding is a valid and fast method to identify medicinal herbs, showing some advantages over chemical profiling because of its universal application even for unknown plant species.

scholarly journals Application of DNA Barcoding for the Identification of a Traditional Chinese Medicine Shedan

2021 ◽  
Author(s):  
Chenggao Zhou ◽  
Suran Gan ◽  
Jie Zhang ◽  
Yeqin Fan ◽  
Bin Li ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Dna Barcoding ◽  
Universal Primers ◽  
Specific Primers ◽  
Original Source ◽  
Snake Species ◽  
History Of ◽  
Origin Identification ◽  
Bile Samples

Abstract Background Shedan has a long history of application in Traditional Chinese medicine (TCM), however, Shedan from different original source has been indiscriminately used. So far, there is still a lack of an effective tool to differentiate the original source of Shedan medicinal materials, which brings great risk to the safety and effectiveness of clinical applications. Hence, it is imperative to develop a practicable approach to identify Shedan medicinal materials. Methods The specificity of two pairs of primers, including Folmer’s universal primers and a pair of originally designed primers COISNFF/COISNFR, was tested to screen the more specific primers for further origin identification of Shedan. A total of 253 fresh snake gallbladder samples from 31 morphologically identified snake species were collected and authenticated. Moreover, 51 fresh snake bile samples and 17 fresh bile samples from five other common domestic poultry and livestock (cattle, chicken, duck, pig and sheep) were collected and distinguished using the more specific primers. Additionally, a total of 195 market Shedan samples randomly selected from 18 batches of Shedan medicinal materials were investigated. Sequence definition was executed by querying sequence similarities in GenBank and the Barcode of Life Data System (BOLD), respectively. Results It turned out that the standard COI barcode obtained by COISNFF/COISNFR primers, rather than Folmer’s universal primers, can distinguish all the testing samples from each other in fresh Shedan samples, and COISNFF/COISNFR primers were also specific to snake species and the other four animal species except duck. In terms of market Shedan, 84.6% (165/195) samples can be attributed to 13 snake species from four families and 4.6% (9/195) can be attributed to adulterated chicken species. Conclusion The COI-based DNA barcoding was practicable for species identification of Shedan used in traditional Chinese medicine. The original source of current market Shedan, including adulterated species, has been preliminarily clarified, which provides a foundation for quality control of Shedan medicinal materials.

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