scholarly journals Application of DNA Barcoding for the Identification of a Traditional Chinese Medicine Shedan

2021 ◽  
Author(s):  
Chenggao Zhou ◽  
Suran Gan ◽  
Jie Zhang ◽  
Yeqin Fan ◽  
Bin Li ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Dna Barcoding ◽  
Universal Primers ◽  
Specific Primers ◽  
Original Source ◽  
Snake Species ◽  
History Of ◽  
Origin Identification ◽  
Bile Samples

Abstract Background Shedan has a long history of application in Traditional Chinese medicine (TCM), however, Shedan from different original source has been indiscriminately used. So far, there is still a lack of an effective tool to differentiate the original source of Shedan medicinal materials, which brings great risk to the safety and effectiveness of clinical applications. Hence, it is imperative to develop a practicable approach to identify Shedan medicinal materials. Methods The specificity of two pairs of primers, including Folmer’s universal primers and a pair of originally designed primers COISNFF/COISNFR, was tested to screen the more specific primers for further origin identification of Shedan. A total of 253 fresh snake gallbladder samples from 31 morphologically identified snake species were collected and authenticated. Moreover, 51 fresh snake bile samples and 17 fresh bile samples from five other common domestic poultry and livestock (cattle, chicken, duck, pig and sheep) were collected and distinguished using the more specific primers. Additionally, a total of 195 market Shedan samples randomly selected from 18 batches of Shedan medicinal materials were investigated. Sequence definition was executed by querying sequence similarities in GenBank and the Barcode of Life Data System (BOLD), respectively. Results It turned out that the standard COI barcode obtained by COISNFF/COISNFR primers, rather than Folmer’s universal primers, can distinguish all the testing samples from each other in fresh Shedan samples, and COISNFF/COISNFR primers were also specific to snake species and the other four animal species except duck. In terms of market Shedan, 84.6% (165/195) samples can be attributed to 13 snake species from four families and 4.6% (9/195) can be attributed to adulterated chicken species. Conclusion The COI-based DNA barcoding was practicable for species identification of Shedan used in traditional Chinese medicine. The original source of current market Shedan, including adulterated species, has been preliminarily clarified, which provides a foundation for quality control of Shedan medicinal materials.

scholarly journals Composition of “gold juice” using an ancient method based on intestinal microecology

2020 ◽  
Vol 48 (9) ◽  
pp. 030006052093128
Author(s):  
Qiuwei Li ◽  
Liying Guo ◽  
Li Wang ◽  
Jing Miao ◽  
Huantian Cui ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Fecal Microbiota Transplantation ◽  
Antibiotic Use ◽  
Fecal Microbiota ◽  
Healthy Children ◽  
Fecal Samples ◽  
16S Rdna Sequence Analysis ◽  
History Of ◽  
Chinese Medicine Treatment

Objective To identify potentially effective bacterial components of gold juice, a traditional Chinese medicine treatment used for fecal microbiota transplantation. Methods Fecal samples were collected from five healthy children (two boys and three girls; mean age, 7.52 ± 2.31 years). The children had no history of antibiotic use or intestinal microecological preparation in the preceding 3 months. Fresh fecal samples were collected from children to prepare gold juice in mid-to-late November, in accordance with traditional Chinese medicine methods, then used within 7 days. Finally, 16S rDNA sequence analysis was used to identify potentially effective bacterial components of gold juice. QIIME software was used for comparisons of microbial species among gold juice, diluent, filtrate, and loess samples. Results Microflora of gold juice exhibited considerable changes following “ancient method” processing. Microbial components significantly differed between gold juice and filtrate samples. The gold juice analyzed in our study consisted of microbes that synthesize carbohydrates and amino acids by degrading substances, whereas the filtrate contained probiotic flora, Bacteroides, and Prevotella 9. Conclusions This study of microbial components in gold juice and filtrate provided evidence regarding effective bacterial components in gold juice, which may aid in clinical decisions concerning fecal microbiota transplantation.

scholarly journals From Prejudice to Evidence: The Case of Rhizoma Coptidis in Singapore

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Chin Ee Ho ◽  
You Li Goh ◽  
Chang Zhang
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
G6pd Deficiency ◽  
Neonatal Jaundice ◽  
Therapeutic Effects ◽  
Evidence Based ◽  
Rhizoma Coptidis ◽  
History Of ◽  
Hepatoprotective Effects ◽  
Glucose 6 Phosphate Dehydrogenase

Rhizoma Coptidis (RC), commonly known ashuanglian, is a herb frequently used in Traditional Chinese Medicine (TCM) prescriptions. Known to have “clearing damp-heat, quenching fire and counteracting poison” properties, it was widely used in the Chinese community in Singapore. Berberine, an alkaloid isolated from RC, is known to have a wide array of therapeutic effects including antimicrobial, antineoplastic, and hepatoprotective effects. In 1978, RC was implicated in causing neonatal jaundice (NNJ) and kernicterus in neonates suffering from glucose-6-phosphate dehydrogenase (G6PD) deficiency, leading to the banning of RC and berberine in Singapore. More than three decades later, accumulating evidence-based studies pointing to the safety of RC for general public and better understanding of G6PD deficiency, the Health Sciences Authority (HSA) in Singapore reviewed and lifted the prohibition on RC and berberine, turning a brand new chapter in the history of TCM in Singapore. This paper aims to review the safety of RC and berberine, using the prohibition of use and subsequent lifting of ban on RC and berberine in Singapore as an illustration to highlight the importance of evidence-based studies in Traditional Chinese Medicine (TCM).

Travel and Transformation

2017 ◽  
Vol 12 (1) ◽  
pp. 1-21
Author(s):  
Bo Hu
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Medical History ◽  
Public Discourse ◽  
Nation Building ◽  
Journal Articles ◽  
History Of

Few concepts in the medical history of China have been more entangled with modernization and nation-building than weisheng. Originally a concept inextricably bound up with traditional Chinese medicine, it underwent drastic changes and became a near equivalent of modern hygiene. Drawing on the notion of traveling concepts, this article traces the travel and transformation it experienced in journal articles between 1880 and 1930, focusing on how the new concept gradually took shape and became established in public discourse, the enabling and resisting agents, as well as their agendas.

scholarly journals Identifying the Species of Seeds in Traditional Chinese Medicine Using DNA Barcoding

2018 ◽  
Vol 9 ◽  
Author(s):  
Chao Xiong ◽  
Wei Sun ◽  
Jingjian Li ◽  
Hui Yao ◽  
Yuhua Shi ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Dna Barcoding

Gag Reflex Control through Acupuncture: A Case Series

2014 ◽  
Vol 32 (1) ◽  
pp. 24-27 ◽  
Author(s):  
Giuseppa Bilello ◽  
Antonella Fregapane
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Visual Analogue Scale ◽  
Analogue Scale ◽  
Case Series ◽  
Inclusion Criteria ◽  
History Of ◽  
Gag Reflex ◽  
Reflex Control ◽  
Dental Appointments

Background Many patients avoid dental appointments because of severe gagging when they have work performed, such as taking impressions. There are several methods known to alleviate gagging, and some studies have suggested that acupuncture may be effective in reducing gag reflex. The aim of the present study therefore was to evaluate whether acupuncture can produce a reduction of the gag reflex. Methods A total of 20 patients, aged between 19 and 80 years, with history of gag reflex on taking dental impressions, were recruited. All patients fulfilling the inclusion criteria had an upper and lower dental alginate impression taken without acupuncture, and a second upper and lower alginate impression taken immediately after acupuncture based on traditional Chinese medicine. After each impression, the patients recorded their emetic sensation using a visual analogue scale (VAS). Results The results showed a significant (p<0.05) reduction in the gag reflex scores after acupuncture. For upper impressions, they fell from 6.8 (1.1) to 1.1 (1.1); and for lower impressions, from 5.45 (1.0) to 0.4 (0.7) (mean (SD)). Conclusions The findings from our study suggest that acupuncture may be useful for preventing and treating gag reflex, and justifies further study.

scholarly journals Lonicerae Japonicae Flos and Lonicerae Flos: A Systematic Pharmacology Review

2015 ◽  
Vol 2015 ◽  
pp. 1-16 ◽  
Author(s):  
Yujie Li ◽  
Weiyan Cai ◽  
Xiaogang Weng ◽  
Qi Li ◽  
Yajie Wang ◽  
...  
Keyword(s):  
Systematic Review ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Traditional Medicine ◽  
Pharmacological Study ◽  
Research Data ◽  
Academic Journals ◽  
Clinical Safety ◽  
History Of ◽  
Pharmacology And Toxicology

Lonicerae japonicae flos, a widely used traditional Chinese medicine (TCM), has been used for several thousand years in China.Chinese Pharmacopeiaonce included Lonicerae japonicae flos of Caprifoliaceae family and plants of the same species named Lonicerae flos in general in the same group.Chinese Pharmacopeia(2005 Edition) lists Lonicerae japonicae flos and Lonicerae flos under different categories, although they have the similar history of efficacy. In this study, we research ancient books of TCM, 4 main databases of Chinese academic journals, and MEDLINE/PubMed to verify the origins and effects of Lonicerae japonicae flos and Lonicerae flos in traditional medicine and systematically summarized the research data in light of modern pharmacology and toxicology. Our results show that Lonicerae japonicae flos and Lonicerae flos are similar pharmacologically, but they also differ significantly in certain aspects. A comprehensive systematic review and a standard comparative pharmacological study of Lonicerae japonicae flos and Lonicerae flos as well as other species of Lonicerae flos support their clinical safety and application. Our study provides evidence supporting separate listing of Lonicerae japonicae flos and Lonicerae flos inChinese Pharmacopeiaas well as references for revision of relevant pharmacopeial records dealing with traditional efficacy of Lonicerae japonicae flos and Lonicerae flos.

scholarly journals Meraque/San Jiao of Eastern Medicine in Comparison with the Mesentery System Presented in Western Medicine

2017 ◽  
Vol 4 (3-4) ◽  
pp. 101-106 ◽  
Author(s):  
Majid Avijgan ◽  
Mahtab Avijgan ◽  
Firouze Salehzadeh
Keyword(s):  
Adipose Tissue ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Western Medicine ◽  
Integrated System ◽  
Previous Article ◽  
New Methods ◽  
Persian Medicine ◽  
History Of ◽  
New System

Background: In Persian medicine (PM) and traditional Chinese medicine (TCM), there is an ignored space, namely the Meraque/San Jiao (M/S) space, which in our previous article has been described in detail. Recently, there has been a publication about the mesentery system describing it as a new system with a history of no more than 300 years. According to our previous publications, this is a part of the M/S system, which could represent adipose tissue and vessels. The mesentery and the M/S system can be regarded as one system, except that the mesentery system is a section of the integrated system of M/S with a history of more than 3,000 years; this is the main topic of this report. Key Message: Comparing the mesentery with the Meraque in PM and the San Jiao in TCM, we can conclude that the M/S is more complete than the mesentery. Based on ancient classical texts of PM, the Meraque system has 6 labyrinths and includes all membranes, the fascia, vessels, adipose tissue, and the gut. In PM and TCM, it has a very important role. We will attempt to modernize the M/S. This leads to new methods in treatment, management, and research of Western medicine.

Study on the history of Traditional Chinese Medicine to treat diabetes

2010 ◽  
Vol 2 (1) ◽  
pp. 41-46 ◽  
Author(s):  
Hui Zhang ◽  
Conge Tan ◽  
Hongzhan Wang ◽  
Shengbo Xue ◽  
Miqu Wang
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
History Of

scholarly journals Strychnine poisoning due to traditional Chinese medicine: a case series

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 924
Author(s):  
Hok-Fung Tong ◽  
Candace Yim Chan ◽  
Sau-Wah Ng ◽  
Tony Wing-Lai Mak
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Clinical Features ◽  
Drugs Of Abuse ◽  
Case Series ◽  
Case Fatality ◽  
Reference Laboratory ◽  
History Of ◽  
Different Origins ◽  
Different Parts

Background: Strychnine poisoning is rare but possibly fatal. The most reported sources of strychnine poisoning include rodenticides and adulterated street heroin. Here we report a case series of an unusual cause of strychnine poisoning – Strychni semen, a herb known as “maqianzi” in traditional Chinese medicine (TCM). Methods: All cases of strychnine poisoning confirmed by the Hospital Authority Toxicology Reference Laboratory (HATRL, the highest-level clinical toxicology laboratory in Hong Kong) between May 2005 and May 2018 were reviewed. Results: Twelve cases of strychnine poisoning were recorded, and Strychni semen was the exclusive source. Ten (83%) patients presented with muscle spasms, and four (33%) developed typical conscious convulsions. The poisoning was severe in two (17%) patients, moderate in three (25%) and mild in eight (58%). No case fatality was recorded. Three (25%) patients were TCM practitioners and two (17%) were laymen who bought the herb themselves without a proper prescription. Conclusion: The practice of TCM is becoming popular in different parts of the world amid the COVID-19 pandemic. The spectrum of clinical features of strychnine poisoning secondary to Strychni semen are similar to those arising from different origins. Eliciting a history of TCM use, apart from exposure to rodenticides and drugs of abuse, may allow timely diagnosis in patients with compatible clinical features. Enhancement of TCM safety could minimize the hazard.

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