Application and comparison of high-speed countercurrent chromatography and high-performance liquid chromatography in semi-preparative separation of decarboxymethyl oleuropein aglycone (3,4-DHPEA-EDA), a bioactive secoiridoid from extra-virgin olive oil

2016 ◽  
Vol 119 (2) ◽  
pp. 1500532 ◽  
Author(s):  
Cecilia Jiménez-Sánchez ◽  
Jesús Lozano-Sánchez ◽  
Margret Brüggemann ◽  
Mariana Neves-Vieira ◽  
Miriam Rodriguez-Werner ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Olive Oil ◽  
High Speed ◽  
High Performance ◽  
Virgin Olive Oil ◽  
Extra Virgin Olive Oil ◽  
Countercurrent Chromatography ◽  
Preparative Separation

scholarly journals In House Validated UHPLC Protocol for the Determination of the Total Hydroxytyrosol and Tyrosol Content in Virgin Olive Oil Fit for the Purpose of the Health Claim Introduced by the EC Regulation 432/2012 for “Olive Oil Polyphenols”

Molecules ◽  
2019 ◽  
Vol 24 (6) ◽  
pp. 1044 ◽  
Author(s):  
Maria Tsimidou ◽  
Michaela Sotiroglou ◽  
Aspasia Mastralexi ◽  
Nikolaos Nenadis ◽  
Diego García-González ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Olive Oil ◽  
High Performance ◽  
Virgin Olive Oil ◽  
Cost Effective ◽  
Health Claim ◽  
Relative Standard ◽  
Oil Sector ◽  
Hydrolysis Conditions

An ongoing challenge in olive oil analytics is the development of a reliable procedure that can draw the consensus of all interested parties regarding the quantification of concentrations above the required minimum value of 5 mg of bioactive “olive oil polyphenols” per 20 g of the oil, to fulfill the health claim introduced by the European Commission (EC) Regulation 432/2012. An in-house validated ultra-high performance liquid chromatography (UHPLC) protocol fit for this purpose is proposed. It relies on quantification of the total hydroxytyrsol (Htyr) and tyrosol (Tyr) content in the virgin olive oil (VOO) polar fraction (PF) before and after acidic hydrolysis of their bound forms. PF extraction and hydrolysis conditions were as previously reported. The chromatographic run lasts ~1/3 of the time needed under high performance liquid chromatography (HPLC) conditions, this was also examined. Eluent consumption for the same piece of information was 6-fold less. Apart from being cost effective, a larger number of samples can be analyzed daily with less environmental impact. Two external curves, detection at 280 nm and correction factors for molecular weight difference are proposed. The method, which is fit for purpose, is selective, robust with satisfactory precision (percentage relative standard deviation (%RSD) values < 11%) and recoveries higher than 87.6% for the target analytes (Htyr, Tyr). Standard operational procedures are easy to apply in the olive oil sector.

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