Identification of mouse brain proteins after two-dimensional electrophoresis and electroblotting by microsequence analysis and amino acid composition analysis

1988 ◽  
Vol 9 (12) ◽  
pp. 830-838 ◽  
Author(s):  
Christoph Eckerskorn ◽  
Peter Jungblut ◽  
Werner Mewes ◽  
Joachim Klose ◽  
Friedrich Lottspeich
Keyword(s):  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Mouse Brain ◽  
Composition Analysis ◽  
Two Dimensional ◽  
Brain Proteins ◽  
Two Dimensional Electrophoresis ◽  
Amino Acid Composition Analysis ◽  
Dimensional Electrophoresis

Identification of polypeptides on two-dimensional electrophoresis gels by amino acid composition.

1984 ◽  
Vol 30 (12) ◽  
pp. 1925-1932 ◽  
Author(s):  
G I Latter ◽  
S Burbeck ◽  
J Fleming ◽  
J Leavitt
Keyword(s):  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Human Cell Line ◽  
Two Dimensional ◽  
Data Bases ◽  
Computer Data ◽  
Tentative Identification ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis

Abstract We present a method that can, in principle, provide tentative identification of a substantial proportion of the polypeptides resolvable on a given two-dimensional electrophoresis gel. Computerized microdensitometry of autoradiograms from 20 gels labeled in turn with each of the 20 common amino acids provides the data for simultaneously measuring the amino acid composition of all polypeptides of interest on the gel. These compositions are then compared with computer data bases of known protein compositions. Similarity between a known and an unknown polypeptide with comparable molecular mass indicates a potential identification, which can then be confirmed with conventional techniques. We illustrate this technique by applying it to the identification of proteins in a transformed human cell line (HuT-14).

scholarly journals Comparative codon and amino acid composition analysis of Tritryps-conspicuous features ofLeishmania major

FEBS Letters ◽  
2007 ◽  
Vol 581 (30) ◽  
pp. 5751-5758 ◽  
Author(s):  
Ipsita Chanda ◽  
Archana Pan ◽  
Sanjoy Kumar Saha ◽  
Chitra Dutta
Keyword(s):  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Composition Analysis ◽  
Amino Acid Composition Analysis
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