Hydrolysis and amino acid composition analysis of proteins

1998 ◽  
Vol 826 (2) ◽  
pp. 109-134 ◽  
Author(s):  
Michael Fountoulakis ◽  
Hans-Werner Lahm
FEBS Letters ◽  
2007 ◽  
Vol 581 (30) ◽  
pp. 5751-5758 ◽  
Author(s):  
Ipsita Chanda ◽  
Archana Pan ◽  
Sanjoy Kumar Saha ◽  
Chitra Dutta

2000 ◽  
Vol 28 (6) ◽  
pp. 589-590
Author(s):  
H. Okuyama ◽  
S. Hagiwara ◽  
T. Okamoto ◽  
K. Kato

A suspension culture of the moss Marchantia polymorpha was incubated with either [3H]-ethanolamine or [3H]myristic acid, precursors of a glycosylphosphatidylinositol (GPI) anchor. By both of these a secretory protein of 47 kDa was consistently labelled. Amino acid composition analysis of the chromatographically purified 47-kDa protein exhibited the occurrence of ethanolamine and glucosamine at approximate molar ratios to protein of 1 and 4, respectively. Thus the secretory 47-kDa protein of M. polymorpha was concluded to be a GPI-anchored protein.


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