Tissue engineering with electric fields: Investigation of the shape of mammalian cell aggregates formed at interdigitated oppositely castellated electrodes

2007 ◽  
Vol 28 (21) ◽  
pp. 3821-3828 ◽  
Author(s):  
Anil Sebastian ◽  
Alagarswamy G. Venkatesh ◽  
Gerard H. Markx
Keyword(s):  
Tissue Engineering ◽  
Mammalian Cell ◽  
Electric Fields ◽  
Cell Aggregates

In vivo study of the angiogenesis potential of bone marrow-derived mesenchymal stem cell aggregates in their niche like environment

2021 ◽  
pp. 039139882110255
Author(s):  
Sara Anajafi ◽  
Azam Ranjbar ◽  
Monireh Torabi-Rahvar ◽  
Naser Ahmadbeigi
Keyword(s):  
Tissue Engineering ◽  
Bone Marrow ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cultured Cells ◽  
Morphological Evidence ◽  
Cell Aggregates ◽  
Plla Scaffold ◽  
Significant Difference

Background: Sufficient blood vessel formation in bioengineered tissues is essential in order to keep the viability of the organs. Impaired development of blood vasculatures results in failure of the implanted tissue. The cellular source which is seeded in the scaffold is one of the crucial factors involved in tissue engineering methods. Materials and methods: Considering the notable competence of Bone Marrow derived Mesenchymal Stem Cell aggregates for tissue engineering purposes, in this study BM-aggregates and expanded BM-MSCs were applied without any inductive agent or co-cultured cells, in order to investigate their own angiogenesis potency in vivo. BM-aggregates and BM-MSC were seeded in Poly-L Lactic acid (PLLA) scaffold and implanted in the peritoneal cavity of mice. Result: Immunohistochemistry results indicated that there was a significant difference ( p < 0.050) in CD31+ cells between PLLA scaffolds contained cultured BM-MSC; PLLA scaffolds contained BM-aggregates and empty PLLA. According to morphological evidence, obvious connections with recipient vasculature and acceptable integration with surroundings were established in MSC and aggregate-seeded scaffolds. Conclusion: Our findings revealed cultured BM-MSC and BM-aggregates, capacity in order to develop numerous connections between PLLA scaffold and recipient’s vasculature which is crucial to the survival of tissues, and considerable tendency to develop constructs containing CD31+ endothelial cells which can contribute in vessel’s tube formation.

Poly(3-hydroxybutyrate-co-valerate)/poly(3-thiophene ethyl acetate) blends as a electroactive biomaterial substrate for tissue engineering application

RSC Advances ◽  
2016 ◽  
Vol 6 (30) ◽  
pp. 25330-25338 ◽  
Author(s):  
M. S. Recco ◽  
A. C. Floriano ◽  
D. B. Tada ◽  
A. P. Lemes ◽  
R. Lang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Proliferation ◽  
Ethyl Acetate ◽  
Mammalian Cell ◽  
Engineering Application ◽  
Chemical Properties ◽  
Tissue Engineering Application ◽  
Good Adhesion ◽  
Physical Chemical ◽  
Low Cytotoxicity

Polyblend films based on poly(3-hydroxybutirate-co-valerate) and poly(3-thiophene ethyl acetate) – PHBV/PTAcEt showed low cytotoxicity, good adhesion and mammalian cell proliferation. The physical–chemical properties were explored.

In vitro self-assembly of proepicardial cell aggregates: An embryonic vasculogenic model for vascular tissue engineering

2006 ◽  
Vol 288A (7) ◽  
pp. 700-713 ◽  
Author(s):  
José M. Pérez-Pomares ◽  
V. Mironov ◽  
Juan A. Guadix ◽  
David Macías ◽  
Roger R. Markwald ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Self Assembly ◽  
Vascular Tissue ◽  
Vascular Tissue Engineering ◽  
Cell Aggregates

scholarly journals 3D-printing enabled micro-assembly of a microfluidic electroporation system for 3D tissue engineering

Lab on a Chip ◽  
2019 ◽  
Vol 19 (14) ◽  
pp. 2362-2372 ◽  
Author(s):  
Qingfu Zhu ◽  
Megan Hamilton ◽  
Bryan Vasquez ◽  
Mei He
Keyword(s):  
Tissue Engineering ◽  
Cell Growth ◽  
3D Printing ◽  
Electric Fields ◽  
Micro Assembly ◽  
Cellular Transfection

The LEGO® concept is used to build 3D microchannel networks as a 3D μ-electrotransfection system for efficient exchange of nutrition and waste allowing 3D cell growth, while sustaining uniform 3D electric fields during cellular transfection.

Impedimetric Characterization of Bioelectronic Nano-Antennae

2020 ◽  
Author(s):  
Andie Robinson ◽  
Akhil Jain ◽  
Ruman Rahman ◽  
Sidahmed Abayzeed ◽  
Richard Hague ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Electric Fields ◽  
Rational Design ◽  
Au Nanoparticle ◽  
Drive Current ◽  
Cellular Behavior ◽  
Bipolar Electrodes ◽  
Electrochemical Systems ◽  
Bioelectronic Medicine

<p>The merging of electronics with biology at the nanoscale holds considerable promise for sensing and modulating cellular behavior. Advancing our understanding of nano-bioelectronics will facilitate development and enable applications in biosensing, tissue engineering and bioelectronic medicine. However, studies investigating the electrical effects when merging wireless conductive nanoelectrodes with biology are lacking. Consequently, a new tool is required to develop a greater understanding of the bioelectrical effects of merging conductive nanoparticles with biology. Herein, this challenge is addressed by developing an impedimetric method to evaluate bipolar electrochemical systems (BESs) that could act as nano-antennas. A theoretical framework is provided, using impedance to determine if conductive nanoparticles can be polarized and used to drive current. It is then demonstrated that 125 nm Au nanoparticle bipolar electrodes (BPEs) could be sensed in the presence of biology when incorporated intracellularly at 500 mg/ml, using water and PBS as electrolytes. These results highlight how nanoscale BPEs act within biological systems and characterize their behavior in electric fields. This research will impact on the rational design of using BPE systems in biology for both sensing and actuating applications.</p>

Impedimetric Characterization of Bioelectronic Nano-Antennae

2020 ◽  
Author(s):  
Andie Robinson ◽  
Akhil Jain ◽  
Ruman Rahman ◽  
Sidahmed Abayzeed ◽  
Richard Hague ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Electric Fields ◽  
Rational Design ◽  
Au Nanoparticle ◽  
Drive Current ◽  
Cellular Behavior ◽  
Bipolar Electrodes ◽  
Electrochemical Systems ◽  
Bioelectronic Medicine

<p>The merging of electronics with biology at the nanoscale holds considerable promise for sensing and modulating cellular behavior. Advancing our understanding of nano-bioelectronics will facilitate development and enable applications in biosensing, tissue engineering and bioelectronic medicine. However, studies investigating the electrical effects when merging wireless conductive nanoelectrodes with biology are lacking. Consequently, a new tool is required to develop a greater understanding of the bioelectrical effects of merging conductive nanoparticles with biology. Herein, this challenge is addressed by developing an impedimetric method to evaluate bipolar electrochemical systems (BESs) that could act as nano-antennas. A theoretical framework is provided, using impedance to determine if conductive nanoparticles can be polarized and used to drive current. It is then demonstrated that 125 nm Au nanoparticle bipolar electrodes (BPEs) could be sensed in the presence of biology when incorporated intracellularly at 500 mg/ml, using water and PBS as electrolytes. These results highlight how nanoscale BPEs act within biological systems and characterize their behavior in electric fields. This research will impact on the rational design of using BPE systems in biology for both sensing and actuating applications.</p>

Sign in / Sign up

Export Citation Format

Share Document