Enzyme biosensor based on pH‐sensitive field‐effect transistors for assessment of total indole alkaloids content in tissue culture of Rauwolfia serpentina

A laboratory prototype of an enzyme biosensor based on pH‑sensitive field-effect transistors has been developed to determine the total content of indole alkaloids in Rauwolfia serpentina Benth. Ex Kurz tissue culture. The biosensor was characterized by high sensitivity to th A laboratory prototype of an enzyme biosensor based on pH‑sensitive field effect transistors has been developed to determine the total content of indole alkaloids in Rauwolfia serpentina Benth. Ex Kurz tissue culture. The biosensor was characterized by high sensitivity to the total content of indole alkaloids (minimum limit of determination – 0.5 μg/ml of the total content of indole alkaloids contained in the juice obtained from tissue culture of Rauwolfia serpentina). The linear range of biosensor determination of the analyte was from 2 to 15 μg / ml of the total content of indole alkaloids. Analysis of indole alkaloids using a biosensor is simple and fast and does not require expensive equipment and special sample preparation for analysis, unlike traditional methods. The created biosensor can be further used to control the total content of indole alkaloids in modern biotechnological and pharmaceutical processes for the production of drugs and biologically active additives. e total content of indole alkaloids (minimum limit of determination – 0.5 μg/ml of the total content of indole alkaloids contained in the juice obtained from tissue culture of Rauwolfia serpentina). The linear range of biosensor determination of the analyte was from 2 to 15 μg / ml of the total content of indole alkaloids. Analysis of indole alkaloids using a biosensor is simple and fast and does not require expensive equipment and special sample preparation for analysis, unlike traditional methods. The created biosensor can be further used to control the total content of indole alkaloids in modern biotechnological and pharmaceutical processes for the production of drugs and biologically active additives.

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A Novel Enzyme Biosensor Specific for Formaldehyde Based on pH-Sensitive Field Effect Transistors

Journal of Chemical Technology & Biotechnology ◽

10.1002/(sici)1097-4660(199702)68:2<209::aid-jctb609>3.0.co;2-3 ◽

1997 ◽

Vol 68 (2) ◽

pp. 209-213 ◽

Cited By ~ 22

Author(s):

Yaroslav I. Korpan ◽

Alexey P. Soldatkin ◽

Mikhailo V. Gonchar ◽

Andrii A. Sibirny ◽

Timothy D. Gibson ◽

...

Keyword(s):

Field Effect ◽

Field Effect Transistors ◽

Ph Sensitive ◽

Enzyme Biosensor

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A novel enzyme biosensor for steroidal glycoalkaloids detection based on pH-sensitive field effect transistors

Bioelectrochemistry ◽

10.1016/s1567-5394(01)00150-5 ◽

2002 ◽

Vol 55 (1-2) ◽

pp. 9-11 ◽

Cited By ~ 24

Author(s):

Y.I Korpan ◽

V.V Volotovsky ◽

C Martelet ◽

N Jaffrezic-Renault ◽

E.A Nazarenko ◽

...

Keyword(s):

Field Effect ◽

Field Effect Transistors ◽

Ph Sensitive ◽

Enzyme Biosensor

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Development of enzyme biosensor based on pH-sensitive field-effect transistors for detection of phenolic compounds

Bioelectrochemistry ◽

10.1016/s1567-5394(01)00165-7 ◽

2002 ◽

Vol 55 (1-2) ◽

pp. 79-81 ◽

Cited By ~ 24

Author(s):

S.V Dzyadevych ◽

T Mai Anh ◽

A.P Soldatkin ◽

N Duc Chien ◽

N Jaffrezic-Renault ◽

...

Keyword(s):

Phenolic Compounds ◽

Field Effect ◽

Field Effect Transistors ◽

Ph Sensitive ◽

Enzyme Biosensor

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pH-sensitive diamond field-effect transistors (FETs) with directly aminated channel surface

Analytica Chimica Acta ◽

10.1016/j.aca.2006.04.051 ◽

2006 ◽

Vol 573-574 ◽

pp. 3-8 ◽

Cited By ~ 33

Author(s):

Kwang-Soup Song ◽

Yusuke Nakamura ◽

Yuichi Sasaki ◽

Munenori Degawa ◽

Jung-Hoon Yang ◽

...

Keyword(s):

Field Effect ◽

Field Effect Transistors ◽

Ph Sensitive ◽

Channel Surface

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A study of borazon-gate pH-sensitive field effect transistors

Journal of Crystal Growth ◽

10.1016/s0022-0248(87)80022-2 ◽

1987 ◽

Vol 82 (4) ◽

pp. 757-760 ◽

Cited By ~ 3

Author(s):

A. Olszyna ◽

W. Włosiński ◽

D. Sobczyńska ◽

W. Torbicz

Keyword(s):

Field Effect ◽

Field Effect Transistors ◽

Ph Sensitive

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Interfacing Biology with Electronic Devices

Solid State Phenomena ◽

10.4028/www.scientific.net/ssp.108-109.789 ◽

2005 ◽

Vol 108-109 ◽

pp. 789-796 ◽

Cited By ~ 2

Author(s):

Andreas Offenhäusser ◽

Sven Ingebrandt ◽

Dirk Mayer

Keyword(s):

Tissue Culture ◽

Information Processing ◽

Molecular Biology ◽

Field Effect ◽

Field Effect Transistors ◽

Electronic Devices ◽

The Other ◽

Pharmacological Effects ◽

Distributed Information ◽

Microelectronic Devices

Due to a number of advances in molecular biology, cell and tissue culture in combination with more sensitive methods to transduce biological signals, it has become increasingly feasible to detect unknown toxicity or pharmacological effects by using biological systems which are electrically coupled to micro- or nanoelectrodes or field-effect transistors (FETs). The coupling of biomolecules with electronic devices is demonstrated. In order to identify the contributions of the various cell signals we have investigated the coupling of cardiac myocytes with FETs. On the other side such systems can also be used to study the very basics of distributed information processing by interfacing cultured neuronal networks with microelectronic devices.

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A microsensor for adenosine-5′-triphosphate pH-sensitive field effect transistors

Analytica Chimica Acta ◽

10.1016/s0003-2670(00)82920-7 ◽

1986 ◽

Vol 187 ◽

pp. 287-291 ◽

Cited By ~ 54

Author(s):

Masao Gotoh ◽

Eiichi Tamiya ◽

Isao Karube ◽

Yasuo Kagawa

Keyword(s):

Field Effect ◽

Field Effect Transistors ◽

Ph Sensitive

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Photocurable pH-sensitive membrane for ion-selective field effect transistors

Talanta ◽

10.1016/j.talanta.2009.11.059 ◽

2010 ◽

Vol 81 (1-2) ◽

pp. 208-212 ◽

Cited By ~ 8

Author(s):

Natalia Abramova ◽

Andrey Bratov

Keyword(s):

Field Effect ◽

Field Effect Transistors ◽

Ph Sensitive ◽

Sensitive Membrane

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Determination of content of indole alkaloids in cell biomass of Rauwolfia serpentina Benth. ex Kurz tissue culture

Current issues in pharmacy and medicine science and practice ◽

10.14739/2409-2932.2021.1.226810 ◽

2021 ◽

Vol 14 (1) ◽

pp. 73-78

Author(s):

O. A. Bieda ◽

I. I. Konvaliuk ◽

L. P. Mozhylevska ◽

S. S. Lukashov ◽

V. A. Kunakh ◽

...

Keyword(s):

Tissue Culture ◽

Indole Alkaloids ◽

Natural Plant ◽

Rauwolfia Serpentina ◽

Cell Biomass ◽

Total Alkaloids ◽

Dry Cell

Cardiovascular diseases are the most common human diseases, hence, the production of cardiological (in particular, anti-arrhythmic) medications from the natural sources is an ever-actual task. Rauwolfia serpentina Benth. is a tropical fruticose plant that is able to produce and concentrate indole alkaloids, especially ajmaline and its derivatives, which are the most effective medications against ventricular arrhythmia with low side effects. Aim of the study. Determination of the qualitative and quantitative content of indole alkaloids in cell biomass of Rauwolfia serpentina tissue culture, obtained by the prolonged in vitro growth. Materials and methods. Object: cell biomass of Rauwolfia serpentina tissue culture (K-27 strain), obtained by methods of long-term cell selection in vitro. Alkaloids content determination: TSQ Vantage LC-MS (ThermoFischer Scientific). Results. 20 indole alkaloids are found in cell biomass of Rauwolfia serpentina tissue culture (K-27 strain). The highest content is registered for ajmaline and its derivatives (0.690 % mass. for ajmaline). The contents of reserpine and yohimbine were found to be as low as 0.009 % and 0.020 %, respectively. Conclusions. It is established that the content of indole alkaloids is higher in K-27 strain in comparison to natural plant and is stable over more than 30 years of its growth. Total alkaloids content was found to be 2.8 % of dry cell biomass, and total ajmaline-type alkaloids content (including ajmaline) was found to be 1.6 % of dry cell biomass. In contrast, the total alkaloid contents in the natural plant material is reported to be in the range of 0.8–1.3 %.

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