Parameters of obtaining tincture from underground organs of Sanguisorba officinalisand study its biological activity

The aim of the work is to experimentally determine the optimal parameters for obtaining a tincture of rhizomes with roots of Sanguisorba officinalis and to investigate its antimicrobial and antioxidant activity. Materials and methods. Rhizomes with roots of Sanguisorba officinalis were harvested in autumn 2019. When establishing the optimal parameters of the technology of obtaining tincture, the evaluation criterion was the number of the sum of polyphenols and the amount of hydroxycinnamic acids, which were determined by spectrophotometric method according to the methods of the State Pharmacopoeia of Ukraine 2.0. Antimicrobial activity was studied by agar diffusion and serial dilutions, antioxidant – in vitro using a stable radical 2,2-diphenyl-1-picrylhydrazyl (2,2-diphenyl-1-picrylhydrazyl – DPPH). Results. The optimal parameters for obtaining a tincture of rhizomes with roots of Sanguisorba officinalis was extraction method maceration at room temperature, raw material-finished product ratio 1:5, time 48 hours, extractant 50 % ethyl alcohol, the number of polyphenols, and the number of hydroxycinnamic acids in terms of dry raw materials not less than 4.0 % and 1.5 % respectively. The resulting tincture exhibits antimicrobial activity against microorganisms Bacillus subtilis ATCC 6633 (growth retardation 26.40 ± 1.04 mm), Staphylococcus aureus ATCC 25923 (growth retardation 24.60 ± 0.68 mm) and Pseudomonas aeruginosa ATCС 27853 (growth retardation 23.60 ± 0.68 mm) and at a dose of 0.02 ml showed antioxidant activity at the level of 70 %. Conclusions. The parameters of obtaining a tincture of rhizomes with roots of Sanguisorba officinalis were determined and its antimicrobial and antioxidant activity was studied.

The experience, results, and possible perspectives of organizing the optimally accessible primary medical care for the rural population of Zaporizhzhia region

Nowadays, the assessment of the activity of the domestic healthcare system is an important and actual issue, especially against the background the active reformation of this industry. One of the important elements of the assessment is studying the availability of primary healthcare to the rural population. The aim of this work is to study the territorial accessibility of primary healthcare, the adequacy and effectiveness of the principles of forming a network of primary care facilities in the rural areas of Zaporizhzhia region that enables to address issues and optimize the location of primary care facilities in the rural areas. Materials and methods. The materials of the research were the data of the official statistical reports for the past 20 years, which characterize the state of health and the degree of medical care accessibility to the rural population. During the research, the technique of complex social and hygienic research was applied, with the use of historical, sociological, sanitary and statistical methods, organizational experiment and others. Results. The article presents the main results of studying the current state of primary healthcare organization for the rural population of Zaporіzhzhia region against the background of active reforming processes. According to the research results, the main elements forming the system of accessibility are territorial, medical, social and economic. The main factors of impact on territorial accessibility are identified. These are: the nature of the settlement of rural residents (density, compactness, service-area radius, the proportion of the rural population, the distance between villages, the distance from a household to a healthcare facility); quality of roads; transport connections between settlements and healthcare facilities; availability of communication means. Conclusions. As a result of the research, the following conclusions were drawn. Such factors as population density, compactness of its location, service-area radius, distance between villages, distance from a household to a healthcare facility, condition and quality of roads, transport connections between settlements and healthcare facilities are important during forming or improving the network of healthcare facilities in rural areas. These factors must be considered for the rational placement of primary care facilities in rural areas.

Means of occupational therapy in (individual) rehabilitation programs for people with Parkinson’s disease

The aim of the work is to develop a set of rehabilitation measures including occupational therapy classes for Parkinson’s disease and to assess the objective condition of patients on the scales of non-motor symptoms, cognitive and mental disorders. Materials and methods. The study of patients was conducted on the basis of anamnesis, objective examination and scales for assessment of non-motor symptoms, cognitive and mental disorders (scale of non-motor symptoms – NMSS, scale of mental disorders – MMSE, Montreal scale of cognitive disorders – MOCA); to assess non-motor symptoms, a patient questionnaire was additionally used to identify autonomic disorders (according to A. M. Wayne, 1998). The control and experimental groups were offered exercise, physiotherapy, massage and nutrition (diet). Occupational therapy was added for the experimental group. Results. After the physical rehabilitation, the condition of the patients significantly improved. The final score in the experimental group of patients with non-motor manifestations of Parkinson’s disease on the scale of assessment of non-motor symptoms was statistically significantly lower and was 61.04 ± 0.39 (P < 0.01). The final score on the scale of mental disorders was statistically significantly higher – 27.78 ± 0.27 (P < 0.01), on the scale of cognitive changes, it also became statistically significantly higher – 25.13 ± 0.19 (P < 0.01). According to A. M. Wayne’s questionnaire of autonomic disorders, the total score in the control group was 64.0 ± 3.2. In the experimental group of patients, the total score was 51.0 ± 5.4 points (P < 0.01). Thus, under the influence of additional occupational therapy classes, in the experimental group both on the scale of non-motor symptoms and by A. M. Wayne’s, questionnaire there was a statistically significant improvement in the condition of patients. Conclusions. Based on the results of the study, it can be concluded that there is a significant difference (P < 0.01) in the condition of patients on the final scores, with the pronounced improvement in the condition of the experimental group patients in comparison with the control group patients, following the results of all survey scales. A significant difference is observed on the scale of non-motor symptoms. A certain role in improving the condition of patients with appropriate screening using study scales and a patient questionnaire to identify autonomic disorders (according to A. M. Wayne, 1998), apparently played a component of the rehabilitation program, which further included occupational therapy classes.

Formation of the demand for contraceptives using the Van Westendorp method based on establishing consumer value

One of the main indicators of the consumer value of a product is the price. Its controversial idealistic and materialistic basis is aimed at securing the interests of two opposites: producer-seller and buyer. Correctly made pricing decisions, taking into account the requirements of both players in the pharmaceutical market, will effectively regulate the processes of the pharmaceutical business and the supply system. The aim: approbation of the Van Westendorp method on the example of contraceptives; establishing their consumer value for further rational decision-making regarding the improvement of the system for providing contraceptives to Ukrainian female consumers. Materials and methods. The research used the marketing Van Westendorp method based on surveying a quota sample of women of reproductive age from one of the Ukrainian regions. The main calculations were performed with MS Excel 2010 software. Results. The survey of 420 women enabled to set the maximum range of readiness to pay (the point of maximum cheapness and high cost) for the most advanced pharmaceutical types of contraception (hormonal, non-hormonal and medical products) in the age and income quotas. It was established that the price sensitivity depends on social and economic indicators. Conclusions. The established maximal ranges of willingness to pay are well below market prices for contraceptives. The exception is cheap and widespread contraception (aerosol and male condom), which confirms the need to use marketing research methods to meet the needs of the female consumer.

Synthesis and properties of S-derivatives of 4-amino-5-(5-methylpyrazol-3-yl)-1,2,4-triazole-3-thiol

The combination of various heterocyclic systems with a wide range of properties is quite expedient and is, in practice, a justified direction for obtaining biologically active substances, which ultimately forms a favorable basis for the creation of drugs. In recent decades, the attention of scientists has been closely focused on nitrogen-containing heterocyclic compounds. Among such compounds, 1,2,4-triazole and pyrazole occupy a special place. Indeed, on the basis of these systems, a significant number of well-known drugs have been created, which are widely used at the present time. The aim of the work was the synthesis of S-derivatives of 4-amino-5-(5-methylpyrazol-3-yl)-1,2,4-triazole-3-thiol, study of their physical and chemical properties, pre-screening studies with subsequent establishment of the feasibility of further pharmacological studies. Materials and methods. Experimental methods of organic chemistry: synthesis using microwave activation, physical and chemical methods for the analysis of organic compounds (determination of the melting point, elemental analysis, 1H NMR, IR spectroscopy and chromatography-mass spectrometry). Methods for in silico pre-screening studies to establish the biological potential in several synthesized compounds (molecular docking). Results. 10 new S-derivatives of 4-amino-5-(5-methylpyrazol-3-yl)-1,2,4-triazole-3-thiol were synthesized. The structure of the obtained compounds was confirmed by a set of physical and chemical methods of analysis. According to the results of prescreening studies, the main directions of research of biological properties of synthesized compounds were provided. Conclusions. The expediency of using microwave irradiation in the synthesis of a series of S-alkyl derivatives of 4-amino-5-(5-methylpyrazol-3-yl)-1,2,4-triazole-3-thiol had been proved. Based on the results of in silico studies, the expediency of further studies of anti-inflammatory, antifungal and anticancer activities in several synthesized compounds had been substantiated.

Pathomorphological changes of capillaries in the cerebral cortex in type 2 diabetes mellitus

The aim of the study was to determine microscopic, immunohistochemical, electron microscopic, morphometric parameters of capillaries in the cerebral cortex in deceased patients with type 2 diabetes mellitus (DM) in comparison with the conditional control group and the group of deceased patients with dyscirculatory ischemic encephalopathy. Materials and methods. Microscopic, immunohistochemical, electron microscopic and morphometric studies of the cerebral cortex capillaries were performed in 3 groups: group I – 20 deceased patients with type 2 DM, group II conditional control (CC) – 20 deceased patients without clinical morphological signs of DM and cerebrovascular pathology, group III – 20 patients with dyscirculatory ischemic encephalopathy (DIEP). Results. It was found that in type 2 DM, the capillaries in the cerebral cortex lose pericytes due to their apoptosis: the number of pericytes in the cortical capillaries in type 2 DM was statistically significantly lower by 57.14 % compared to CC group and 50.00 % lower compared to DIEP group, the level of caspase-3 expression in the cortical microvessels in type 2 DM was significantly higher by 230.85 % compared with CC group and 81.67 % higher than in DIEP group. According to electron microscopy in type 2 DM, apoptosis of pericytes and single endothelial cells in the cerebral cortex capillaries was determined as well as significant expansion of basement membranes with the accumulation of electron-dense amorphous material and collagen fibrils. According to the results of morphometry, the outer diameter of the cortical capillaries in type 2 DM group was 4.90 % significantly larger, the inner diameter was 9.78 % smaller and the walls were 66.62 % thicker (compared with CC group) due to the accumulation of PAS-positive substances of blood serum and fibrosis, confirmed by 22.96 % greater area of type IV collagen expression in the microvessel walls. Conclusions. The pathomorphological changes of microvessels identified in deceased patients with type 2 diabetes mellitus are signs of diabetic cerebral microangiopathy.

Dynamics of general physical fitness indicators in the system of physical therapy of 11–13-year-old women weightlifters after traumatic injuries of the musculoskeletal system

The aim of the work to determine the dynamic characteristics of overall physical condition indicators in 11–13-year-old female weightlifters after injuries of the musculoskeletal system at the training stage of physical therapy. Materials and methods. The experimental population comprised 45 female weightlifters (aged 11–13 years, qualification ‒ I, II, III junior categories) from SСYSSOR “Spartak” of the Zaporizhzhia regional council, CYSS “Kolos” of Kamiansko-Dniprovskyi district of Zaporizhzhia region, Berdiansk CYSS of Zaporizhzhia Region City Council, and students of Kharkiv Regional Higher School of Physical Culture and Sports majoring in weightlifting. Patients after musculoskeletal injuries were divided into two groups: main (MG, n = 22) and control (CG, n = 23). Patients of the control group underwent standard treatment (generally prescribed in medical establishments). For main group patients in the training period, the program of physical therapy has been developed and implemented, with the current control of overall physical condition indicators. Research methods. Analysis of literature and sources of information presented on the Internet; clinical observations; pedagogical testing; methods of mathematical statistics. Results. Analysis of overall physical condition indicators of athletes of 11–13-year-old weightlifters of the main and control groups during the recovery and training periods allows us to state that main group athletes have higher growth rates, and dynamic characteristics of their overall physical condition are linear with constant focus on improvement. According to the indicators of “running 30 m, s” test, the following growth indicators were recorded: main group athletes (-0.48), control group athletes (-0.29), the difference (-0.19). According to the indicators of “standing long jump, cm” test, the following growth indicators were recorded: main group athletes (+14), control group athletes (+5), difference (+9). According to the results of the test “flexion-extension of the arms in the supine position, n” the following indicators were obtained: main group athletes (+4.44), control group athletes (+2.86), difference (+1.58). Conclusions. Intensification of the training process and forcing the training of junior athletes in weightlifting are the leading factors that result in injuries of the musculoskeletal system, multisystem pathologies and the development of pathomorphic phenomena. Indicators of overall physical condition of weightlifters aged 11–13 allow us to state that main group athletes have a higher difference in growth, and the tendency of their overall physical condition has a linear focus on improvement. Indicators of overall physical condition of control group athletes are nonlinear, according to “running 30 m, s” and “standing long jump, cm” the test results.

Medical and geographical determinants of oncopathology in the population

The aim: to study the permanent risk factor of oncopathology in order to determine its medical and geographical features and assess their impact on oncopathology of the population of Zaporizhzhia region. Materials and methods. By means of spatial and geographical, as well as analytical and statistical methods, territories were medically and statistically differentiated, with the assessment of the natural environment quality. Epidemiological study of malignant neoplasms was conducted retrospectively for the period of 2004–2015, in accordance with the International Classification of Diseases ICD-10. Determination of environmental impact on oncopathology was performed using the attributive fraction. Results. Geoactive space is characterized by a variety of natural and climatic conditions of lithosphere, hydrosphere and landscapes. The main physical and geographical objects of the Zaporizhzhia region (Ukrainian Crystal Shield, the Dnipro, the Sea of Azov) form six zones, which differ in the diversity of system-forming elements. Specific features of discontinuous disturbances of the lithosphere of the Ukrainian crystal shield are abnormally high electrical conductivity of the earth’s crust, intense electromagnetic fields, high concentration of radon in aquifers, and streams of high-energy fluids. Conclusions. The highest geopathogenic impact is manifested in the fault zone of the Ukrainian crystal shield. The highest values of the attributive fraction of malignant neoplasms were registered in this zone among the whole population (26.8 %), among the adult population (25.4 %) and the able-bodied population (32.7 %). The oncopathological potential of natural conditions was least manifested in the valley of the Dnipro breakthrough and the Azov Upland, where the attributive fraction for almost all indicators did not exceed 8.2 %.

Research of the chemical composition of Artemisia tschernieviana Bess. by gas chromatography method with mass detection

The aim of the work was to study chromato-mass spectroscopic of Artemisia tschernieviana Bess. grass and to establish possible prospects for the use of this plant in medical practice. Materials and methods. Artemisia tschernieviana Bess. grass was chosen as the object of research. Raw materials were collected in July 2021 in the flowering phase (Zaporizhzhia). The tincture was obtained by maceration and the raw material was extracted with methyl alcohol at room temperature for 10 days according to the method of making tinctures. The study of the chemical composition of Artemisia tschernieviana Bess. Grass was carried out using gas chromatograph Agilent 7890B GC System (Agilent, Santa Clara, CA, USA) with mass spectrometric detector Agilent 5977 BGC/MSD (Agilent, Santa Clara, CA, USA) and chromatographic column DB-5ms (30 m × 250 μm × 0.25 μm). Results. During the study it was found that the composition of Artemisia tschernieviana Bess. grass includes 21 compounds (2 in the isomeric state). β-myrcene (17.22 %), sabinene (14.54 %), 2-(4a,8-dimethyl-7-oxo-1,2,3,4,4a,7-hexahydronaphthalen-2-yl) propionic acid (11.57 %), 2-dodecen-1-yl-succinic anhydride (10.84 %) and chamazulene (9.02 %) accounted for the largest percentage. According to the literature review, myrcene has antibacterial, anesthetic and analgesic effects. Sabinene is characterized by antibacterial action. 2-(4a,8-dimethyl-7-oxo-1,2,3,4,4a,7-hexahydronaphthalen-2-yl)-propionic acid is a fatty acid with possible antibacterial and anti-inflammatory activity. 2-dodecen-1-yl-succinic anhydride is used to produce anhydride-modified form of insulin that can be used in the treatment of diabetes. Hamazulen has anti-inflammatory activity. Conclusions. In view of all the above, we can conclude that Artemisia tschernieviana Bess. grass can be recommended for further research as a source of potential anti-inflammatory and antibacterial agents.

The microscopic diagnostic signs of some Fabaceae L. genus representatives. Message ІІ. Plant conducting system

The world plant products market is expanded rapidly and trade in them tends to grow by 15–25 % annually. The number of reports is increased proportionally about accidental contamination or deliberate, economically motivated falsification of plant raw materials. 27 % of the nearly 6.000 herbal preparations that are sold in 37 countries have contained undeclared contaminants, substitutes, or other components, according to the literature. We have conducted a plant conduction system microscopic analysis of the individual members’ genus Clover (Trifoliae L.), Fabaceae L. to identify morphoanatomical characteristics. Clover has anti-inflammatory, antiseptic, choleretic, diaphoretic, diuretic, hemostatic, expectorant, astringent properties and is used in many diseases. Aim. The finding common features and those that differ and can be used as diagnostic during studying the conducting system structure of genus Trifolium L. leaves and stems. Materials and methods. Plant material (herb) from Trifolium pratense L., T. incarnatum L., T. repens L. and T. fragiferum L. was harvested during the active flowering period – (May – June) and was dried in a well-ventilated place. Leaves and stems preparations were pre-boiled in 5 % sodium hydroxide water solution and fixed in chloral hydrate solution. Cross-sections were made with a microtome. The BIOLAM LOMO light microscope (Russia) and OLYMPUS SH-21 digital camera were used to record the data about identify the conducting apparatus of the plant’s leaf, petiole, and stem. Results. It has been examined the central vein structure of T. pratense L. and T. fragiferum L. leaves, it was determined that the conductive system is covered with a crystalline coating and there is one closed collateral bundle in the center, which is not typical for dicotyledonous plants. The petioles of T. incarnatum L., T. fragiferum L., and T. repens L. in cross-section are several different shapes. There are kidney-shaped and round. The conducting apparatus T. incarnatum L. and T. repens L. have arranged in a circle, closed and collateral. The leafstalk structure type of T. fragiferum L. is bunchles. It contradicts too the information about the structure conducting system of dicotyledonous plants. The stem’s conducting bundles are collateral and open. Conclusions. We have paid attention to the structural peculiarities of the conductive system of the central vein and petiole of objects for study: Trifolium pratense L., T. incarnatum L., T. repens L., and T. fragiferum L. when searching for differentiating features in some members of the genus Trifoliae L. in pharmacognostic analysis. The Dicotyledonae representatives are not characteristic of the closed type of conductive bundles, which we observed during microscopic examination.