scholarly journals DEVELOPMENT OF AN ENZYME BIOSENSOR BASED ON pH-SENSITIVE FIELD-EFFECT TRANSISTORS FOR ESTIMATING THE TOTAL CONTENT OF INDOLE ALKALOIDS IN TISSUE CULTURE OF RAUWOLFIA SERPENTINA BENTH. EX KURZ

2021 ◽  
Vol 18 (3) ◽  
pp. 38-50
Author(s):  
V. M. Arkhypova ◽  
О. О. Soldatkin ◽  
L. P. Moghylevska ◽  
І. І. Konvalyuk ◽  
V. А. Kunakh ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Field Effect ◽  
Field Effect Transistors ◽  
Indole Alkaloids ◽  
Total Content ◽  
High Sensitivity ◽  
Ph Sensitive ◽  
Biologically Active ◽  
Rauwolfia Serpentina ◽  
Enzyme Biosensor

A laboratory prototype of an enzyme biosensor based on pH‑sensitive field-effect transistors has been developed to determine the total content of indole alkaloids in Rauwolfia serpentina Benth. Ex Kurz tissue culture. The biosensor was characterized by high sensitivity to th A laboratory prototype of an enzyme biosensor based on pH‑sensitive field effect transistors has been developed to determine the total content of indole alkaloids in Rauwolfia serpentina Benth. Ex Kurz tissue culture. The biosensor was characterized by high sensitivity to the total content of indole alkaloids (minimum limit of determination – 0.5 μg/ml of the total content of indole alkaloids contained in the juice obtained from tissue culture of Rauwolfia serpentina). The linear range of biosensor determination of the analyte was from 2 to 15 μg / ml of the total content of indole alkaloids. Analysis of indole alkaloids using a biosensor is simple and fast and does not require expensive equipment and special sample preparation for analysis, unlike traditional methods. The created biosensor can be further used to control the total content of indole alkaloids in modern biotechnological and pharmaceutical processes for the production of drugs and biologically active additives. e total content of indole alkaloids (minimum limit of determination – 0.5 μg/ml of the total content of indole alkaloids contained in the juice obtained from tissue culture of Rauwolfia serpentina). The linear range of biosensor determination of the analyte was from 2 to 15 μg / ml of the total content of indole alkaloids. Analysis of indole alkaloids using a biosensor is simple and fast and does not require expensive equipment and special sample preparation for analysis, unlike traditional methods. The created biosensor can be further used to control the total content of indole alkaloids in modern biotechnological and pharmaceutical processes for the production of drugs and biologically active additives.  

A Novel Enzyme Biosensor Specific for Formaldehyde Based on pH-Sensitive Field Effect Transistors

1997 ◽  
Vol 68 (2) ◽  
pp. 209-213 ◽  
Author(s):  
Yaroslav I. Korpan ◽  
Alexey P. Soldatkin ◽  
Mikhailo V. Gonchar ◽  
Andrii A. Sibirny ◽  
Timothy D. Gibson ◽  
...  
Keyword(s):  
Field Effect ◽  
Field Effect Transistors ◽  
Ph Sensitive ◽  
Enzyme Biosensor

A novel enzyme biosensor for steroidal glycoalkaloids detection based on pH-sensitive field effect transistors

2002 ◽  
Vol 55 (1-2) ◽  
pp. 9-11 ◽  
Author(s):  
Y.I Korpan ◽  
V.V Volotovsky ◽  
C Martelet ◽  
N Jaffrezic-Renault ◽  
E.A Nazarenko ◽  
...  
Keyword(s):  
Field Effect ◽  
Field Effect Transistors ◽  
Ph Sensitive ◽  
Enzyme Biosensor

Development of enzyme biosensor based on pH-sensitive field-effect transistors for detection of phenolic compounds

2002 ◽  
Vol 55 (1-2) ◽  
pp. 79-81 ◽  
Author(s):  
S.V Dzyadevych ◽  
T Mai Anh ◽  
A.P Soldatkin ◽  
N Duc Chien ◽  
N Jaffrezic-Renault ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Field Effect ◽  
Field Effect Transistors ◽  
Ph Sensitive ◽  
Enzyme Biosensor

scholarly journals Capacitive Field-effect (bio-)chemical Sensors Based on Nanocrystalline Diamond Films

2009 ◽  
Vol 1203 ◽  
Author(s):  
Matthias Bäcker ◽  
Arshak Poghossian ◽  
Maryam H. Abouzar ◽  
Sylvia Wenmackers ◽  
Stoffel D. Janssens ◽  
...  
Keyword(s):  
Thin Films ◽  
Field Effect ◽  
High Sensitivity ◽  
Ph Sensitive ◽  
Nanocrystalline Diamond ◽  
Penicillin G ◽  
Layer By Layer ◽  
Label Free ◽  
High Coverage ◽  
Layer Adsorption

AbstractCapacitive field-effect electrolyte-diamond-insulator-semiconductor (EDIS) structures with O-terminated nanocrystalline diamond (NCD) as sensitive gate material have been realized and investigated for the detection of pH, penicillin concentration, and layer-by-layer adsorption of polyelectrolytes. The surface oxidizing procedure of NCD thin films as well as the seeding and NCD growth process on a Si-SiO2 substrate have been improved to provide high pH-sensitive, non-porous thin films without damage of the underlying SiO2 layer and with a high coverage of O-terminated sites. The NCD surface topography, roughness, and coverage of the surface groups have been characterized by SEM, AFM and XPS methods. The EDIS sensors with O-terminated NCD film treated in oxidizing boiling mixture for 45 min show a pH sensitivity of about 50 mV/pH. The pH-sensitive properties of the NCD have been used to develop an EDIS-based penicillin biosensor with high sensitivity (65-70 mV/decade in the concentration range of 0.25-2.5 mM penicillin G) and low detection limit (5 μM). The results of label-free electrical detection of layer-by-layer adsorption of charged polyelectrolytes are presented, too.

pH-sensitive diamond field-effect transistors (FETs) with directly aminated channel surface

2006 ◽  
Vol 573-574 ◽  
pp. 3-8 ◽  
Author(s):  
Kwang-Soup Song ◽  
Yusuke Nakamura ◽  
Yuichi Sasaki ◽  
Munenori Degawa ◽  
Jung-Hoon Yang ◽  
...  
Keyword(s):  
Field Effect ◽  
Field Effect Transistors ◽  
Ph Sensitive ◽  
Channel Surface

A study of borazon-gate pH-sensitive field effect transistors

1987 ◽  
Vol 82 (4) ◽  
pp. 757-760 ◽  
Author(s):  
A. Olszyna ◽  
W. Włosiński ◽  
D. Sobczyńska ◽  
W. Torbicz
Keyword(s):  
Field Effect ◽  
Field Effect Transistors ◽  
Ph Sensitive

Interfacing Biology with Electronic Devices

2005 ◽  
Vol 108-109 ◽  
pp. 789-796 ◽  
Author(s):  
Andreas Offenhäusser ◽  
Sven Ingebrandt ◽  
Dirk Mayer
Keyword(s):  
Tissue Culture ◽  
Information Processing ◽  
Molecular Biology ◽  
Field Effect ◽  
Field Effect Transistors ◽  
Electronic Devices ◽  
The Other ◽  
Pharmacological Effects ◽  
Distributed Information ◽  
Microelectronic Devices

Due to a number of advances in molecular biology, cell and tissue culture in combination with more sensitive methods to transduce biological signals, it has become increasingly feasible to detect unknown toxicity or pharmacological effects by using biological systems which are electrically coupled to micro- or nanoelectrodes or field-effect transistors (FETs). The coupling of biomolecules with electronic devices is demonstrated. In order to identify the contributions of the various cell signals we have investigated the coupling of cardiac myocytes with FETs. On the other side such systems can also be used to study the very basics of distributed information processing by interfacing cultured neuronal networks with microelectronic devices.

scholarly journals Advances in the fabrication of graphene transistors on flexible substrates

2017 ◽  
Vol 8 ◽  
pp. 467-474 ◽  
Author(s):  
Gabriele Fisichella ◽  
Stella Lo Verso ◽  
Silvestra Di Marco ◽  
Vincenzo Vinciguerra ◽  
Emanuela Schilirò ◽  
...  
Keyword(s):  
Field Effect ◽  
Field Effect Transistors ◽  
High Mobility ◽  
High Sensitivity ◽  
Atomic Layer ◽  
Transparent Electrode ◽  
Large Area ◽  
Sensing Applications ◽  
Dielectric Performance

Graphene is an ideal candidate for next generation applications as a transparent electrode for electronics on plastic due to its flexibility and the conservation of electrical properties upon deformation. More importantly, its field-effect tunable carrier density, high mobility and saturation velocity make it an appealing choice as a channel material for field-effect transistors (FETs) for several potential applications. As an example, properly designed and scaled graphene FETs (Gr-FETs) can be used for flexible high frequency (RF) electronics or for high sensitivity chemical sensors. Miniaturized and flexible Gr-FET sensors would be highly advantageous for current sensors technology for in vivo and in situ applications. In this paper, we report a wafer-scale processing strategy to fabricate arrays of back-gated Gr-FETs on poly(ethylene naphthalate) (PEN) substrates. These devices present a large-area graphene channel fully exposed to the external environment, in order to be suitable for sensing applications, and the channel conductivity is efficiently modulated by a buried gate contact under a thin Al2O3 insulating film. In order to be compatible with the use of the PEN substrate, optimized deposition conditions of the Al2O3 film by plasma-enhanced atomic layer deposition (PE-ALD) at a low temperature (100 °C) have been developed without any relevant degradation of the final dielectric performance.

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