Radiation-induced DNA damage and repair in peripheral blood mononuclear cells from Nijmegen breakage syndrome patients and carriers assessed by the Comet assay

2006 ◽  
Vol 47 (4) ◽  
pp. 260-270 ◽  
Author(s):  
Susann Bürger ◽  
Detlev Schindler ◽  
Martin Fehn ◽  
Bettina Mühl ◽  
Hartmut Mahrhofer ◽  
...  
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Nijmegen Breakage Syndrome ◽  
Peripheral Blood Mononuclear ◽  
Dna Damage And Repair ◽  
Blood Mononuclear Cells ◽  
Radiation Induced

scholarly journals Dose-related genotoxic effect of T-2 toxin measured by comet assay using peripheral blood mononuclear cells of healthy pigs

2013 ◽  
Vol 61 (2) ◽  
pp. 175-186 ◽  
Author(s):  
Katalin Horvatovich ◽  
Dóra Hafner ◽  
Zsófia Bodnár ◽  
Gergely Berta ◽  
Csaba Hancz ◽  
...  
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Trichothecene Mycotoxin ◽  
Peripheral Blood Mononuclear ◽  
Dna And Rna ◽  
Blood Mononuclear Cells ◽  
Dna And Rna Synthesis

T-2 toxin is the most acutely toxic trichothecene mycotoxin: it inhibits protein, DNA and RNA synthesis. The main goal of this study was to evaluate the rate of DNA damage caused by T-2 toxin in porcine mononuclear cells in increasing concentrations (0.1, 0.5 and 1.0 μmol) and after two different incubation periods (24 and 42 h). The lowest concentration caused DNA damage and about 50% of the treated cells could be categorised as having 1 to 4 scores in comet assay. In parallel with the increase of T-2 toxin concentration, the frequency of intact lymphocytes decreased from 50.2% (0.1 μM) to 36.3% (1.0 μM) in the first 24 h. In case of score 3, the highest concentration of T-2 toxin resulted in a 5-fold change, as compared to the lowest dose. Cells with score 4 were found only after exposure to 1.0 μM T-2 toxin. The exposure time did not have a significant effect on the results, while concentration did (P < 0.0001). However, a significant interaction between concentration and time as fixed factors (P < 0.0001) was found. When these were combined as a single factor, the results showed a significant toxin treatment effect on the results. It was concluded that a time- and dose-dependent DNA damaging effect of T-2 toxin could be demonstrated using peripheral blood mononuclear cells from healthy pigs by comet assay.

scholarly journals Application of the Comet Assay for Investigation of Oxidative DNA Damage in Equine Peripheral Blood Mononuclear Cells

2004 ◽  
Vol 134 (8) ◽  
pp. 2133S-2140S ◽  
Author(s):  
David J. Marlin ◽  
Lucy Johnson ◽  
Demelza A. Kingston ◽  
Nicola C. Smith ◽  
Chris M. Deaton ◽  
...  
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Oxidative Dna Damage ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

scholarly journals α-Particle-induced DNA damage tracks in peripheral blood mononuclear cells of [223Ra]RaCl2-treated prostate cancer patients

2021 ◽  
Author(s):  
S. Schumann ◽  
U. Eberlein ◽  
C. Lapa ◽  
J. Müller ◽  
S. Serfling ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Dna Damage ◽  
Cancer Patients ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Absorbed Dose ◽  
Peripheral Blood Mononuclear ◽  
Absorbed Doses ◽  
Blood Mononuclear Cells

Abstract Purpose One therapy option for prostate cancer patients with bone metastases is the use of [223Ra]RaCl2. The α-emitter 223Ra creates DNA damage tracks along α-particle trajectories (α-tracks) in exposed cells that can be revealed by immunofluorescent staining of γ-H2AX+53BP1 DNA double-strand break markers. We investigated the time- and absorbed dose-dependency of the number of α-tracks in peripheral blood mononuclear cells (PBMCs) of patients undergoing their first therapy with [223Ra]RaCl2. Methods Multiple blood samples from nine prostate cancer patients were collected before and after administration of [223Ra]RaCl2, up to 4 weeks after treatment. γ-H2AX- and 53BP1-positive α-tracks were microscopically quantified in isolated and immuno-stained PBMCs. Results The absorbed doses to the blood were less than 6 mGy up to 4 h after administration and maximally 16 mGy in total. Up to 4 h after administration, the α-track frequency was significantly increased relative to baseline and correlated with the absorbed dose to the blood in the dose range < 3 mGy. In most of the late samples (24 h – 4 weeks after administration), the α-track frequency remained elevated. Conclusion The γ-H2AX+53BP1 assay is a potent method for detection of α-particle-induced DNA damages during treatment with or after accidental incorporation of radionuclides even at low absorbed doses. It may serve as a biomarker discriminating α- from β-emitters based on damage geometry.

DNA damage in peripheral blood mononuclear cells of patients undergoing anti-tuberculosis treatment

2012 ◽  
Vol 747 (1) ◽  
pp. 82-85 ◽  
Author(s):  
Larissa Ragozo Cardoso de Oliveira ◽  
Eliana Peresi ◽  
Francilene Capel Tavares ◽  
Camila Renata Corrêa ◽  
Damiana Tortolero Pierine ◽  
...  
Keyword(s):  
Dna Damage ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Tuberculosis Treatment ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

scholarly journals Kaempferol derivatives isolated from Lens culinaris Medik. reduce DNA damage induced by etoposide in peripheral blood mononuclear cells

2019 ◽  
Vol 8 (6) ◽  
pp. 896-907 ◽  
Author(s):  
Magdalena Kluska ◽  
Michał Juszczak ◽  
Daniel Wysokiński ◽  
Jerzy Żuchowski ◽  
Anna Stochmal ◽  
...  
Keyword(s):  
Dna Damage ◽  
Cell Viability ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Lens Culinaris ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Aerial Parts ◽  
Kaempferol Glycoside ◽  
Blood Mononuclear Cells

Abstract Bioactive compounds isolated from plants are considered to be attractive candidates for cancer therapy. In this study, we examined the effect of kaempferol, its derivatives, the polyphenol fraction (PF) and an extract (EX) isolated from the aerial parts of Lens culinaris Medik. on DNA damage induced by etoposide in human cells. We also studied the effect of these compounds and their combinations on cell viability. The studies were conducted on HL-60 cells and human peripheral blood mononuclear cells (PBMCs). We used the comet assay in the alkaline version to evaluate DNA damage. To examine cell viability we applied the trypan blue exclusion assay. We demonstrated that kaempferol glycoside derivatives isolated from the aerial parts of Lens culinaris Medik. reduce DNA damage induced by etoposide in PBMCs, but do not have an impact on DNA damage in HL-60 cells. We also showed that kaempferol induces DNA damage in HL-60 cells and leads to an increase of DNA damage provoked by etoposide. Our data suggest that kaempferol derivatives can be further explored as a potential agent protecting normal cells against DNA damage induced by etoposide. Moreover, kaempferol's ability to induce DNA damage in cancer cells and to increase DNA damage caused by etoposide may be useful in designing and improving anticancer therapies.

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