Multigenerational effects of nickel on Daphnia magna
 depend on temperature and the magnitude of the effect in the first generation

AbstractBackgroundChronic antibiotic exposure impacts host health through changes to the microbiome, increasing disease risk and reducing the functional repertoire of community members. The detrimental effects of antibiotic perturbation on microbiome structure and function after one host generation of exposure have been well-studied. However, much less is understood about the multigenerational effects of antibiotic exposure and how the microbiome may recover across host generations.ResultsIn this study, we examined microbiome composition and host fitness across five generations of exposure to a suite of three antibiotics in the model zooplankton host Daphnia magna. By utilizing a split-brood design where half of the offspring from antibiotic-exposed parents were allowed to recover and half were maintained in antibiotics, we aimed to examine recovery and resilience of the microbiome. Unexpectedly, we discovered that experimental isolation of single host individuals across generations also exerted a strong effect on microbiome composition, with composition becoming less diverse over generations regardless of treatment. Simultaneously, Daphnia magna body size and cumulative reproduction increased across generations while survival decreased. Though antibiotics did cause substantial changes to microbiome composition, the microbiome generally became similar to the no antibiotic control treatment within one generation of recovery no matter how many prior generations were spent in antibiotics.ConclusionsContrary to results found in vertebrate systems, Daphnia magna microbiome composition recovers quickly after antibiotic exposure. However, our results suggest that the isolation of individual hosts leads to the stochastic extinction of rare taxa in the microbiome, indicating that these taxa are likely maintained via transmission in host populations rather than intrinsic mechanisms. This may explain the intriguing result that microbiome diversity loss increased host fitness.

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Microplastics but not natural particles induce multigenerational effects in Daphnia magna

Environmental Pollution ◽

10.1016/j.envpol.2019.113904 ◽

2020 ◽

Vol 260 ◽

pp. 113904 ◽

Cited By ~ 4

Author(s):

Christoph Schür ◽

Sebastian Zipp ◽

Tobias Thalau ◽

Martin Wagner

Keyword(s):

Daphnia Magna ◽

Multigenerational Effects

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Multigenerational Effects of the Antibiotic Tetracycline on Transcriptional Responses of Daphnia magna and Its Relationship to Higher Levels of Biological Organizations

Environmental Science & Technology ◽

10.1021/acs.est.7b05050 ◽

2017 ◽

Vol 51 (21) ◽

pp. 12898-12907 ◽

Cited By ~ 10

Author(s):

Hyun Young Kim ◽

Jana Asselman ◽

Tae-Yong Jeong ◽

Seungho Yu ◽

Karel A. C. De Schamphelaere ◽

...

Keyword(s):

Daphnia Magna ◽

Transcriptional Responses ◽

Multigenerational Effects

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THE IMPACT OF NON-LOCAL ACTIVATION AND ASSOCIATED WATER PHASE PARA-STATE ON AN AQUATIC ORGANISMS DAPHNIA MAGNA LIFETIME

Hygiene and Sanitation ◽

10.18821/0016-9900-2019-98-11-1251-1256 ◽

2019 ◽

Vol 98 (11) ◽

pp. 1251-1256

Author(s):

T. I. Iksanova ◽

D. B. Kamenetskya ◽

A. A. Stekhin ◽

G. V. Yakovleva ◽

M. G. Kochetkova

Keyword(s):

Life Expectancy ◽

Daphnia Magna ◽

First Generation ◽

Second Generation ◽

Water Phase ◽

2Nd Generation ◽

Water Activation ◽

Non Local ◽

Quantum Coupling ◽

Local Water

Introduction. The work is devoted to the study of associated water phase magnetic state influence, represented by amorphous ice with a modified ratio of isomers in the increasing of para-water proportion, and non-local water activation based on the technology of non-local quantum water conjugation with the production activating process, on the vital hydrobionts Daphnia magna signs. Material and methods. The studies were carried out using non-locally activated drinking water “Si” and a para-water concentrate prepared in hypomagnetic conditions at a residual magnetic flux density not more than 20ntl according to the manufacturer’s technology. The para-water concentrate was introduced into the hydrobionts medium in the ratio of 9 ml per 1 liter of water under study. To obtain offspring, individuals were used, derived on the settled tap water (1st generation) and individuals, derived on the water “Si” - 2nd generation. One-day individuals of Daphnia magna were placed in the amount of 20pcs/l of the studied water and the entire life cycle were kept in optimal conditions: climatostat P-2 (temperature - 20±1°C, illumination - 1200-2500lux, photoperiod - 12/12) hour. Feeding of Daphnia was carried out by Chlorella microalgae grown on the medium “Tamiya”-50% with a density D=0.65-0.72. Results. The life expectancy of Daphnia magna hydrobionts in the non-locally activated environment (water “Si”) increases by an average of 38 days in the first generation and 50 days in the second generation, and in an environment with a potentiating additive para-water associates for 58 days (first generation) and 41 days (second generation).The life expectancy of aquatic animals is related by negative correlation with the change in values of the oxidation-reductant potential (Eh) of the environment of hydrobionts development (Corr.=-0.993 (1st generation). Both non-local water activation and spin-modification of its associated phase by para-isomers demonstrate an increase in the hydrobionts productivity: - in non-locally activated water - 1.20 (1st generation) and 2.68 (2nd generation); - in non-locally activated water with the addition of para-water - 1.39 (1st generation) and 1.97 (2nd generation) times. Conclusion. From the results it follows that the determining factor in the hydrobionts medium is its quantum coupling with electron sources in the external environment. This determines the importance of water quantum coupling with natural ecosystems, affecting cellular metabolism. Spin para modification of the associated water phase leads to an additional increase in the hydrobionts life expectancy in the first generation. In the second generation, the influence of para-water is accompanied by some decrease in both the life hydrobionts expectancy and their productivity.

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Transgenerational Effects of Environmentally Relevant Concentrations of Atrazine and Glyphosate Herbicides, Isolated and in Mixture, to Freshwater Microcrustacean Daphnia Magna

10.21203/rs.3.rs-790734/v1 ◽

2021 ◽

Author(s):

Wanessa A Ramsdorf ◽

Eduarda Roberta Bordin ◽

Renan cesar Munhoz ◽

Paloma Pucholobek Panicio ◽

Adriane Martins Freitas

Keyword(s):

Daphnia Magna ◽

First Generation ◽

Reproductive Capacity ◽

Long Term Effects ◽

Glutathione S Transferase ◽

Biochemical Biomarkers ◽

Two Generations ◽

Test Organisms ◽

Herbicide Mixture ◽

Analytical Standards

Abstract Herbicide mixture is used as an alternative to obtain different mechanisms of action acting on weeds, resulting in the frequent presence of pesticides in environmental compartments. As they are products used worldwide, this study evaluated effects of environmentally relevant concentrations of the analytical standards and commercial formulations of the herbicides atrazine (2 µg L− 1) and glyphosate (65 µg L− 1), in isolation and also in mixture (2 + 65 µg L− 1) on the microcrustacean Daphnia magna. Through chronic exposure (21 days) of two generations of organisms, effects on survival and reproductive capacity were observed, as well as responses regarding oxidative stress, determined through the analysis of biochemical biomarkers such as catalase and glutathione S-transferase. In the evaluation of the first generation of test organisms, no significant results related to biochemical biomarkers were observed, only effects over sexual maturation of organisms. However, in the second generation of exposed organisms, changes were observed in all parameters evaluated, with the mixture of herbicide active principles being the treatment responsible for more significant responses (p < 0.05). A statistical difference (p < 0.05) was also observed between analytical standards and commercial formulations, indicating that other components present in the formulations can change the toxicity of the products. Given the difficulty of estimating the effects of mixtures and considering that various stressors are found in the environment, our results support the need to carry out studies that address long-term effects and, above all, that verify what the impacts are across generations, so that the toxicity of products is not underestimated.

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