scholarly journals Effects of solvent—solvent fractionation on the total terpenoid content and in vitro anti‐inflammatory activity of Serevenia buxifolia bark extract

2021 ◽  
Author(s):  
Dieu‐Hien Truong ◽  
Nhat Thuy Anh Ta ◽  
Thanh Vy Pham ◽  
Tan Dat Huynh ◽  
Quoc Truong Giang Do ◽  
...  
Keyword(s):  
Inflammatory Activity ◽  
Bark Extract ◽  
Solvent Fractionation ◽  
Anti Inflammatory ◽  
Solvent Solvent

scholarly journals ANTI-INFLAMMATORY ACTIVITY OF KNEMA ATTENUATA (HOOK. F. AND THOMSON) WARB ETHANOLIC STEM BARK EXTRACT IN ALBINO WISTAR RATS

2019 ◽  
pp. 330-334
Author(s):  
SUPRIYA RAJA H
Keyword(s):  
Stem Bark ◽  
Inflammatory Activity ◽  
Assay Method ◽  
Bark Extract ◽  
Raw 264.7 ◽  
Myeloperoxidase Activity ◽  
Stem Bark Extract ◽  
Anti Inflammatory

Objective: Knema attenuata (Myristicaceae), popularly known as “wild nutmeg,” is an endemic tree species from Western Ghats, which has been used in folk medicine. Conventionally, the stem bark of K. attenuata is used for treating inflammatory conditions without any scientific information available for the same. The present study was undertaken to evaluate the anti-inflammatory activity of the ethanolic stem bark extract (ESBE) of K. attenuata using in vivo and in vitro screening models. Methods: The ethanolic extract of stem bark was prepared by soxhlation, and its cytotoxicity in RAW 264.7 cell line was assessed using MTT assay method. In vivo anti-inflammatory effect of extract was estimated in rats using carrageenan-induced paw edema model and cotton pellet-induced granuloma model. The in vitro anti-inflammatory activity of the extract was evaluated by cyclooxygenase and lipoxygenase inhibition assay, estimation of myeloperoxidase activity, and determination of cellular nitrite levels in lipopolysaccharide-stimulated RAW 264.7 macrophage cells. Results: Toxic symptoms were not observed for the ESBE. The extract demonstrated significant anti-inflammatory activity in both in vivo and in vitro models. The anti-inflammatory action exhibited by the extract was a result of the inhibition of leukocyte migration and nitric oxide pathway and partially by inhibition of mediators such as prostaglandins and leukotrienes. Conclusion: Findings from the study provide the evidence for the popular use of stem bark extract of K. attenuata as a potential anti-inflammatory agent.

scholarly journals In vitro anti-inflammatory activity of mangrove plant Rhizophora mucronata Lam. (Malpighiales: Rhizophoraceae)

2018 ◽  
Vol 5 (10) ◽  
pp. 417-426 ◽  
Author(s):  
Samanjit Kaur ◽  
M. Syed Ali ◽  
V. Anuradha ◽  
V. Suganya ◽  
A. Ashashalini ◽  
...  
Keyword(s):  
Protein Denaturation ◽  
Inflammatory Activity ◽  
Bark Extract ◽  
Root Extract ◽  
Rhizophora Mucronata ◽  
Membrane Stabilization ◽  
In Vivo Models ◽  
Maximum Inhibition ◽  
Anti Inflammatory

In the present study, analysis of in vitro inflammatory showed whole plant of Rhizophora mucronata Lam. (Malpighiales: Rhizophoraceae) can be the potent source. The data from this study showed that the R. mucronata leaf, bark and root extract could serve as an important anti-inflammatory agent. Moreover, among the three extracts, the stilt root and leaves extract showed highest anti inflammatory. In vitro anti-inflammatory activity of the selected plant extracts was evaluated using albumin denaturation, membrane stabilization and proteinase inhibitory assays. As part of the investigation on the mechanism of the anti-inflammation activity, ability of extract protein denaturation was studied. Maximum inhibition (296.26%) was observed from root extract followed by bark (259.48%) and leaf (237.62%). The extracts inhibited the heat induced hemolysis of RBCs to varying degree as show in table below. The maximum inhibition 284.17% was observed from bark extract followed by root (265.05%) and leaf (232.61%). It reveals that these phytochemical constituents are responsible to maximum protection of protein denaturation, albumin denaturation and membrane stabilization assay. The future work will be determination of anti-inflammatory and anti-arthritic activities by in vivo models.

scholarly journals EVALUATION OF ANTI-INFLAMMATORY AND ANTIDIABETIC ACTIVITY OF SIMAROUBA GLAUCA BARK EXTRACT: AN IN VITRO STUDY

2020 ◽  
pp. 74-78
Author(s):  
ALEESHA R ◽  
BHARAT MISHRA
Keyword(s):  
In Vitro Study ◽  
Inflammatory Activity ◽  
Antidiabetic Activity ◽  
Bark Extract ◽  
Potential Candidate ◽  
Bioactive Constituents ◽  
Ic50 Value ◽  
Anti Inflammatory ◽  
Simarouba Glauca

Objectives: The present study was envisaged to identify the effect of anti-inflammatory and antidiabetic activity for methanolic bark extracts of Simarouba glauca. Methods: The present study design was to evaluate the in vitro anti-inflammatory and in vitro antidiabetic activity of S. glauca methanolic bark extract. To examine the antidiabetic activity, the samples were studied for their effect on inhibition of alpha-amylase and glucose transport across the dialysis membrane. In vitro anti-inflammatory activity was evaluated using albumin denaturation assay and membrane stabilization method. Results: Our current results indicate that the various bioactive constituents detected in S. glauca may be responsible for its in vitro antidiabetic and anti-inflammatory effects. The ability of plant extract on anti-inflammatory activity showed that it was effective in inhibiting heat-induced albumin denaturation with an IC50 value of test and standard was found to be 46.42 μg/ml and 24.09 μg/ml. In addition to this, heat-induced hemolysis was also performed. The IC50 values of the test and standard were found to be 43.51 μg/ml and 21.41 μg/ml, respectively. The percentage inhibition of the test sample varied from the concentration range of 75 to 100 μg/ml. The IC50 value of the test and standard was found to be 19.08 μg/ml and 9.08 μg/ml, respectively. Conclusion: The findings of the present study concluded that S. glauca bark has the potential to treat diabetes and a novel natural anti-inflammatory agent as a good source. Thus, S. glauca may be a potential candidate for the development of future antidiabetic and anti-inflammatory compounds. However, still further studies and standardization of the plant research may be required to develop them as medicine.

scholarly journals In vitro and In vivo Anti-inflammatory Activities of Mesua ferrea Linn

2018 ◽  
Vol 10 (03) ◽  
Author(s):  
Krishna Chaithanya K ◽  
Gopalakrishnan V K ◽  
ZenebeHagos . ◽  
Nagaraju B ◽  
Kamalakararao K ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Wistar Rats ◽  
Inflammatory Activity ◽  
Raw 264.7 Cells ◽  
Bark Extract ◽  
Raw 264.7 ◽  
Dependent Manner ◽  
Anti Inflammatory

Objective: Mesuaferrea L is a medicinal plant belongs to the family Clusiace, it is extensively used in folk medicine for treatment of chronic inflammatory diseases.The present study was aimed to evaluate in vitro and in vivo anti-inflammatory activity of M. ferrea L. Methods: The in vitro anti-inflammatory activities such as nitric oxide, PGE2, pro-inflammatory cytokines (TNF-α and IL-1β) were studied in RAW 264.7 cells and in vivo studies were carried out on carrageenan -induced inflammation in Wistar rats. The sequentially extracted M. ferreaL bark extracts (MFBHE, MFBEE, and MFBME) exhibited inhibitory effects on pro-inflammatory mediators such as nitric oxide, prostaglandin E2, tumour necrosis factorαandinterleukin-1βproduction in concentration dependent manner in LPS induced RAW 264.7 cells andCarrageenan induced paw oedema in Wistar rats. Conclusion: The result of the present study indicated that M. ferrea L ethyl acetate bark extract exhibited significant in vitroand in vivoanti-inflammatory activity.

scholarly journals Phytochemical and Anti-Inflammatory Evaluation of Herbal Gel Prepared from Bark Extract of Mesua Ferrea Linn

2019 ◽  
Vol 9 (5-s) ◽  
pp. 53-56
Author(s):  
Harjeet Singh ◽  
B. K. Sarkar ◽  
Jagdish Chandra Arya ◽  
Shashi Pal ◽  
Ravi Kumar ◽  
...  
Keyword(s):  
Research Work ◽  
Stem Bark ◽  
Inflammatory Activity ◽  
Bark Extract ◽  
Phytochemical Analysis ◽  
Drug Study ◽  
Standard Drug ◽  
Ethanolic Extracts ◽  
Anti Inflammatory

The present research work involves formulation of herbal gel containing stem bark extracts of Mesua ferrea Linn and its evaluation for in vitro anti-inflammatory activity. The gel formulations were prepared using ethanolic extracts along with different polymer. The physiochemical parameters of formulations like; pH, viscosity and spreadability etc. also evaluated. Phytochemical analysis revealed presence of phenols, flavonoids and alkaloids, etc. The ethanolic extracts showed appreciable anti-inflammatory activity compared to the standard drug. Study confirmed that potential anti-inflammatory formulation can be developed from bark extract of Mesua ferrea Linn.   Keywords: Mesua ferrea Linn., Anti-inflammatory, Herbal Gel, Phytochemical, Albumin denaturation.

Potential anti-inflammatory activity of selected plants from Asteraceae family in in vitro models

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
B Michalak ◽  
ME Czerwińska ◽  
AK Kiss
Keyword(s):  
In Vitro Models ◽  
Inflammatory Activity ◽  
Anti Inflammatory ◽  
Asteraceae Family

In vitro anti-inflammatory activity of extracts from seeds of some Nigella species

Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
P Landa ◽  
P Marsik ◽  
T Vanek ◽  
L Kokoska
Keyword(s):  
Inflammatory Activity ◽  
Anti Inflammatory
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