scholarly journals Zonal regulation of gene expression during liver regeneration of urokinase transgenic mice

Hepatology ◽  
1999 ◽  
Vol 29 (4) ◽  
pp. 1106-1113 ◽  
Author(s):  
Stephanie Locaputo ◽  
Terri L. Carrick ◽  
Jorge A. Bezerra
Keyword(s):  
Gene Expression ◽  
Transgenic Mice ◽  
Liver Regeneration ◽  
Regulation Of Gene Expression

Investigation of cell physiology in the animal using transgenic technology

1992 ◽  
Vol 262 (2) ◽  
pp. C261-C275 ◽  
Author(s):  
A. P. Koretsky
Keyword(s):  
Gene Expression ◽  
Transgenic Mice ◽  
Regulation Of Gene Expression ◽  
Cell Physiology ◽  
Transgenic Technology ◽  
Cell Type ◽  
Mutant Proteins ◽  
The Past ◽  
Targeted Expression ◽  
Made In

Over the past 10 years significant progress has been made in techniques for manipulating the genome of the animal. Production of transgenic mice has led to important insights into the regulation of gene expression, the molecular basis of cancer, immunology, and developmental biology. The tools necessary to generate transgenic mice are becoming widely available, making it possible to study a variety of problems. In this review a description of the strategies being used to address problems of interest in cell physiology using transgenic mice is given. Elucidation of the rules governing the regulation of gene expression now permits the targeted expression of a protein to a particular organ or cell type within an organ. Overexpression of proteins, expression of foreign or mutant proteins, mislocalization of proteins, and directed elimination of proteins are all procedures that can now be used to generate interesting animal models for physiological studies. The applications of these techniques to a variety of problems in normal and abnormal physiology are discussed in this review.

A generic intron increases gene expression in transgenic mice

1991 ◽  
Vol 11 (6) ◽  
pp. 3070-3074
Author(s):  
T Choi ◽  
M Huang ◽  
C Gorman ◽  
R Jaenisch
Keyword(s):  
Gene Expression ◽  
Transgenic Mice ◽  
Transgene Expression ◽  
Integration Site ◽  
Regulation Of Gene Expression ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Transcription Unit ◽  
Histone H4 ◽  
Bacterial Gene

To investigate the role of splicing in the regulation of gene expression, we have generated transgenic mice carrying the human histone H4 promoter linked to the bacterial gene for chloramphenicol acetyltransferase (CAT), with or without a heterologous intron in the transcription unit. We found that CAT activity is 5- to 300-fold higher when the transgene incorporates a hybrid intron than with an analogous transgene precisely deleted for the intervening sequences. This hybrid intron, consisting of an adenovirus splice donor and an immunoglobulin G splice acceptor, stimulated expression in a broad range of tissues in the animal. Although the presence of the hybrid intron increased the frequency of transgenics with significant CAT activity, it did not affect the integration site-dependent variation commonly seen in transgene expression. To determine whether the enhancement is a general outcome of splicing or is dependent on the particular intron, we also produced equivalent transgenics carrying the widely used simian virus 40 small-t intron. We found that the hybrid intron is significantly more effective in elevating transgene expression. Our results suggest that inclusion of the generic intron in cDNA constructs may be valuable in achieving high levels of expression in transgenic mice.

scholarly journals CRE/CREB-Driven Up-Regulation of Gene Expression by Chronic Social Stress in CRE-Luciferase Transgenic Mice: Reversal by Antidepressant Treatment

PLoS ONE ◽  
2007 ◽  
Vol 2 (5) ◽  
pp. e431 ◽  
Author(s):  
Ulrike Böer ◽  
Tahseen Alejel ◽  
Stephan Beimesche ◽  
Irmgard Cierny ◽  
Doris Krause ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transgenic Mice ◽  
Social Stress ◽  
Antidepressant Treatment ◽  
Regulation Of Gene Expression ◽  
Chronic Social Stress

scholarly journals Differential regulation of the rat phosphoenolpyruvate carboxykinase gene expression in several tissues of transgenic mice.

1992 ◽  
Vol 12 (3) ◽  
pp. 1396-1403 ◽  
Author(s):  
C L Eisenberger ◽  
H Nechushtan ◽  
H Cohen ◽  
M Shani ◽  
L Reshef
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
Transgenic Mice ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Developmental Regulation ◽  
Regulation Of Gene Expression ◽  
Regulatory Sequences ◽  
Specific Expression ◽  
Endogenous Gene ◽  
Flanking Region

The selective expression of a unique copy gene in several mammalian tissues has been approached by studying the regulatory sequences needed to control expression of the rat phosphoenolpyruvate carboxykinase (PEPCK) gene in transgenic mice. A transgene containing the entire PEPCK gene, including 2.2 kb of the 5'-flanking region and 0.5 kb of the 3'-flanking region, exhibits tissue-specific expression in the liver, kidney, and adipose tissue, as well as the hormonal and developmental regulation inherent to endogenous gene expression. Deletions of the 5'-flanking region of the gene have shown the need for sequences downstream of position -540 of the PEPCK gene for expression in the liver and sequences downstream of position -362 for expression in the kidney. Additional sequences upstream of position -540 (up to -2200) are required for expression in adipose tissue. In addition, the region containing the glucocorticoid-responsive elements of the gene used by the kidney was identified. This same sequence was found to be needed specifically for developmental regulation of gene expression in the kidney and, together with upstream sequences, in the intestine. The apparently distinct sequence requirements in the various tissues indicate that the tissues use different mechanisms for expression of the same gene.

Hepatocyte growth factor in transgenic mice: Effects on hepatocyte growth, liver regeneration and gene expression

Hepatology ◽  
1994 ◽  
Vol 19 (4) ◽  
pp. 962-972 ◽  
Author(s):  
Goshi Shiota ◽  
Timothy C. Wang ◽  
Toshikazu Nakamura ◽  
Emmett V. Schmidt
Keyword(s):  
Gene Expression ◽  
Growth Factor ◽  
Transgenic Mice ◽  
Hepatocyte Growth Factor ◽  
Liver Regeneration ◽  
Hepatocyte Growth

MRI detection of transcriptional regulation of gene expression in transgenic mice

2007 ◽  
Vol 13 (4) ◽  
pp. 498-503 ◽  
Author(s):  
Batya Cohen ◽  
Keren Ziv ◽  
Vicki Plaks ◽  
Tomer Israely ◽  
Vyacheslav Kalchenko ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcriptional Regulation ◽  
Transgenic Mice ◽  
Regulation Of Gene Expression

Differential regulation of the rat phosphoenolpyruvate carboxykinase gene expression in several tissues of transgenic mice

1992 ◽  
Vol 12 (3) ◽  
pp. 1396-1403
Author(s):  
C L Eisenberger ◽  
H Nechushtan ◽  
H Cohen ◽  
M Shani ◽  
L Reshef
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
Transgenic Mice ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Developmental Regulation ◽  
Regulation Of Gene Expression ◽  
Regulatory Sequences ◽  
Specific Expression ◽  
Endogenous Gene ◽  
Flanking Region

The selective expression of a unique copy gene in several mammalian tissues has been approached by studying the regulatory sequences needed to control expression of the rat phosphoenolpyruvate carboxykinase (PEPCK) gene in transgenic mice. A transgene containing the entire PEPCK gene, including 2.2 kb of the 5'-flanking region and 0.5 kb of the 3'-flanking region, exhibits tissue-specific expression in the liver, kidney, and adipose tissue, as well as the hormonal and developmental regulation inherent to endogenous gene expression. Deletions of the 5'-flanking region of the gene have shown the need for sequences downstream of position -540 of the PEPCK gene for expression in the liver and sequences downstream of position -362 for expression in the kidney. Additional sequences upstream of position -540 (up to -2200) are required for expression in adipose tissue. In addition, the region containing the glucocorticoid-responsive elements of the gene used by the kidney was identified. This same sequence was found to be needed specifically for developmental regulation of gene expression in the kidney and, together with upstream sequences, in the intestine. The apparently distinct sequence requirements in the various tissues indicate that the tissues use different mechanisms for expression of the same gene.

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