scholarly journals Resolution of cellular compartments involved in membrane potential changes accompanying IgE-mediated degranulation of rat basophilic leukemia cells.

1984 ◽  
Vol 3 (3) ◽  
pp. 497-500 ◽  
Author(s):  
R. Sagi-Eisenberg ◽  
I. Pecht
Keyword(s):  
Membrane Potential ◽  
Leukemia Cells ◽  
Rat Basophilic Leukemia Cells ◽  
Ige Mediated ◽  
Cellular Compartments ◽  
Basophilic Leukemia

Effects of Various Cytochalasins on the IgE-Mediated Serotonin Release from Rat Basophilic Leukemia Cells

1985 ◽  
Vol 78 (3) ◽  
pp. 237-242 ◽  
Author(s):  
Reiko Teshima ◽  
Hideharu Ikebuchi ◽  
Setsuko Sekita ◽  
Shinsaku Natori ◽  
Tadao Terao
Keyword(s):  
Serotonin Release ◽  
Leukemia Cells ◽  
Rat Basophilic Leukemia Cells ◽  
Ige Mediated ◽  
Basophilic Leukemia

Phospholipase activation in the IgE-mediated and Ca2+ ionophore A23187-induced release of histamine from rat basophilic leukemia cells

1981 ◽  
Vol 212 (2) ◽  
pp. 572-580 ◽  
Author(s):  
Anne Mc Givney ◽  
Yutaka Morita ◽  
Fulton T. Crews ◽  
Fusao Hirata ◽  
Julius Axelrod ◽  
...  
Keyword(s):  
Leukemia Cells ◽  
Ionophore A23187 ◽  
Rat Basophilic Leukemia Cells ◽  
Ige Mediated ◽  
Basophilic Leukemia

scholarly journals IgE-mediated chemotaxis of rat basophilic leukemia cells towards specific antigen.

1983 ◽  
Vol 157 (6) ◽  
pp. 2166-2171 ◽  
Author(s):  
N Orida ◽  
J D Feldman ◽  
D H Katz ◽  
F T Liu
Keyword(s):  
Bovine Serum Albumin ◽  
Specific Antigen ◽  
Specific Ige ◽  
Leukemia Cells ◽  
Boyden Chamber ◽  
Antigen Specificity ◽  
Rat Basophilic Leukemia Cells ◽  
Ige Mediated ◽  
Basophilic Leukemia

We evaluated chemotactic properties of four sublines of rat basophilic leukemia cells using blindwell Boyden chamber assays. After sensitization with a mouse monoclonal IgE directed against dinitrophenyl (DNP), cells from sublines 2H3-C and 926a underwent chemotaxis toward DNP-bovine serum albumin (BSA) and sublines RBL-1 and 4A did not. Chemotactic responses required specific IgE and were determined by the IgE antigen specificity used for sensitization. The threshold for chemotaxis was on the order of 10(-10) M DNP-BSA. Release of incorporated [3H]-serotonin did not always parallel chemotactic responses, which suggests that chemotaxis and secretion may be two unlinked processes that occur during basophil activation. Our results predict a possible in vivo mechanism whereby specific chemotactic responses of basophils and other FcR epsilon-bearing cells are mediated via specific IgE bound to membrane FcR epsilon.

The distribution of antigen on the surface of RBL-2H3 rat basophilic leukemia cells observed by back-scattered electron imaging

1986 ◽  
Vol 44 ◽  
pp. 274-275
Author(s):  
R.F. Stump ◽  
J.R. Pfeiffer ◽  
JC. Seagrave ◽  
D. Huskisson ◽  
J.M. Oliver
Keyword(s):  
Cell Surface ◽  
Dynamic Properties ◽  
Leukemia Cells ◽  
Antigen Binding ◽  
Scattered Electron ◽  
Ige Receptor ◽  
Receptor Complexes ◽  
Rat Basophilic Leukemia Cells ◽  
Electron Imaging ◽  
Basophilic Leukemia

In RBL-2H3 rat basophilic leukemia cells, antigen binding to cell surface IgE-receptor complexes stimulates the release of inflammatory mediators and initiates a series of membrane and cytoskeletal events including a transformation of the cell surface from a microvillous to a lamellar topography. It is likely that dynamic properties of the IgE receptor contribute to the activation of these responses. Fewtrell and Metzger have established that limited crosslinking of IgE-receptor complexes is essential to trigger secretion. In addition, Baird and colleagues have reported that antigen binding causes a rapid immobilization of IgE-receptor complexes, and we have demonstrated an apparent increase with time in the affinity of IgE-receptor complexes for antigen.

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