CHEMICAL CONSTITUENTS OF NYLON-66 BEER ADSORBATE. II. THIN-LAYER CHROMATOGRAPHY OF THE ACIDIC AND ALKALINE HYDROLYSIS PRODUCTS

1971 ◽  
Vol 77 (1) ◽  
pp. 48-56 ◽  
Author(s):  
M. Dadić ◽  
J. E. A. Van Gheluwe ◽  
Z. Valyi
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Alkaline Hydrolysis ◽  
Chemical Constituents ◽  
Nylon 66 ◽  
Hydrolysis Products ◽  
Layer Chromatography

scholarly journals High Performance Thin Layer Chromatography Fingerprinting Analysis of Piper betle L. Leaves

2021 ◽  
pp. 8-15
Author(s):  
Ramdas N. Kale ◽  
Ravindra Y. Patil
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Methanolic Extract ◽  
Chemical Constituents ◽  
Piper Betle ◽  
Soxhlet Apparatus ◽  
Rf Values ◽  
Pharmacologically Active ◽  
Layer Chromatography

Introduction: Many modern medicines used today based on plants and plant products. Piper betle is generally known as the betle vine, it is an important medicinal and recreational plant. High performance thin layer chromatography (HPTLC) is an advanced powerful analytical method with more separation power, high performance and superior reproducibility than classic thin layer chromatography (TLC). A chromatographic fingerprint of a plant extract is a chromatographic pattern of some common chemical constituents of pharmacologically active and/or chemical characteristics. Chromatographic fingerprints are useful in authentication and identification of plant. Objectives:  Objectives of present research was to establish HPTLC fingerprinting of methanolic extract of Piper betle L. leaves. Materials and Methods: Methanolic extract of Piper betle leaves was prepared using soxhlet apparatus. HPTLC studies were performed using a CAMAG HPTLC system equipped with automatic TLC sampler-4 (ATS 4), TLC scanner 4, and vision CATS 3.0 software. Results: The study revealed the presence of alkaloids with Rf value 0.65, flavonoids with Rf values 0.19, 0.29, 0.72, 0.95., and phenolic compound with Rf value 0.7. Conclusion: The HPTLC fingerprinting profile developed for the methanolic extract of Piper betle L. leaves will help in proper identification of the plant.Piper betle

PHOSPHOLIPID COMPONENTS EXTRACTED FROM SASKATCHEWAN SOILS

1971 ◽  
Vol 51 (1) ◽  
pp. 19-22 ◽  
Author(s):  
C. G. KOWALENKO ◽  
R. B. McKERCHER
Keyword(s):  
Fatty Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Phosphatidyl Choline ◽  
Phosphatidyl Ethanolamine ◽  
Total Phospholipid ◽  
Plant Residues ◽  
Hydrolysis Products ◽  
Layer Chromatography ◽  
Choline Phosphatidyl

Soil phospholipids and their hydrolysis products, examined by thin-layer chromatography and selective sprays, revealed two major components, phosphatidyl ethanolamine and phosphatidyl choline. Phosphatidyl choline represented about 40% of the total phospholipid P and phosphatidyl ethanolamine about 30%. Small quantities of lyso-phosphatidyl choline were isolated from one soil. It is suggested that a study of the fatty acid constituents of phospholipids may indicate whether their source is largely from microorganisms or plant residues.

Determination of Linuron and Its Known and/or Suspected Metabolites in Crop Materials

1967 ◽  
Vol 50 (4) ◽  
pp. 911-917
Author(s):  
Stanley E Katz
Keyword(s):  
Thin Layer ◽  
Acid Hydrolysis ◽  
Thin Layer Chromatography ◽  
Aqueous Phase ◽  
Alkaline Hydrolysis ◽  
Ammonium Hydroxide ◽  
Enzymatic Digestion ◽  
Plant Materials ◽  
Layer Chromatography

Abstract Linuron and its known and/or suspected metabolites are extracted from crops with acetone. The acetone is evaporated, and the aqueous residue containing precipitated plant materials and linuron and metabolites is extracted several times with hexane to remove linuron and any 3,4-dichloroaniline, a metabolite. The 3,4-dichloroaniline is separated from the linuron by HC1 extraction. Other possible metabolites, 3-(3,4- dichlorophenyl)-l-methoxy urea, 3-(3,4-dichlorophenyl)- l-methyl urea, and 3-(3,4-dichlorophenyl) urea, remain in the aqueous phase which is made basic with ammonium hydroxide. These metabolites are extracted into hexane and identified by thin layer chromatography. Linuron and metabolites other than 3,4-dichloroaniline can be determined colorimetrically after acid hydrolysis to the aniline, followed by a diazotization reaction and coupling with iV-(l-naphthyl) ethylenediamine. Levels as low as 0.02 ppm linuron can be detected. Recoveries of 98.9% were found. Bound linuron can be determined by alkaline hydrolysis or by enzymatic digestion of crop material which frees the undegraded bound linuron

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