Thin Layer Chromatographic and Infra Red Spectral Evidence for the Presence of Phosphonolipids in Cicada oni

The phosphonolipids from whole Cicada oni have been isolated by preparative thin-layer chromatography (TLC) in methanol-water (2:1, v/v) and have been identified by TLC, infra-red spectroscopy, phosphono phosphorus determinations and elemental microanalyses. The presence has been confirmed of the phosphono analogues of phosphatidyl choline, phosphatidyl ethanolamine, sphingomyelin and of ceramide aminoethyl phosphonate.

ANALYSIS OF LIPIDS IN COTTON EXTRAFLORAL NECTAR1

Extrafloral cotton nectar subjected to gas chromatographic analysis revealed the presence of six fatty acids. Two saturated acids (palmitic and stearic) and four unsaturated acids (palmitoleic, oleic, linoleic, and linolenic), polar and neutral lipids, respectively, were identified. Thin layer chromotography separated six phospholipids including: phosphatidyl choline, phosphatidyl ethanolamine, phosphatidyl inositol, lysophosphatidyl choline, lysophosphatidyl ethanolamine, and an unknown. Fatty acid concentration was greatest in extrafloral nectar from young plants and decreased as the plants matured. The potential impact of extrafloral nectar lipid concentrations on predatory insects is discussed.

Tissue Thromboplastin: Relationship between Procoagulant Activity Lipid Composition and Morphology on Storage

Liquid suspensions of tissue extract thromboplastin gradually deteriorate on storage. Successive batches of standardised extracts of human brain prepared in this laboratory over a ten-year period have been studied by high pressure liquid chromatography. With storage, changes in lipid composition took place. Significant reductions in relative concentrations of phosphatidyl choline, phosphatidyl ethanolamine and phosphatidyl serine were found, while an increase in free fatty acids ana malonaldehyde was observed coincidental with decreasing procoagulant activity. Transmission electron microscopy showed changes in thromboplastin membrane structure which correlated with the observed biochemical characteristics and reducing procoagulant activity of extracts.

The phospholipids of Hansenula wingei, mating type 21

The phospholipid fraction of the yeast H. wingei was studied by a combination of DEAE-cellulose column chromatography and thin-layer chromatography. Phosphatidyl choline, phosphatidyl serine, and phosphatidyl ethanolamine were identified. The absence of phosphoinositide was noted. An unknown acidic phospholipid accounts for less than 1% of the total phosphorus. A cerebroside or cerebroside-like compound was provisionally identified.

PHOSPHOLIPID COMPONENTS EXTRACTED FROM SASKATCHEWAN SOILS

Soil phospholipids and their hydrolysis products, examined by thin-layer chromatography and selective sprays, revealed two major components, phosphatidyl ethanolamine and phosphatidyl choline. Phosphatidyl choline represented about 40% of the total phospholipid P and phosphatidyl ethanolamine about 30%. Small quantities of lyso-phosphatidyl choline were isolated from one soil. It is suggested that a study of the fatty acid constituents of phospholipids may indicate whether their source is largely from microorganisms or plant residues.

THE LIVER PHOSPHOLIPIDS OF THE DEVELOPING CHICK EMBRYO

The concentrations and fatty acid compositions of the individual phospholipids in the livers of chick embryos on the 13th, 15th, 17th, 19th, and 21st days of incubation were compared with the concentrations and fatty acid compositions of the individual yolk phospholipids. The liver phospholipids contained higher proportions of phosphatidyl ethanolamine, phosphatidyl serine, and diphosphatidyl glycerol, and lower proportions of phosphatidyl choline, than did the yolk phospholipids. There was a constant increment (0.96 mg/day) of phosphatidyl choline in the liver during the period of incubation studied. The phosphatidyl choline, phosphatidyl ethanolamine, and phosphatidyl serine of the liver generally contained higher concentrations of stearic, arachidonic, and docosahexaenoic acids, and lower concentrations of palmitic and oleic acids than did these phospholipid fractions in the yolk. The fatty acid compositions of the sphingomyelin in the liver and yolk were similar. The most pronounced changes in the fatty acid composition of the liver phospholipids during embryonic development were observed in the phosphatidyl choline fraction. These changes suggested that the α-palmitoyl-β-arachidonyl phosphatidyl choline in the liver was gradually replaced by α-stearoyl-β-linoleoyl phosphatidyl choline and a-stearoyl-β-docosahexaenoyl phosphatidyl choline.