Label ‐ free detection of living cervical cells based on microfluidic device with Terahertz spectroscopy

2021 ◽  
Author(s):  
Xiaoyue Yang ◽  
Mei Li ◽  
Qi Peng ◽  
Jian Huang ◽  
Lifen Liu ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Terahertz Spectroscopy ◽  
Label Free ◽  
Label Free Detection ◽  
Cervical Cells

scholarly journals Label-free detection of anti-estrogen receptor alpha and its binding with estrogen receptor peptide alpha by terahertz spectroscopy

RSC Advances ◽  
2017 ◽  
Vol 7 (39) ◽  
pp. 24338-24344 ◽  
Author(s):  
Mingliang Li ◽  
Tianying Chang ◽  
Dongshan Wei ◽  
Mingjie Tang ◽  
Shihan Yan ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Estrogen Receptor Alpha ◽  
Terahertz Spectroscopy ◽  
Hydration Shell ◽  
Spectroscopic Techniques ◽  
Shell Formation ◽  
Label Free ◽  
Binding Reaction ◽  
Receptor Alpha ◽  
Label Free Detection

Terahertz (THz) spectroscopic techniques were employed to study the hydration shell formation around anti-estrogen receptor alpha (AER-α) and to detect the binding reaction between AER-α and estrogen receptor peptide alpha (ERP-α).

Label-free detection of pepsinogen 1 and 2 by polyethylene coating Lamb microfluidic device

2019 ◽  
Vol 129 ◽  
pp. 231-237 ◽  
Author(s):  
Wei Wei ◽  
Wei Zhang ◽  
Chuanyu Li ◽  
Hui Kong ◽  
Zhen Guo ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Label Free ◽  
Polyethylene Coating ◽  
Label Free Detection

A microfluidic device with integrated impedance detection for λ-DNA

2003 ◽  
Vol 773 ◽  
Author(s):  
Myung-Il Park ◽  
Jonging Hong ◽  
Dae Sung Yoon ◽  
Chong-Ook Park ◽  
Geunbae Im
Keyword(s):  
Microfluidic Device ◽  
Electrochemical Impedance ◽  
Direct Detection ◽  
Full Potential ◽  
Label Free ◽  
Buffer Solution ◽  
Detection Systems ◽  
Significant Difference ◽  
Detection Of Biomolecules ◽  
Impedance Magnitude

AbstractThe large optical detection systems that are typically utilized at present may not be able to reach their full potential as portable analysis tools. Accurate, early, and fast diagnosis for many diseases requires the direct detection of biomolecules such as DNA, proteins, and cells. In this research, a glass microchip with integrated microelectrodes has been fabricated, and the performance of electrochemical impedance detection was investigated for the biomolecules. We have used label-free λ-DNA as a sample biomolecule. By changing the distance between microelectrodes, the significant difference between DW and the TE buffer solution is obtained from the impedance-frequency measurements. In addition, the comparison for the impedance magnitude of DW, the TE buffer, and λ-DNA at the same distance was analyzed.

scholarly journals The Role of Surface Chemistry in the Efficacy of Protein and DNA Microarrays for Label-Free Detection: An Overview

Polymers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1026
Author(s):  
Elisa Chiodi ◽  
Allison M. Marn ◽  
Matthew T. Geib ◽  
M. Selim Ünlü
Keyword(s):  
Surface Chemistry ◽  
Surface Functionalization ◽  
Dna Microarrays ◽  
Label Free ◽  
Polymeric Coatings ◽  
Label Free Detection ◽  
Particle Imaging ◽  
Single Particle Imaging ◽  
The Impact

The importance of microarrays in diagnostics and medicine has drastically increased in the last few years. Nevertheless, the efficiency of a microarray-based assay intrinsically depends on the density and functionality of the biorecognition elements immobilized onto each sensor spot. Recently, researchers have put effort into developing new functionalization strategies and technologies which provide efficient immobilization and stability of any sort of molecule. Here, we present an overview of the most widely used methods of surface functionalization of microarray substrates, as well as the most recent advances in the field, and compare their performance in terms of optimal immobilization of the bioreceptor molecules. We focus on label-free microarrays and, in particular, we aim to describe the impact of surface chemistry on two types of microarray-based sensors: microarrays for single particle imaging and for label-free measurements of binding kinetics. Both protein and DNA microarrays are taken into consideration, and the effect of different polymeric coatings on the molecules’ functionalities is critically analyzed.

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