Label‐free identification of human glioma xenograft of mouse brain with quantitative ultraviolet photoacoustic histology imaging

2022 ◽  
Author(s):  
Wei Song ◽  
Ya‐chao Wang ◽  
Huang Chen ◽  
Xiangzhu Li ◽  
Lingxiao Zhou ◽  
...  
Keyword(s):  
Mouse Brain ◽  
Label Free ◽  
Human Glioma ◽  
Glioma Xenograft

scholarly journals 1700 nm optical coherence microscopy enables minimally invasive, label-free, in vivo optical biopsy deep in the mouse brain

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Jun Zhu ◽  
Hercules Rezende Freitas ◽  
Izumi Maezawa ◽  
Lee-way Jin ◽  
Vivek J. Srinivasan
Keyword(s):  
Minimally Invasive ◽  
Mouse Brain ◽  
Numerical Aperture ◽  
Optical Biopsy ◽  
Optical Coherence ◽  
Label Free ◽  
Optical Coherence Microscopy ◽  
Minimal Invasiveness ◽  
Cortical Regions

AbstractIn vivo, minimally invasive microscopy in deep cortical and sub-cortical regions of the mouse brain has been challenging. To address this challenge, we present an in vivo high numerical aperture optical coherence microscopy (OCM) approach that fully utilizes the water absorption window around 1700 nm, where ballistic attenuation in the brain is minimized. Key issues, including detector noise, excess light source noise, chromatic dispersion, and the resolution-speckle tradeoff, are analyzed and optimized. Imaging through a thinned-skull preparation that preserves intracranial space, we present volumetric imaging of cytoarchitecture and myeloarchitecture across the entire depth of the mouse neocortex, and some sub-cortical regions. In an Alzheimer’s disease model, we report that findings in superficial and deep cortical layers diverge, highlighting the importance of deep optical biopsy. Compared to other microscopic techniques, our 1700 nm OCM approach achieves a unique combination of intrinsic contrast, minimal invasiveness, and high resolution for deep brain imaging.

A combination of IFN-β and temozolomide in human glioma xenograft models: implication of p53-mediated MGMT downregulation

2007 ◽  
Vol 61 (4) ◽  
pp. 653-659 ◽  
Author(s):  
Atsushi Natsume ◽  
Toshihiko Wakabayashi ◽  
Dai Ishii ◽  
Hideharu Maruta ◽  
Masazumi Fujii ◽  
...  
Keyword(s):  
Human Glioma ◽  
Glioma Xenograft ◽  
Xenograft Models

scholarly journals Proteomic Analysis of Mucopolysaccharidosis IIIB Mouse Brain

Biomolecules ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 355 ◽  
Author(s):  
Valeria De Pasquale ◽  
Michele Costanzo ◽  
Rosa Siciliano ◽  
Maria Mazzeo ◽  
Valeria Pistorio ◽  
...  
Keyword(s):  
Mouse Brain ◽  
Clinical Manifestations ◽  
Label Free ◽  
Wild Type ◽  
Proteomic Approach ◽  
Cytoskeletal Regulation ◽  
Proteome Profile ◽  
Subsequent Storage ◽  
And Control ◽  
Differentially Abundant Proteins

Mucopolysaccharidosis IIIB (MPS IIIB) is an inherited metabolic disease due to deficiency of α-N-Acetylglucosaminidase (NAGLU) enzyme with subsequent storage of undegraded heparan sulfate (HS). The main clinical manifestations of the disease are profound intellectual disability and neurodegeneration. A label-free quantitative proteomic approach was applied to compare the proteome profile of brains from MPS IIIB and control mice to identify altered neuropathological pathways of MPS IIIB. Proteins were identified through a bottom up analysis and 130 were significantly under-represented and 74 over-represented in MPS IIIB mouse brains compared to wild type (WT). Multiple bioinformatic analyses allowed to identify three major clusters of the differentially abundant proteins: proteins involved in cytoskeletal regulation, synaptic vesicle trafficking, and energy metabolism. The proteome profile of NAGLU−/− mouse brain could pave the way for further studies aimed at identifying novel therapeutic targets for the MPS IIIB. Data are available via ProteomeXchange with the identifier PXD017363.

scholarly journals Early detection of human glioma sphere xenografts in mouse brain using diffusion MRI at 14.1 T

2016 ◽  
Vol 29 (11) ◽  
pp. 1577-1589 ◽  
Author(s):  
P. Porcari ◽  
M. E. Hegi ◽  
H. Lei ◽  
M-F. Hamou ◽  
I. Vassallo ◽  
...  
Keyword(s):  
Early Detection ◽  
Mouse Brain ◽  
Diffusion Mri ◽  
Human Glioma

scholarly journals Alzheimer mouse brain tissue measured by time resolved fluorescence spectroscopy using single- and multi-photon excitation of label free native molecules

2017 ◽  
Vol 11 (1) ◽  
pp. e201600318 ◽  
Author(s):  
Bidyut Das ◽  
Lingyan Shi ◽  
Yury Budansky ◽  
Adrian Rodriguez-Contreras ◽  
Robert Alfano
Keyword(s):  
Fluorescence Spectroscopy ◽  
Brain Tissue ◽  
Mouse Brain ◽  
Label Free ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Photon Excitation

scholarly journals Label-free, multi-scale imaging of ex-vivo mouse brain using spatial light interference microscopy

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Eunjung Min ◽  
Mikhail E. Kandel ◽  
CheMyong J Ko ◽  
Gabriel Popescu ◽  
Woonggyu Jung ◽  
...  
Keyword(s):  
Mouse Brain ◽  
Ex Vivo ◽  
Interference Microscopy ◽  
Label Free ◽  
Multi Scale ◽  
Light Interference

Comparative localization of glioma-reactive monoclonal antibodies in vivo in an athymic mouse human glioma xenograft model

1987 ◽  
Vol 15 (1) ◽  
pp. 37-56 ◽  
Author(s):  
C.J. Wikstrand ◽  
R.E. McLendon ◽  
S. Carrel ◽  
J.T. Kemshead ◽  
J.-P. Mach ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Xenograft Model ◽  
Athymic Mouse ◽  
Human Glioma ◽  
Glioma Xenograft
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