scholarly journals Notochordal conditioned media from tissue increases proteoglycan accumulation and promotes a healthy nucleus pulposus phenotype in human mesenchymal stem cells

2011 ◽  
Vol 13 (3) ◽  
pp. R81 ◽  
Author(s):  
Devina Purmessur ◽  
Rachel M Schek ◽  
Rosalyn D Abbott ◽  
Bryan A Ballif ◽  
Karolyn E Godburn ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Nucleus Pulposus ◽  
Human Mesenchymal Stem Cells ◽  
Conditioned Media

scholarly journals Three-dimensional hypoxic culture of human mesenchymal stem cells encapsulated in a photocurable, biodegradable polymer hydrogel: A potential injectable cellular product for nucleus pulposus regeneration

2014 ◽  
Vol 10 (8) ◽  
pp. 3463-3474 ◽  
Author(s):  
Deepak Kumar ◽  
Irini Gerges ◽  
Margherita Tamplenizza ◽  
Cristina Lenardi ◽  
Nicholas R. Forsyth ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Nucleus Pulposus ◽  
Biodegradable Polymer ◽  
Three Dimensional ◽  
Human Mesenchymal Stem Cells ◽  
Polymer Hydrogel

scholarly journals Ischemic cardiac tissue conditioned media induced differentiation of human mesenchymal stem cells into early stage cardiomyocytes

2012 ◽  
Vol 64 (5) ◽  
pp. 563-575 ◽  
Author(s):  
Balasundari Ramesh ◽  
Dillip Kumar Bishi ◽  
Suneel Rallapalli ◽  
Sarasabarathi Arumugam ◽  
Kotturathu Mammen Cherian ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cardiac Tissue ◽  
Early Stage ◽  
Human Mesenchymal Stem Cells ◽  
Conditioned Media ◽  
Induced Differentiation

scholarly journals BMP3 Alone and Together with TGF-β Promote the Differentiation of Human Mesenchymal Stem Cells into a Nucleus Pulposus-Like Phenotype

2015 ◽  
Vol 16 (9) ◽  
pp. 20344-20359 ◽  
Author(s):  
Xiaopeng Zhou ◽  
Yiqing Tao ◽  
Chengzhen Liang ◽  
Yujie Zhang ◽  
Hao Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Nucleus Pulposus ◽  
Human Mesenchymal Stem Cells

scholarly journals 489 MICROARRAY PATHWAY ANALYSIS OF INHIBITION OF CHONDROGENESIS OF HUMAN MESENCHYMAL STEM CELLS BY OSTEOARTHRITIC SYNOVIUM DERIVED CONDITIONED MEDIA

2009 ◽  
Vol 17 ◽  
pp. S262-S263
Author(s):  
G.T. Heldens ◽  
E.L. Vitters ◽  
E. Verwiel ◽  
C. Gilissen ◽  
S. van Reijmersdal ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Pathway Analysis ◽  
Human Mesenchymal Stem Cells ◽  
Conditioned Media

Lower crosslinking density enhances functional nucleus pulposus-like matrix elaboration by human mesenchymal stem cells in carboxymethylcellulose hydrogels

2015 ◽  
Vol 104 (1) ◽  
pp. 165-177 ◽  
Author(s):  
Huizi A. Lin ◽  
Michelle S. Gupta ◽  
Devika M. Varma ◽  
M. Lane Gilchrist ◽  
Steven B. Nicoll
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Nucleus Pulposus ◽  
Human Mesenchymal Stem Cells ◽  
Crosslinking Density

scholarly journals Role of hypoxia and growth and differentiation factor-5 on differentiation of human mesenchymal stem cells towards intervertebral nucleus pulposus-like cells

2011 ◽  
Vol 21 ◽  
pp. 533-547 ◽  
Author(s):  
JV Stoyanov ◽  
◽  
B Gantenbein-Ritter ◽  
A Bertolo ◽  
N Aebli ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Nucleus Pulposus ◽  
Human Mesenchymal Stem Cells ◽  
Differentiation Factor

scholarly journals Human Levels of MMP-1 in Degenerated Disks Can Be Mitigated by Signaling Peptides from Mesenchymal Stem Cells

2020 ◽  
Vol 209 (2–3) ◽  
pp. 144-154
Author(s):  
Daphne Hingert ◽  
Phonphan Nawilaijaroen ◽  
Karin Ekström ◽  
Adad Baranto ◽  
Helena Brisby
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Vital Role ◽  
Conditioned Media ◽  
Intervertebral Disks ◽  
Signaling Peptides ◽  
Ecm Degradation ◽  
Ivd Degeneration

Degradation of extracellular matrix (ECM) in intervertebral disks (IVDs) during IVD degeneration plays a vital role in low back pain (LBP). In healthy IVDs, synthesis and degradation of ECM are kept in balance by matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs. MMPs are enzymes responsible for ECM degradation, and their expression levels are known to increase in degenerated disks. However, the exact pathophysiological concentration of MMP-1 in the degenerated disks of patients with chronic LBP has not been reported previously. Factors secreted by human mesenchymal stem cells (hMSCs) have shown positive results in cell therapy of degenerated disks. The aim of this study was to investigate the pathophysiological MMP-1 concentration (in ng/mL) in degenerated disk tissue and to evaluate if conditioned media (CM) from hMSCs could mitigate the effects of MMP-1 at the detected levels in a 3D in vitro disk cell (DC) pellet model. Tissue levels of MMP-1 were quantified in disk tissue collected from 6 chronic LBP patients undergoing surgery. DC pellet cultures were performed to investigate the effects of MMP-1 alone and the effects of conditioned media (CM) in the presence of MMP-1. MMP-1 was introduced in the pellets on day 14 at concentrations of 5, 50, or 100 ng/mL. The pellets were harvested on day 28 and evaluated for cell viability, proliferation, and ECM production. The mean concentration of MMP-1 in disk tissue was 151 ng/mL. Results from pellet cultures demonstrated a higher number of viable cells, glycosaminoglycan production, and ECM accumulation in the CM group even in the presence of MMP-1 compared to the controls. However, the level decreased with increasing MMP-1 concentration. The results demonstrated that CM has the ability to mitigate matrix degradation property of MMP-1 up to 50 ng/mL suggesting that CM could potentially be used to treat early stages of disk degeneration.

Analysis of the Effects of Low-Dose Radiation on Human Mesenchymal Stem Cells

2021 ◽  
Vol 65 (6) ◽  
pp. 5-10
Author(s):  
D. Usupzhanova ◽  
T. Astrelina ◽  
I. Kobzeva ◽  
V. Nikitina ◽  
Yu. Suchkova ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Conditioned Medium ◽  
Adaptive Response ◽  
Proliferative Activity ◽  
Human Mesenchymal Stem Cells ◽  
Conditioned Media ◽  
X Ray ◽  
Cultivation In Vitro

Purpose: The aim of the study was to study the effect of low X-ray doses on human mesenchymal stem cells (MSCs) in long-term cultivation in vitro. Material and methods: MSCs of the mucosal gum tissue of human were used. Cells were irradiated using an RUST-M1 X-ray unit (Russia) at doses of 50, 80, 100, 250 and 1000 mGy (dose rate40 mGy/min) and then cultivated according to standard methods. Immunological characteristics and viability of MSCs were evaluated on a FACSCanto II flow cytometer (Becton Dickinson CA, USA) for early and late passengers. Proliferative activity (PA) was evaluated using an xCelligence real-time cell analyzer (ACEA Biosciencs, Inc.). Results: It was shown that the proliferative activity (PA) of MSCs of the mucosal gum tissue which were irradiated at dose 50 mGy is comparable with the control group in long-term cultivation while doses of 100 and 250 mGy showed a decrease of PA. Also non-irradiated MSCs showed a significant decrease of the PA during cultivation in a conditioned medium from cells that received dose of 1000 mGy and an increase of PA during cultivation in a conditioned medium of cells that received doses of 50, 100 and 250 mGy. The cells were previously irradiated at dose 250 mGy showed adaptive response during cultivation in conditioned medium from cells that received dose of 1000 mGy. Сonclusion: The assessment of the effects of low radiation doses was focused on the bystander effect in the presented study. It was noted after adding conditioned media from irradiated cells to previously irradiated and non-irradiated MSCs. The bystander effects for low and high doses are different and their biological meaning requires further study. The phenomenon of adaptive response was shown after addition conditioned media from cells irradiated at dose 1000 mGy to pre-irradiated MSCs received a dose of 250 mGy. The obtained result leads to the conclusion that the effects of low doses can be positive. Thus, the results of study mainly correspond to the threshold nonlinear concept, according to which the effect is not proportional to the received radiation dose.

scholarly journals Influence of different commercial scaffolds on the in vitro differentiation of human mesenchymal stem cells to nucleus pulposus-like cells

2011 ◽  
Vol 21 (S6) ◽  
pp. 826-838 ◽  
Author(s):  
Alessandro Bertolo ◽  
Marco Mehr ◽  
Niklaus Aebli ◽  
Martin Baur ◽  
Stephen J. Ferguson ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Nucleus Pulposus ◽  
Human Mesenchymal Stem Cells ◽  
In Vitro Differentiation
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