Effects of platelet-rich plasma on biological activity and bone regeneration of brushite-based calcium phosphate cement

The management and treatment of dental and craniofacial injuries have continued to evolve throughout the last several decades. Limitations with autograft, allograft, and synthetics created the need for more advanced approaches in tissue engineering. Calcium phosphate cements (CPC) are frequently used to repair bone defects. Since their discovery in the 1980s, extensive research has been conducted to improve their properties, and emerging evidence supports their increased application in bone tissue engineering. This review focuses on the up-to-date performance of calcium phosphate cement (CPC) scaffolds and upcoming promising dental and craniofacial bone regeneration strategies. First, we summarized the barriers encountered in CPC scaffold development. Second, we compiled the most up to date in vitro and in vivo literature. Then, we conducted a systematic search of scientific articles in MEDLINE and EMBASE to screen the related studies. Lastly, we revealed the current developments to effectively design CPC scaffolds and track the enhanced viability and therapeutic efficacy to overcome the current limitations and upcoming perspectives. Finally, we presented a timely and opportune review article focusing on the significant potential of CPC scaffolds for dental and craniofacial bone regeneration, which will be discussed thoroughly. CPC offers multiple capabilities that may be considered toward the oral defects, expecting a future outlook in nanotechnology design and performance.

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Incorporation of BMP-2 loaded collagen conjugated BCP granules in calcium phosphate cement based injectable bone substitutes for improved bone regeneration

Materials Science and Engineering C ◽

10.1016/j.msec.2017.03.296 ◽

2017 ◽

Vol 77 ◽

pp. 713-724 ◽

Cited By ~ 22

Author(s):

Gun Hee Lee ◽

Preeti Makkar ◽

Kallyanshis Paul ◽

ByongTaek Lee

Keyword(s):

Calcium Phosphate ◽

Bone Regeneration ◽

Calcium Phosphate Cement ◽

Bone Substitutes

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Properties of osteoconductive biomaterials: Calcium phosphate cement with different ratios of platelet-rich plasma as identifiers

Materials Science and Engineering C ◽

10.1016/j.msec.2013.04.042 ◽

2013 ◽

Vol 33 (6) ◽

pp. 3537-3544 ◽

Cited By ~ 27

Author(s):

Chia-Ling Ko ◽

Wen-Cheng Chen ◽

Jian-Chih Chen ◽

Ying- Hui Wang ◽

Chi-Jen Shih ◽

...

Keyword(s):

Calcium Phosphate ◽

Calcium Phosphate Cement ◽

Platelet Rich Plasma

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Bone Regeneration Using Adipose-Derived Stem Cells in Injectable Thermo-Gelling Hydrogel Scaffold Containing Platelet-Rich Plasma and Biphasic Calcium Phosphate

International Journal of Molecular Sciences ◽

10.3390/ijms19092537 ◽

2018 ◽

Vol 19 (9) ◽

pp. 2537 ◽

Cited By ~ 15

Author(s):

Han Liao ◽

Ming-Jin Tsai ◽

Manuri Brahmayya ◽

Jyh-Ping Chen

Keyword(s):

Stem Cells ◽

Cell Proliferation ◽

Calcium Phosphate ◽

Bone Regeneration ◽

Biphasic Calcium Phosphate ◽

Platelet Rich Plasma ◽

Bone Matrix ◽

Calcium Deposition ◽

Adipose Derived Stem Cells ◽

Hydrogel Scaffold

For bone regeneration, a biocompatible thermo-gelling hydrogel, hyaluronic acid-g-chitosan-g-poly(N-isopropylacrylamide) (HA-CPN) was used as a three-dimensional organic gel matrix for entrapping rabbit adipose-derived stem cells (rASCs). Biphasic calcium phosphate (BCP) ceramic microparticles were embedded within the gel matrix as a mineralized bone matrix, which was further fortified with platelet-rich plasma (PRP) with osteo-inductive properties. In vitro culture of rASCs in HA-CPN and HA-CPN/PRP/BCP was compared for cell proliferation and osteogenic differentiation. Overall, HA-CPN/PRP/BCP was a better injectable cell carrier for osteogenesis of rASCs with increased cell proliferation rate and alkaline phosphatase activity, enhanced calcium deposition and mineralization of extracellular matrix, and up-regulated expression of genetic markers of osteogenesis. By implanting HA-CPN/PRP/BCP/rASCs constructs in rabbit critical size calvarial bone defects, new bone formation at the defect site was successfully demonstrated from computed tomography, and histological and immunohistochemical analysis. Taken together, by combining PRP and BCP as the osteo-inductive and osteo-conductive factor with HA-CPN, we successfully demonstrated the thermo-gelling composite hydrogel scaffold could promote the osteogenesis of rASCs for bone tissue engineering applications.

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