Exosome mediated transfer of miRNA‐140 promotes enhanced chondrogenic differentiation of bone marrow stem cells for enhanced cartilage repair and regeneration

2020 ◽  
Vol 121 (7) ◽  
pp. 3642-3652 ◽  
Author(s):  
Gi Won Lee ◽  
Muthukumar Thangavelu ◽  
Min Joung Choi ◽  
Eun Yeong Shin ◽  
Han Sol Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Cartilage Repair ◽  
Chondrogenic Differentiation ◽  
Bone Marrow Stem Cells

scholarly journals Hydrostatic pressure promotes chondrogenic differentiation and microvesicle release from human embryonic and bone marrow stem cells

2021 ◽  
pp. 2100401
Author(s):  
Lu Luo ◽  
Nicola C. Foster ◽  
Kenny L. Man ◽  
Mathieu Brunet ◽  
David A. Hoey ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Hydrostatic Pressure ◽  
Chondrogenic Differentiation ◽  
Bone Marrow Stem Cells

scholarly journals Chondrogenic Differentiation of Human Bone Marrow Stem Cells in Transwell Cultures: Generation of Scaffold-Free Cartilage

Stem Cells ◽  
2007 ◽  
Vol 25 (11) ◽  
pp. 2786-2796 ◽  
Author(s):  
Alan D. Murdoch ◽  
Lisa M. Grady ◽  
Matthew P. Ablett ◽  
Theoni Katopodi ◽  
Roger S. Meadows ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Chondrogenic Differentiation ◽  
Bone Marrow Stem Cells ◽  
Human Bone ◽  
Human Bone Marrow

scholarly journals Effect of a combination of TGFβ and IGF growth factors on the chondrogenic potential of mesenchymal bone marrow stem cells

2021 ◽  
Vol 2 (216) ◽  
pp. 78-83
Author(s):  
Anna Zhernosechenko ◽  
◽  
Yanina Isaykina ◽  
Tatiana Filipovich
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Bone Marrow ◽  
Growth Factors ◽  
Chondrogenic Differentiation ◽  
Bone Marrow Stem Cells ◽  
Suppression Effect ◽  
Chondrogenic Potential ◽  
2D And 3D

The authors studied the influence of the combination of TGF? and IGF growth factors, as well as the differentiation time, on the induction of MSC chondrogenesis in vitro. It is proved that MSCs located in 2D and 3D systems, when exposed to TGF?/ IGF, showed the signs of early chondroblast-like cells in 7 days. The TGF?/ IGF used for the induction of MSCs is more preferred, because it results in a more pronounced hypertrophic-suppression effect. The absence of significant differences in gene expression (excepting Sox9) on the 7th and 21st days of chondrogenic differentiation allows the process to be reduced in vitro to 7 days.

Controlled Differentiation of Mesenchymal Stem Cells into Hyaline Cartilage in miR-140-Activated Collagen Hydrogel

Cartilage ◽  
2021 ◽  
pp. 194760352110476
Author(s):  
Karthikeyan Rajagopal ◽  
Porkizhi Arjunan ◽  
Srujan Marepally ◽  
Vrisha Madhuri
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cartilage Repair ◽  
Chondrogenic Differentiation ◽  
Hyaline Cartilage ◽  
Cartilage Tissue ◽  
Bone Marrow Mscs ◽  
Rt Pcr ◽  
Collagen Hydrogel

Objective Hypertrophic cartilage formation is a major setback in mesenchymal stem cells (MSCs)–mediated cartilage repair, and overcoming it requires optimization of differentiation. Here, we tested the miR-140 activated collagen hydrogel for the chondrogenic differentiation of MSCs and to produce hyaline cartilage. Methods Bone marrow MSCs isolated from 3 patients were pretreated with miR-140 and then chondrogenic differentiated. The 3-dimensional (3D) transfection potential of 5 different transfection reagents (Polyethylenimine, Lipofectamine, TransIT-X2, Amide:Cholesterol-based liposomes [AmC] and AmC pegylated with Tocofersolan [AmCTOC]) was compared and the reagent that showed higher green fluorescent protein (GFP) expression was selected. Finally, the collagen hydrogel was activated using miR-140-transfection complex and sustained delivered to MSCs during chondrogenic differentiation. After differentiation, the outcome was assessed by reverse transcription–polymerase chain reaction (RT-PCR), histology, immunohistochemistry, and compared with scrambled miRNA treated control. Results Pretreatment of MSCs with miR-140 significantly increased the expression of cartilage-specific genes ( COL2A1, SOX9, and ACAN) with reduced hypertrophic chondrocyte ( COL10A1) marker expression and better safranin-O staining than the control. The AmCTOC liposome showed a significant increase in 3D transfection of GFP expressing plasmid than the others. Furthermore, the knockdown of GAPDH using siRNA in HEK cells and expression of GFP mRNA in human bone marrow MSCs confirmed the 3D-transfection efficiency of AmCTOC. The sustained delivery of miR-140 using activated matrix formed a hyaline cartilage-like tissue with minimal COL10A1 expression in RT-PCR and immunohistochemistry. Conclusion Our results demonstrated the therapeutic potential of miR-140-activated hydrogel for MSCs-based cartilage tissue engineering, which could also be used for endogenous stem cells–mediated cartilage repair.

Bone marrow stem cells make hepatocytes with replicative potential

2001 ◽  
Vol 120 (5) ◽  
pp. A62-A62
Author(s):  
S FORBES ◽  
M ALISON ◽  
K HODIVALADILKE ◽  
R JEFFERY ◽  
R POULSOM ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Bone Marrow Stem Cells

Autologous 5-azacytidine-cultured bone-marrow stem cells in surgical treatment of heart failure

2008 ◽  
Vol 7 ◽  
pp. 114-115
Author(s):  
R AKCHURIN ◽  
T RAKHMATZADE ◽  
E SKRIDLEVSKAYA ◽  
L SAMOYLENKO ◽  
V SERGIENKO ◽  
...  
Keyword(s):  
Heart Failure ◽  
Stem Cells ◽  
Bone Marrow ◽  
Surgical Treatment ◽  
Bone Marrow Stem Cells
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