scholarly journals New enzymatic assay for serum urea nitrogen using urea amidolyase

2003 ◽  
Vol 17 (2) ◽  
pp. 52-56 ◽  
Author(s):  
Shigeki Kimura ◽  
Shigeru Iyama ◽  
Yoshihisa Yamaguchi ◽  
Yuzuru Kanakura
2011 ◽  
Vol 356-360 ◽  
pp. 142-145
Author(s):  
Jian Hui Wang ◽  
Dong Min Liu ◽  
Yong Le Liu ◽  
Chi Lin Li ◽  
Fa Xiang Wang ◽  
...  

The objective of this study was to determine the effects of chlorogenic acid (CA) and bamboo charcoal particles activated by microwave (BCP) on performance, serum urea nitrogen and husbandry environment of broilers. The diets were devoid of antibiotic growth promoters (AGP). Two hundred and forty Avian broilers were allocated to a 2 × 2 factorial experiment involving two levels of CA (0 or 1000 mg CA/kg feed), and two levels of BCP supplementation (0 or 100 mg/kg feed). The diets and water were offered ad libitum. The growth performance and serum urea nitrogen was significantly increased when CA supplemented (P<0.05), while BCP significantly affects the ammonia concentration of the husbandry environment (P<0.05). These results indicate that CA and BCP combination can effectively improve the performance and husbandry environment.


1993 ◽  
Vol 76 (12) ◽  
pp. 3742-3746 ◽  
Author(s):  
James D. Ferguson ◽  
David T. Galligan ◽  
Terry Blanchard ◽  
Matthew Reeves

1971 ◽  
Vol 17 (9) ◽  
pp. 891-895 ◽  
Author(s):  
Donald R Wybenga ◽  
John Di Giorgio ◽  
Vincent J Pileggi

Abstract A simple method is described for directly determining serum urea nitrogen, wtihout deproteinization. The urea in 20 µl of serum or plasma is reacted with diacetylmonoxime in the presence of thiosemicarbazide and cadmium ion under acid conditions. The absorbance of the resulting rose-purple solution is measured at 540 nm. Results agreed excellently with those obtained by a reference method that makes use of urease and the Berthelot phenate—hypochlorite reaction for ammonia. An automated adaptation utilizing the AutoAnalyzer is also described, which eliminates the need for a dialyzer. A further advantage is that the same reagents are used for both methods.


1966 ◽  
Vol 12 (3) ◽  
pp. 151-157 ◽  
Author(s):  
Alan A Wilcox ◽  
Wallace E Carroll ◽  
Rex E Sterling ◽  
H A Davis ◽  
Arnold G Ware

Abstract A method is presented for the automated determination of urea nitrogen using the Berthelot reaction for measuring the ammonia released by the enzymatic action of urease. The method compares favorably with the automated diacetyl monoxime method and employs less dangerous and less expensive reagents.


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