scholarly journals Evaluation of direct antifungal susceptibility testing methods of Candida spp. from positive blood culture bottles

2017 ◽  
Vol 32 (3) ◽  
pp. e22297 ◽  
Author(s):  
Yasemin Oz ◽  
Egemen Gokbolat
Keyword(s):  
Blood Culture ◽  
Positive Blood Culture ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
Testing Methods ◽  
Candida Spp

scholarly journals Antifungal Susceptibility Testing: Practical Aspects and Current Challenges

2001 ◽  
Vol 14 (4) ◽  
pp. 643-658 ◽  
Author(s):  
John H. Rex ◽  
Michael A. Pfaller ◽  
Thomas J. Walsh ◽  
Vishnu Chaturvedi ◽  
Ana Espinel-Ingroff ◽  
...  
Keyword(s):  
Amphotericin B ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
Testing Methods ◽  
Clinical Value ◽  
Candida Spp ◽  
Testing Procedures ◽  
Reference Methods ◽  
Interlaboratory Reproducibility

SUMMARY Development of standardized antifungal susceptibility testing methods has been the focus of intensive research for the last 15 years. Reference methods for yeasts (NCCLS M27-A) and molds (M38-P) are now available. The development of these methods provides researchers not only with standardized methods for testing but also with an understanding of the variables that affect interlaboratory reproducibility. With this knowledge, we have now moved into the phase of (i) demonstrating the clinical value (or lack thereof) of standardized methods, (ii) developing modifications to these reference methods that address specific problems, and (iii) developing reliable commercial test kits. Clinically relevant testing is now available for selected fungi and drugs: Candida spp. against fluconazole, itraconazole, flucytosine, and (perhaps) amphotericin B; Cryptococcus neoformans against (perhaps) fluconazole and amphotericin B; and Aspergillus spp. against (perhaps) itraconazole. Expanding the range of useful testing procedures is the current focus of research in this area.

scholarly journals Antifungal susceptibility testing.

1993 ◽  
Vol 6 (4) ◽  
pp. 367-381 ◽  
Author(s):  
J H Rex ◽  
M A Pfaller ◽  
M G Rinaldi ◽  
A Polak ◽  
J N Galgiani
Keyword(s):  
Susceptibility Testing ◽  
Incubation Temperature ◽  
Clinical Laboratory ◽  
Antifungal Susceptibility ◽  
Inoculum Size ◽  
Antifungal Susceptibility Testing ◽  
National Committee ◽  
Candida Spp ◽  
Interlaboratory Variability ◽  
Future Work

Unlike antibacterial susceptibility testing, reliable antifungal susceptibility testing is still largely in its infancy. Many methods have been described, but they produce widely discrepant results unless such factors as pH, inoculum size, medium formulation, incubation time, and incubation temperature are carefully controlled. Even when laboratories agree upon a common method, interlaboratory agreement may be poor. As a result of numerous collaborative projects carried out both independently and under the aegis of the Subcommittee on Antifungal Susceptibility Testing of the National Committee for Clinical Laboratory Standards, the effects of varying these factors have been extensively studied and a standard method which minimizes interlaboratory variability during the testing of Candida spp. and Cryptococcus neoformans has been proposed. This review summarizes this work, reviews the strengths and weaknesses of the proposed susceptibility testing standard, and identifies directions for future work.

scholarly journals Corrigendum: Growth kinetics in Candida spp.: Differences between species and potential impact on antifungal susceptibility testing as described by the EUCAST.

2019 ◽  
Vol 57 (5) ◽  
pp. e8-e10 ◽  
Author(s):  
María Ángeles Bordallo-Cardona ◽  
Carlos Sánchez-Carrillo ◽  
Patricia Muñoz ◽  
Emilio Bouza ◽  
Pilar Escribano ◽  
...  
Keyword(s):  
Growth Kinetics ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
Candida Spp ◽  
Potential Impact

scholarly journals Evaluation of the “Dip Effect” Phenomenon in Antifungal Susceptibility Testing of Candida spp. against Echinocandins by Use of Gradient Concentration Strips

2015 ◽  
Vol 53 (11) ◽  
pp. 3654-3659 ◽  
Author(s):  
Maria Siopi ◽  
Marilena Tsala ◽  
Nikolaos Siafakas ◽  
Loukia Zerva ◽  
Joseph Meletiadis
Keyword(s):  
Candida Albicans ◽  
Candida Tropicalis ◽  
Candida Glabrata ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
Practical Approach ◽  
Candida Spp ◽  
Content Type ◽  
Inhibition Zones

The “dip effect” phenomenon complicates antifungal susceptibility testing with gradient concentration strips. Of 60Candidaisolates tested with the three echinocandins, this phenomenon was observed only for caspofungin with most (>90%)Candida albicans,Candida glabrata, andCandida tropicalisisolates and for isolates with CLSI MICs of ≤0.25 mg/liter. In order to facilitate MIC determination, a practical approach was developed using the inhibition zones at 32, 8, 2, and 1 mg/liter, increasing the agreement with the CLSI method >86%.

scholarly journals Antifungal susceptibility testing of candida – Methods and issues in current practice

2019 ◽  
Vol 10 (3) ◽  
pp. 1778-1784
Author(s):  
Lakshmi Krishnasamy ◽  
Priya Santharam ◽  
Chitralekha Saikumar
Keyword(s):  
Current Practice ◽  
Antifungal Agents ◽  
Susceptibility Testing ◽  
Fungal Infections ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
Antifungal Drugs ◽  
Testing Methods ◽  
Antifungal Drug Resistance ◽  
Resistant Strains

Despite the availability of many antifungal drugs in clinical practice, the occurrence of antifungal drug resistance is on the rise. Since the antifungal susceptibility testing (AFST) is not done routinely in many of the microbiology laboratories, it is very difficult to determine which antifungal agent is very effective for a particular infection. There is a real need for precise, reproducible and extrapolative antifungal susceptibility testing methods to aid the therapeutic management. The practice of empirical treatment for fungal infections further promotes the emergence of resistant strains. The AFST practice would essentially help the clinicians in appropriate decision making. Although conventional AFST methods are somewhat cumbersome, many novel AFST methods are currently available in many laboratory settings which would provide a quicker result many times. In essence, the application of AFST along with identification of the fungus up to species level would definitely be very helpful in selecting the primary antifungal agents for treatment especially in difficult to manage and invasive fungal infections. This review will throw light on the various AFST methods available and their issues in the current practice.

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